甲醛/二氧六环预处理慈竹制单糖及乳酸

doi:10.3969/j.issn.0253-2417.2022.04.015

Abstract

Abstract:

Using bamboo as raw material, the effect of formaldehyde/1, 4-dioxane pretreatment on the yield of enzymatic hydrolysis sugar from bamboo cellulose was studied. The producing process of lactic acid through fermentation with the enzymatic hydrolysate was also investigated. The results showed that the pretreatment could remove lignin and greatly increase the cellulose content of the pretreated bamboo substrate, with cellulose content reaching 75.6%-90.7%. The highest yield of cellulose-to-glucose by enzymatic hydrolysis after pretreatment was 92.8%. The enzymatic hydrolysate could be directly used for fermentation to produce lactic acid, and the yield of the glucose to lactic acid could reach 73.2%. The changes of bamboo structure before and after pretreatment were observed by scanning electron microscope(SEM), X-ray diffraction(XRD) and ion chromatograph. After pretreatment, the bamboo surface curled, the wax layer on the surface of bamboo was almost completely dissolved and removed, and the structure became loose.

Key words: bamboo, formaldehyde/1, 4-dioxane, pretreatment, enzymatic hydrolysis, lactic acid

CLC Number:

TQ35

Tengfei MA, Yawei ZHAN, Yue LIU, Zhiqiang LI. Formaldehyde/Dioxane Pretreatment on Bamboo for Producing Monosaccharide and Lactic Acid[J]. Chemistry and Industry of Forest Products, 2022, 42(4): 101-106.

Figures/Tables 5

Table 1

Fig.1

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Table 2

Table 3

References 16

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序号 No.	甲醛/mL formaldehyde	得率/% yield	纤维素/% cellulose	木质素/% lignin	半纤维素/% hemicellulose	合计/% sum
1	未处理竹材 untreated bamboo		41.2	22.1	22.7	86.0
2	0	51.7±1.2	89.0±4.5	4.6±0.1	—	93.6
3	3.0	55.3±0.3	90.7±3.5	9.5±0.1	—	100.2
4	4.0	53.4±1.9	86.9±4.3	8.7±0.3	—	95.6
5	4.5	53.7±0.6	82.9±3.5	8.4±0.6	—	91.3
6	5.0	52.2±0.7	75.6±5.5	8.2±0.4	—	83.8

甲醛用量/mL formaldehyde	葡萄糖得率/% glucose yield	总糖得率/% total sugar yield
未处理竹材 untreated bamboo	1.67	10.25
0.0	33.2±1.0	94.0±2.7
3.0	7.2±1.8	31.9±7.8
4.0	5.0±0.6	22.8±5.7
4.5	5.1±0.6	22.3±3.0
5.0	3.5±0.1	27.1±3.7

样品 sample	HCl/mL	H₂SO₄/mL	甲醛/mL formaldehyde	酶水解葡萄糖得率/% glucose yield	葡萄糖发酵转化率/% glucose conversion	乳酸得率/% lactic acid yield
1	2.1	—	5.0	8.1	92.7	30.9
2	2.1	—	—	92.8	99.8	25.5
3	—	1.1	5.0	3.6	100	73.2
4	—	1.1	—	85.1	99.7	37.7

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Formaldehyde/Dioxane Pretreatment on Bamboo for Producing Monosaccharide and Lactic Acid

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