党参多糖的大孔树脂纯化及其美白、吸湿保湿性能

doi:10.3969/j.issn.0253-2417.2023.03.010

Abstract

Abstract:

On the basis of static experiments, the purification effects of polysaccharides on 14 kinds of macroporous adsorption resins(S-8, ADS-F8, LSA-21, LSA-10, HP-20, ADS-17, NKA-9, DM130, AB-8, X-5, H103, D101, XAD-8, DA201) from Codonopsis pilosula(Franch.) Nannf. extraction were compared, and the better resin ADS-F8 was selected for dynamic adsorption. Based on the single factor experiments, the weighted comprehensive score of polysaccharides retention rate, decolorization rate and protein removal rate were used as the response values, and the response surface methodology was used to optimize the purification conditions of C. pilosula polysaccharide by macroporous resin. Meanwhile, the whitening activity, moisture absorption and moisture retention of the purified polysaccharides were tested. The results showed that the optimal purification conditions were as follows: loading concentration of 1 g/L, eluting volume of 150 mL, flow rate of 100 mL/h, and sample volume of 150 mL. Under these conditions, the comprehensive score of purification was (73.12±0.06)%(n=3), which was close to the predicted value of 73.02%. The purified polysaccharides had favorable whitening activity, and the half inhibitory mass concentration(IC₅₀) value of tyrosinase monophenase was 3.20 g/L, which was similar to the inhibitory effect of whitening agent phenylethylresorcinol. The IC₅₀ value of bisphenolase was 7.96 g/L, which is higher than phenylethylresorcinol of 0.73 g/L. When the air humidity was 43%, the purified polysaccharide showed strong hygroscopicity, and the highest moisture absorption rate was 123.01% at 12 h. In four air humidity conditions, the purified polysaccharides, crude polysaccharides and their mixture with a volume ratio 1∶1 to glycerol all had high moisture retention, especially, the moisturizing property of the mixture was better, and the moisturizing rate reached 99.95% within 12 h, which was significantly better than that of purified polysaccharide and crude polysaccharide.

Key words: Codonopsis pilosula(Franch.) Nannf., polysaccharides, macroporous adsorption resin, response surface method, whitening activity, hygroscopicity, moisture retention

CLC Number:

TQ35

Mengru ZHAN, Yanbin WANG, Shuhe KANG, Xingping LUO. Purification of Polysaccharides from Codonopsis pilosula by Macroporous Adsorption Resin and Its Whitening, Moisture Absorption and Moisturizing Properties[J]. Chemistry and Industry of Forest Products, 2023, 43(3): 79-88.

Figures/Tables 8

Table 1

Table 2

Fig.1

Table 3

Table 4

Fig.2

Fig.3

Fig.4

References 25

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成分ingredient	用量dosage/mL
成分ingredient	Z₁组group Z₁	Z₂组group Z₂	Z₃组group Z₃	Z₄组group Z₄
样品溶液sample solution	1	1	0	0
PBS	2	3	3	4
酪氨酸酶tyrosinase	1	0	1	0
L-酪氨酸/L-多巴L-tyrosine/L-dopa	1	1	1	1

树脂型号resin type	吸附率/% adsorption rate	解吸率/% desorption rate
H103	65.39±0.36	47.61±0.80
DM130	67.56±0.32	36.09±2.07
ADS-F8	72.99±0.09	54.81±1.28
HP-20	67.46±0.22	25.32±0.93
S-8	50.45±0.54	29.54±0.87
XAD-8	49.77±0.56	12.50±1.47
LSA-21	68.41±0.79	32.27±2.69
ADS-17	68.74±0.44	29.04±2.52
D101	60.67±1.39	37.40±0.86
X-5	62.58±0.87	37.90±1.56
NKA-9	67.99±0.75	22.75±1.54
AB-8	55.26±0.26	40.28±1.12
LSA-10	69.38±1.16	28.45±1.11
DA201	60.14±1.20	9.86±1.48

序号No.	X₁上样质量浓度/(g?L^-1)sample mass concentration	X₂洗脱液体积/mL effluent volume	X₃流速/(mL?h^-1)flow rate	综合评分(Y)/% comprehensive evaluation
1	1.5	200	100	44.91
2	1.0	150	100	75.31
3	1.5	100	100	54.02
4	1.0	100	150	48.21
5	0.5	100	100	52.92
6	1.0	150	100	72.02
7	1.5	150	50	56.86
8	1.0	150	100	69.65
9	1.5	150	150	48.92
10	0.5	150	50	54.3
11	0.5	200	100	49.61
12	1.0	200	150	47.00
13	1.0	150	100	74.26
14	1.0	100	50	51.00
15	1.0	200	50	52.65
16	0.5	150	150	51.38
17	1.0	150	100	72.93

方差来源soruces of variation	平方和quadratic sum	自由度degree of freedom	均方mean square	F值F-value	P值P-value	显著性significant
模型model	1 790.29	9	198.92	33.08	< 0.000 1	**
X₁	1.53	1	1.53	0.25	0.629 3
X₂	17.94	1	17.94	2.98	0.127 7
X₃	46.56	1	46.56	7.74	0.027 2	*
X₁X₂	8.41	1	8.41	1.40	0.275 6
X₁X₃	6.30	1	6.30	1.05	0.340 1
X₂X₃	2.04	1	2.04	0.34	0.578 1
X₁²	392.87	1	392.87	65.34	< 0.000 1	**
X₂²	690.88	1	690.88	114.90	< 0.000 1	**
X₃²	447.52	1	447.52	74.43	< 0.000 1	**
残差residual	42.09	7	6.01
失拟误差lack of fit	23.12	3	7.71	1.62	0.317 8
纯误差pure error	18.97	4	4.74
总和total	1 832.38	16

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Purification of Polysaccharides from Codonopsis pilosula by Macroporous Adsorption Resin and Its Whitening, Moisture Absorption and Moisturizing Properties

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