Abstract: Guaiacyl type dehydrogenation polymers(DHPs)were prepared from coniferin and coniferin (α-¹³C)by peroxidase,glucose oxidase and β glucosidase.The DHPs were analyzed by high resolution solid state carbon 13 nuclear magnetic resonance (¹³C NMR) spectroscopy and infrared spectroscopy.The result of IR indicated that the chemical structure of DHP was in well agreement with MWL prepared from ginkgo wood.It was proved that β-O-4,β-β,β-5 and coniferyl alcohol structures were the main substructures in DHPs although there were some minor substructures such as vanillin and α methylene units according to the determination of CP/MAS ¹³C NMR spectra.

Key words: dehydrogenation polymer, lignin, ¹³C isotopic tracer, solid state ¹³C NMR spectroscopy