Abstract: Polyprenols(C₇₅-C₁₁₀)of purity over 87.2% were separated and prepared from leaves of Gingkgo biloba L. Polyprenyl phosphate dichlorate was synthesized by choosing phosphorus oxychloride (POCl₃) as phosphate,and was converted into polyprenyl phosphate with triethanylamide as hydrolytic reagent with yield more than 65%.The molar ratio of polyprenols to POCl₃ was 1:5-10.Phosphate ester reaction temperature was below 10℃,and hydrolysis for 20 h at room temperature.The hydrolysate was purified by silicon gel chromatography and polyprenyl phosphate was obtained by HPLC,and its chemical structure was identified as polyprenyl monophosphate with IR,¹HNMR,¹³CNMR and HRMS.

Key words: leaves of Ginkgo biloba L., polyprenol, polyprenyl phosphate