香椿叶提取物清除DPPH自由基能力的测定方法

›› 2006, Vol. 26 ›› Issue (3): 69-72.

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Determining Method of DPPH Free Radical Scavenging Activity of Toona sinensis (A.Juss.) Roem. Leaves Extracts

CHEN Cong-jin^1,2, HUANG Ke-ying¹, LI De-liang¹, WANG Xu-qiang¹, YUAN Shuang-shan¹

1. Industry College, Central South University of Forestry and Technology, Zhuzhou 412006, China;2. Chemistry and Chemical Engineering College, Guangxi University, Nanning 530004, China

Received:2005-09-30 Revised:1900-01-01 Online:2006-09-30 Published:2006-09-30

Abstract

Abstract: A spectrophotometric method was established for determining 2,2-diphenyl-1-picrylhydrazyl (DPPH)free radical scavenging activity of natural antioxidants. The evaluating criterion of DPPH free radical scavenging activity was regarded as"IC₅₀" by determining the scavenging rate curves of rutin, quercetin,ascorbic acid and gallic acid. The DPPH free radical scavenging activity of extract from Toona sinensis (A.Juss.)Roem. leaves was expressed by IC₅₀. The condition of scavenging DPPH free radical of extracts from T. sinensis. leaves was determined by change of absorbency veriety at wavelength 517 nm after reacting DPPH with extracts. It was wavelength 517,reacting time 50 min. Under this condition, IC₅₀ of scavenging DPPH free radical of extracts from T. sinensis leaves is 22.026.

Key words: Toona sinensis (A.Juss.)Roem. leaves extracts, spectrophotometry, scavenging activity, 2-diphenyl-1-picrylhydrazyl

CLC Number:

TQ91
Q946

CHEN Cong-jin;HUANG Ke-ying;LI De-liang;WANG Xu-qiang;YUAN Shuang-shan. Determining Method of DPPH Free Radical Scavenging Activity of Toona sinensis (A.Juss.) Roem. Leaves Extracts[J]. , 2006, 26(3): 69-72.

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Determining Method of DPPH Free Radical Scavenging Activity of Toona sinensis (A.Juss.) Roem. Leaves Extracts

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