Abstract: For improving the yield of alkaloids from Corydalis yanhusuo W.T.Wang, response surface methodology was adopted to optimize the extraction process. The effects of different parameters on yield and purity of alkaloid were studied. Based on response surface methodology, the extraction conditions on the basis of single factor experiment using alkaloid yield as index were optimized. The optimal conditions were: extraction time 116 min, extraction temperature 49℃, 80% (volume fraction) EtOH as solvent, EtOH dose 10mL/g (based on raw material). Under optimal conditions, the yield of alkaloids and purity were 0.598% and 17.38% respectively, after three times extraction.

Key words: Corydalis yanhusuo W.T.Wang, alkaloids, response surface, extraction