Abstract: Immobilized cells were prepared through embedment of Trichoderma aureoviride with calcium alginate. The suitable culture conditions were sodium alginate 50 g/L, initial pH value of the substrate 4.0-4.5 and using filter paper pulp as carbon source of the substrate. The enzymatic activity of the immobilized cells was improved when concentration of calcium dichloride was 20 g/L, initial pH value of the substrate was 4.5, concentration of sodium alginate was 50 g/L using filter paper pulp as carbon source of the substrate. Addition of the surfactant Twin-80 could further increase enzymatic activity. Three endoglucanases and two β-glucosidases from the fungi were separated and purified to electrophoretic homogeneity through processes of (NH₄)₂SO₄ fractional precipitation, Sephadex G-100 gel chromatography and DEAE Sephadex A-50 ion exchange chromatography. They were designated as EGⅠ, EGⅡ, EGⅢ, BGⅠand BGⅡ, with relative molecular weights (M_[HT6SS]r[HT5"SS])of 62 300, 71 900, 52 600, 85 300 and 78 300 by SDS-PAGE, and isoelectric points of 5.4, 4.8, 5.0, 5.6 and 5.8, respectively.

Key words: cellulase, Trichoderma aureoviride, immobilization, isoelectric point