HPLC法测定虎杖白藜芦醇的含量及其稳定性研究

›› 2004, Vol. 24 ›› Issue (2): 61-64.

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HPLC DETERMINATION OF RESVERATROL CONTENT IN POLYGONUM CUSPIDATUM AND THE STABILITY OF RESVERATROL

CAO Yong^1,2, YU Hua-zhong², ZHANG Min², LI Guo-zhang², XIAO Lang-tao¹

1. Hunan Provincial Key Laboratory of Phytohormones, Hunan Agriculture University, Changsha 410128, China;2. Key laboratory of Hunan Forest Product & Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China

Received:2003-09-28 Revised:1900-01-01 Online:2004-06-30 Published:2004-06-30

Abstract

Abstract: Resveratrol is one of the most feasible medicine for preventing human cancer.It is unstable against light and heat.An extracting method was established in this study,which is simple,and of high yield.Study on the conditions of high performance liquid chromatography for the determination of resveratrol content was investigated to be as follow:Shim-park CLC-ODS column(150 mm×6.0 mm,i.d.5 μm),mobil phase CH₃CH₂CN:H₂O(14:59),flow rate 1.0 mL/min,wavelength 306 nm.The lineal correlation between content of resveratrol and peak area is good.The lower detection limit was 10 ng/mL.

Key words: Polygonum cuspidatum, resveratrol, HPLC

CLC Number:

TQ91
S789

CAO Yong;YU Hua-zhong;ZHANG Min;LI Guo-zhang;XIAO Lang-tao. HPLC DETERMINATION OF RESVERATROL CONTENT IN POLYGONUM CUSPIDATUM AND THE STABILITY OF RESVERATROL[J]. , 2004, 24(2): 61-64.

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HPLC DETERMINATION OF RESVERATROL CONTENT IN POLYGONUM CUSPIDATUM AND THE STABILITY OF RESVERATROL

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