刺玫果黄酮对胰岛素抵抗HepG2细胞糖代谢功能研究

doi:10.3969/j.issn.0253-2417.2023.03.006

Abstract

Abstract:

To study the effects of flavonoids from Rosa davurica Pall. on glucose metabolism and key enzyme activities in insulin-resistant HepG(IR-HepG2) cells, the HepG2 cells were induced by high concentration of insulin to established IR-HepG2 cell model. The cells were treated with R. davurica flavonoid for 24 h. The cell activity was detected by MTT method, and the effect of the glucose consumption treated by R. davurica flavonoid was evaluated. The intracellular glycogen accumulation levels and IR-HEPG2 cell key enzymes hexokinase(HK), pyruvate kinase(PK), glucose-6-phosphatase(G6Pase), phosphoenol acetate carboxylase(PEPCK) activities were determined. The results showed that IR-HepG2 cell model was successfully established by treating HepG2 cells with 0.1 μmol/L insulin for 24 h. The glucose consumption rate of cells in the 0.10-1.00 g/L R. davurica flavonoid treatment group was all more than 18%, and the cell activity was best after cultured for 24 h. Compared with the model group, the 1.00 g/L R. davurica flavonoid treatment group increased the glycogen content by 81.45%(P < 0.05), HK and PK viability increased by 29.26%(P < 0.05) and 31.93%(P < 0.05), and the activities of PEPCK and G6Pase decreased by 30.82%(P < 0.05) and 7.32%(P < 0.05), respectively. R. davurica flavonoids were potential bioactive compounds that could regulate carbohydrate metabolism by modulating key enzymes in IR-HepG2 cells warrants in-depth study.

Key words: Rosa davurica Pall. flavonoids, HepG2 cell, insulin resistance, glucose consumption, key enzyme

CLC Number:

TQ35

Xiaojie WU, Bin KUAI, Haitao ZHAO, Tongshuai SHI, Junmeng QIU, Qun FU. Flavonoids from Rosa davurica Pall. on Glucose Metabolism Function in Insulin Resistant HepG2 Cells[J]. Chemistry and Industry of Forest Products, 2023, 43(3): 41-48.

Figures/Tables 4

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质量浓度/(g·L^-1)mass concentration	OD值OD value	细胞存活率¹⁾/%cell viability
空白对照blank control	0.593±0.033	100.00±4.95^ab
0.01	0.609±0.026	102.73±3.94^a
0.05	0.587±0.024	98.95±3.62^ab
0.10	0.623±0.038	105.06±5.79^a
0.50	0.616±0.038	103.81±5.78^a
1.00	0.558±0.018	94.13±2.78^b
1.50	0.446±0.023	75.21±3.50^c
2.00	0.348±0.018	58.62±2.61^d
3.00	0.299±0.024	50.49±3.67^e
4.00	0.281±0.019	47.35±2.82^e

胰岛素浓度/(μmol·L^-1)insulin concentration	葡萄糖消耗量/(mmol·L^-1)glucose consumption	细胞存活率/%cell viability
0	4.127±0.082^c	100.00±2.049^c
0.001	3.951±0.121^c	98.305±5.532^c
0.01	3.967±0.112^c	106.163±9.143^b
0.1	2.980±0.049^e	115.408±10.431^a
1	3.887±0.021^d	115.023±7.859^a
10	4.350±0.013^b	106.086±4.546^b
100	4.622±0.093^a	113.559±5.983^ab

分组group	葡萄糖消耗量glucose consumption/(mmol·L^-1)			细胞存活率cell viability/%
分组group	12 h	24 h	36 h	12 h	24 h	36 h
空白组blank	4.120±0.069^a	4.270±0.130^a	4.590±0.109^a	100.000±3.240^c	100.130±1.200^b	100.000±0.182^ab
模型组model	1.288±0.095^f	2.823±0.072^d	2.567±0.021^e	98.581±1.460^cd	106.480±0.841^a	103.771±1.191^a
阳性对照组positive control	3.554±0.136^b	4.215±0.115^ab	4.347±0.101^b	103.990±3.351^b	101.051±2.023^b	105.901±3.30^a
0.01 g/L	1.963±0.180^e	3.747±0.096^c	3.924±0.104^d	109.811±3.851^a	99.671±2.613^bc	102.821±3.230^a
0.05 g/L	2.043±0.015^e	3.783±0.142^c	3.881±0.101^d	95.642±2.443^de	99.670±3.561^bc	92.700±5.351^cd
0.10 g/L	2.491±0.062^d	4.059±0.110^b	4.051±0.083^c	96.730±1.070^cd	98.363±2.200^bc	93.341±6.301^cd
0.50 g/L	2.471±0.071^d	4.155±0.137^ab	4.131±0.172^c	92.044±2.932^e	96.895±3.530^c	95.710±3.300^bc
1.00 g/L	2.707±0.151^c	4.187±0.035^ab	4.259±0.039^b	92.261±2.910^e	98.202±2.311^bc	88.201±0.603^d

分组group	糖原质量分数/(g·g^-1)gycogen content	HK活性/(U·g^-1)HK vitality	PK活性/(U·g^-1)HK vitality	PEPCK活性/(U·L^-1)PEPCK vitality	G6Pase活性/(U·L^-1)G6Pase vitality
空白组blank	0.787±0.021^a	18.805±1.560^a	145.915±6.294^a	3.367±0.084^d	86.474±5.324^c
模型组model	0.372±0.011^d	13.285±2.488^c	100.719±3.008^d	7.209±0.463^a	98.228±8.394^a
阳性对照组positive control	0.690±0.015^b	17.952±1.498^a	134.082±3.807^b	3.782±0.282^d	89.105±6.903^bc
0.10 g/L	0.514±0.015^c	15.742±1.487^bc	119.635±5.438^c	6.794±0.444^a	94.368±1.898^ab
0.50 g/L	0.520±0.008^c	16.705±1.410^ab	124.463±6.554^bc	5.897±0.563^b	93.140±2.373^bc
1.00 g/L	0.675±0.014^b	17.172±0.859^ab	132.877±8.305^b	4.987±0.322^c	91.035±4.776^bc

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Flavonoids from Rosa davurica Pall. on Glucose Metabolism Function in Insulin Resistant HepG2 Cells

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