纤维素前驱物6-13C标记示踪研究纤维素与木质素连接方式

doi:10.3969/j.issn.0253-2417.2014.01.007

林产化学与工业 ›› 2014, Vol. 34 ›› Issue (1): 37-42.doi: 10.3969/j.issn.0253-2417.2014.01.007

纤维素前驱物6-¹³C标记示踪研究纤维素与木质素连接方式

向松明, 谢益民, 杨海涛, 姚兰

湖北工业大学制浆造纸工程学院, 湖北武汉 430068

收稿日期:2013-05-02 出版日期:2014-02-25 发布日期:2014-03-05
通讯作者: 谢益民，教授，博士生导师，主要从事木质素研究工作；E-mail：ppymxie@scut.edu.cn。 E-mail:ppymxie@scut.edu.cn
作者简介:向松明（1988-），女，湖北松滋人，硕士生，主要从事植物纤维化学结构研究工作；E-mail：Mingjia0106@163.com
基金资助:
国家自然科学基金资助项目（31070521）

Analysis of the Association between Cellulose and Lignin by Carbon 13 Tracer Method with Cellulose Precursor

XIANG Song-ming, XIE Yi-min, YANG Hai-tao, YAO Lan

School of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, China

Received:2013-05-02 Online:2014-02-25 Published:2014-03-05

摘要/Abstract

摘要： 从细胞壁多糖的角度分析糖单元与木质素苯丙烷结构单元之间的共价键连接方式，合成了带6-¹³C 标记的纤维素前驱物尿苷二磷酸葡萄糖，并将其与苯丙氨酸解氨酶（PAL）的抑制剂L-2-氨氧基-3-苯基丙酸（AOPP）及外源性木质素前驱物松柏醇-β-D-葡萄糖苷一起投入生长中的银杏植物体内，分析结果证明了（6-¹³C）尿苷二磷酸葡萄糖是对植物中纤维素进行选择性¹³C 标记的合适的前驱物。从正常生长的银杏新生木质部组织提取木质素-碳水化合物复合体（LCC），并用纤维素酶和半纤维素酶酶解LCC得LCC酶解产物（EDLCC）。红外光谱及 ¹³C NMR 检测LCC和EDLCC，分析证实了纤维素6位碳与木质素以苯甲醚键方式连接。

关键词: 纤维素, ¹³C标记, ¹³C NMR, 木质素-碳水化合物复合体, 苯甲醚键

Abstract: In this paper, carbohydrate part of the lignin-carbohydrate complexes(LCC) in cell wall was labelled with ¹³C and traced with ¹³C NMR. Cellulose precursor, i.e., uridine diphoshphate glucose-[6-¹³C], was synthesized, and injected into a living ginkgo tree together with lignin inhibitor AOPP and exogenous lignin precursor. It is proved that uridine diphoshphate glucose-[6-¹³C] is a suitable precursor for selective ¹³C-enrichment of cellulose in a plant. The specifically ¹³C-enriched LCC was isolated from the newly formed xylem of ginkgo shoots. Then it was degraded by cellulase and hemicellulase. Thus the lignin-rich fractions,which were called enzyme-degraded LCC (EDLCC), were obtained. By determining their FT-IR and ¹³C NMR spectra, information confirms that the bonds between C₆ position of glucose units in cellulose and α-carbons of lignin side chain are benzyl ether linkage.

Key words: cellulose, ¹³C isotopic tracer, ¹³C NMR, lignin-carbohydrate complexes, benzyl ether linkage

中图分类号:

TQ35

向松明, 谢益民, 杨海涛, 姚兰. 纤维素前驱物6-¹³C标记示踪研究纤维素与木质素连接方式[J]. 林产化学与工业, 2014, 34(1): 37-42.

XIANG Song-ming, XIE Yi-min, YANG Hai-tao, YAO Lan. Analysis of the Association between Cellulose and Lignin by Carbon 13 Tracer Method with Cellulose Precursor[J]. Chemistry and Industry of Forest Products, 2014, 34(1): 37-42.

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纤维素前驱物6-¹³C标记示踪研究纤维素与木质素连接方式

Analysis of the Association between Cellulose and Lignin by Carbon 13 Tracer Method with Cellulose Precursor

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