桉木热磨与高温热水协同预处理对酶解效果的影响及工艺优化

doi:10.3969/j.issn.0253-2417.2014.04.006

林产化学与工业 ›› 2014, Vol. 34 ›› Issue (4): 30-36.doi: 10.3969/j.issn.0253-2417.2014.04.006

桉木热磨与高温热水协同预处理对酶解效果的影响及工艺优化

徐绍华, 武书彬, 李霄虹

华南理工大学制浆造纸工程国家重点实验室, 广东广州 510640

收稿日期:2013-05-28 出版日期:2014-08-25 发布日期:2015-08-18
作者简介:徐绍华（1990-），男，安徽淮北人，硕士生，研究方向为生物质的高品质转化与利用；E-mail：865941863@qq.com
基金资助:
国家863计划资助（2012AA101806）；国家自然科学基金项目资助（31270635）；广东省科技计划项目（2011A090200006）

Effects of Combination of Thermo-mechanical and Liquid Hot Water Pretreatment on the Efficiency of the Enzymatic Hydrolysis of Eucalyptus and Its Process Optimization

XU Shao-hua, WU Shu-bin, LI Xiao-hong

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China

Received:2013-05-28 Online:2014-08-25 Published:2015-08-18
Contact: 武书彬（1965-），男，教授，博士生导师，主要从事植物纤维类生物质化学结构、植物纤维类生物质转化为清洁能源和化工原料等方面的研究；E-mail：shubinwu@scut.edu.cn。 E-mail:shubinwu@scut.edu.cn

摘要/Abstract

摘要： 为提高桉木原料的酶解糖化效果，降低原料处理成本，采用热磨与高温热水法联合对桉木原料进行预处理，并对高温热水预处理后样品进行纤维素酶酶解糖化。通过对预处理液和酶解液中木糖和葡萄糖得率的测定，来研究预处理条件对糖得率的影响。以预处理液和酶解液中的总糖得率作为预处理工艺条件的评价指标，得到最优的预处理条件为：预处理温度 180℃、保温时间 40 min、固液比1：20（g：mL）。在此条件下，桉木热磨后原料经预处理、酶解后总木糖得率为 11.78%，木糖的转化率为 82.67%；总葡萄糖得率为 36.33%，葡萄糖的转化率为 78.90%。采用傅立叶红外光谱（FT-IR）、扫描电镜（SEM）等分析预处理样的理化特性，结果表明：桉木原料热磨后颗粒变小，呈丝状，纤维形态和表面结构基本不变；而高温热水预处理后物料的纤维结构松散、碎化，断裂明显，改善了纤维素酶的可及度，提高了酶解效率。

关键词: 桉木, 热磨, 高温热水预处理, 酶解, FT-IR, SEM

Abstract: Combination of thermo-mechanical pulping (TMP) process and liquid hot water pretreatment method had been adopted to improve the enzymatic saccharification efficiency and reduce the processing costs of eucalyptus. The pretreated sample was then hydrolyzed by cellulase. The pretreatment conditions on the influence of sugars yield had been discussed by measuring the yield of xylose and glucose in the pretreated hydrolyzate and enzymatic hydrolyzate. The optimal pretreatment condition was 180℃(cooking temperature), 40 min(residence time) and 5%(substrate concentration). It was obtained by using the total sugars in the pretreated hydrolyzate and enzymatic hydrolyzate as the evaluation index. Under this pretreatment condition, the conversion of xylose and glucose reached 82.70% and 78.90%, respectively. The physics and chemical properties of pretreated residue were characterized using FT-IR, SEM so as to analyse effect of two pretreatment methods. The results showed that the particle of raw eucalyptus was diminished, the shape was filiform, but the fibre morphology was maintained. However, the structure of fibre was loose and smashed after the hot water pretreatment, and this improved the accessibility of cellulase and efficiency of enzymolysis.

Key words: eucalyptus, TMP, hot water pretreatment, enzyme hydrolysis, FT-IR, SEM

中图分类号:

TQ35

徐绍华, 武书彬, 李霄虹. 桉木热磨与高温热水协同预处理对酶解效果的影响及工艺优化[J]. 林产化学与工业, 2014, 34(4): 30-36.

XU Shao-hua, WU Shu-bin, LI Xiao-hong. Effects of Combination of Thermo-mechanical and Liquid Hot Water Pretreatment on the Efficiency of the Enzymatic Hydrolysis of Eucalyptus and Its Process Optimization[J]. Chemistry and Industry of Forest Products, 2014, 34(4): 30-36.

参考文献

[1] KUMAR R,SINGH S,SINGH O V.Bioconversion of lignocellulosic biomass:Biochemical and molecular perspectives[J].Journal of Industrial Microbiology and Biotechnology,2008,35(5):377-391.
[2] ADSUL M G,GHULE J E,SHAIKH H,et al.Enzymatic hydrolysis of delignified bagasse polysaccharides[J].Carbohydrate Polymers,2005,62(1):6-10.
[3] PAN Xue-jun,ARATO C,GILKES N,et al.Biorefining of softwoods using ethanol organosolv pulping:Preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products[J].Biotechnology and Bioengineering,2005,90(4):473-481.
[4] SINGH R,VARMA A J,LAXMAN R S,et al.Hydrolysis of cellulose derived from steam exploded bagasse by Penicillium cellulases:Comparison with commercial cellulase[J].Bioresource Technology,2009,100(23):6679-6681.
[5] KO J K,BAK J S,JUNG M W,et al.Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes[J].Bioresource Technology,2009,100(19):4374-4380.
[6] MORINELLY J E,JENSEN J R,BROWNE M,et al.Kinetic characterization of xylose monomer and oligomer concentrations during dilute acid pretreatment of lignocellulosic biomass from forests and switchgrass[J].Industrial and Engineering Chemistry Research,2009,48(22):9877-9884.
[7] KOBAYASHI N,OKADA N,HIRAKAWA A,et al.Characteristics of solid residues obtained from hot-compressed-water treatment of woody biomass[J].Industrial and Engineering Chemistry Research,2009,48(1):373-379.
[8] DIEN B S,LI X L,ITEN L B,et al.Enzymatic saccharification of hot-water pretreated corn fiber for production of monosaccharides[J].Enzyme and Microbial Technology,2006,39(5):1137-1144.
[9] 屈维均.制浆造纸实验[M].北京:中国轻工业出版社,1990:34-51.
[10] 武玉波,冯秀燕.木聚糖酶酶活测定方法的研究[J].中国饲料,2008(4):34-36.
[11] 仉磊,李涛,王磊,等.糠醛渣的纤维素酶水解及其最优纤维素转化条件[J].农业工程学报,2009,25(10):226-230.
[12] YU Qiang,ZHUANG Xin-shu,YUAN Zhen-hong,et al.Two-step liquid hot water pretreatment of Eucalyptus grandis to enhance sugar recovery and enzymatic digestibility of cellulose[J].Bioresource Technology,2010,101(13):4895-4899.
[13] 荆琪.高温液态水中单糖分解反应动力学研究[D].杭州:浙江大学博士学位论文,2007.
[14] PALMQVIST E,HAHN-HÄGERDAL B.Fermentation of lignocellulosic hydrolysates.II:Inhibitors and mechanisms of inhibition[J].Bioresource Technology,2000,74(1):25-33.
[15] SUN Ye,CHENG Jia-yang.Hydrolysis of lignocellulosic materials for ethanol production:A review[J].Bioresource Technology,2002,83(1):1-11.
[16] WEI Wei-qi,WU Shu-bin,LIU Li-guo.Combination of liquid hot water pretreatment and wet disk milling to improve the efficiency of the enzymatic hydrolysis of eucalyptus[J].Bioresource Technology,2013,128:725-730.
[17] SONG An-dong,WANG Lei,WANG Feng-qin,et al.Influence of microbial treatment methods on the structure of rice straw[J].Chinese Journal of Bioprocess Engineering,2009,7(4):72-76.

桉木热磨与高温热水协同预处理对酶解效果的影响及工艺优化

Effects of Combination of Thermo-mechanical and Liquid Hot Water Pretreatment on the Efficiency of the Enzymatic Hydrolysis of Eucalyptus and Its Process Optimization

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