辐照预处理水稻秸秆在高底物浓度下的酶解

doi:10.3969/j.issn.0253-2417.2015.02.020

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (2): 129-134.doi: 10.3969/j.issn.0253-2417.2015.02.020

辐照预处理水稻秸秆在高底物浓度下的酶解

陈亮¹, 苏小军², 陈静萍¹, 武小芬¹, 熊兴耀^2,3, 胡秋龙², 王克勤¹

1. 湖南省核农学与航天育种研究所, 湖南长沙 410125;
2. 湖南农业大学湖南省作物种质创新与资源利用重点实验室, 湖南长沙 410128;
3. 中国农业科学院蔬菜花卉研究所, 北京 100081

收稿日期:2014-05-13 出版日期:2015-04-25 发布日期:2015-04-24
通讯作者: 王克勤(1964-),男,研究员,博士,主要从事核技术在生物质能源中的应用研究;E-mail:wkq6412@163.com。 E-mail:wkq6412@163.com
作者简介:陈亮(1981-),男,助理研究员,硕士,主要从事核技术在生物质能源中的应用研究;E-mail:chenliang912@163.com
基金资助:
国家863计划资助项目(2012AA101804); 湖南省农业科学院科技创新专项(无编号)

Enzymatic Hydrolysis of Rice Straw Pretreated by Irradiation at High Solid Loading

CHEN Liang¹, SU Xiao-jun², CHEN Jing-ping¹, WU Xiao-fen¹, XIONG Xing-yao^2,3, HU Qiu-long², WANG Ke-qin¹

1. Institute of Nuclear Agricultural Science & Space Mutation Breeding, Changsha 410125, China;
2. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China;
3. The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2014-05-13 Online:2015-04-25 Published:2015-04-24

摘要/Abstract

摘要： 研究了高剂量辐照对水稻秸秆聚糖组分的影响,探讨了采用分批补料提高辐照预处理水稻秸秆酶解体系底物浓度以获得高浓度糖的可行性。结果表明:水稻秸秆经高剂量辐照后其聚糖含量下降,水溶性总糖由0.7%增至13.4%;采用分批补料的方法,能使酶解体系最终底物质量分数达到40%,在32.8 FPU/g(以纤维素质量计)的酶添加量条件下,可获得酶解葡萄糖质量浓度达104.6 g/L,纤维素酶解转化率可达到77.1%。

关键词: 辐照, 水稻秸秆, 高底物浓度, 酶解

Abstract: The effect of high dose irradiation on polysaccharides of rice straw was studied. The feasibility of the achievement of high-concentration sugar by the way of improving substrate concentration through fed-batch enzymatic hydrolysis process of irradiated rice straw, was then investigated. The result showed that the polysaccharides content of irradiated rice straw decreased and the water-soluble sugar increased from 0.7% to 13.4%. By the way of fed-batch enzymatic hydrolysis, the solid loading could reach 40% (mass fraction) in the enzymatic hydrolysis system. And 104.6 g/L glucose concentration as well as 77.1% cellulose conversion were obtained by this process under the condition of 32.8 FUP/g (calculated by cellulose) cellulase loading.

Key words: irradiation, rice straw, high solid loading, enzymatic hydrolysis

中图分类号:

TQ35

陈亮, 苏小军, 陈静萍, 武小芬, 熊兴耀, 胡秋龙, 王克勤. 辐照预处理水稻秸秆在高底物浓度下的酶解[J]. 林产化学与工业, 2015, 35(2): 129-134.

CHEN Liang, SU Xiao-jun, CHEN Jing-ping, WU Xiao-fen, XIONG Xing-yao, HU Qiu-long, WANG Ke-qin. Enzymatic Hydrolysis of Rice Straw Pretreated by Irradiation at High Solid Loading[J]. Chemistry and Industry of Forest Products, 2015, 35(2): 129-134.

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辐照预处理水稻秸秆在高底物浓度下的酶解

Enzymatic Hydrolysis of Rice Straw Pretreated by Irradiation at High Solid Loading

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