O/W型山苍子精油微乳的制备及其性能研究

doi:10.3969/j.issn.0253-2417.2021.01.010

摘要/Abstract

摘要：

通过加水法制备山苍子精油微乳，单因素试验考察了不同的表面活性剂、助表面活性剂、表面活性剂与助表面活性剂的质量比（K_m）以及制备温度对山苍子精油微乳形成的影响，再通过正交试验，得到水包油（O/W）型山苍子精油微乳的最佳制备条件为：曲拉通X-100（Triton X-100）为表面活性剂，无水乙醇为助表面活性剂，K_m值为1，制备温度为25℃。以最佳制备条件中混合表面活性剂与山苍子精油的质量比为9：1，含水率为70%制得标样1，微乳的平均粒径、多分散系数（PDI）、电导率和pH值分别为13.43 nm、0.125、83 mS/m和5.82。对标样1进行性能测试分析，发现其在1 000~4 000 r/min的速率下进行离心时，微乳外观无变化，可证明其离心稳定性较好；在标样1和精油中加入等量的亚甲基蓝水溶液，亚甲基蓝水溶液在标样1中的扩散速度显著快于在精油中的，可证明微乳的水溶性显著优于精油；在缓释12 h后，微乳中缓释的柠檬醛含量显著少于精油中缓释的，说明微乳的缓释性优于精油；在10~30 g/L的质量浓度范围内，山苍子精油微乳液标样1比精油表现出更高的1，1-二苯基-2-苦肼基自由基（DPPH·）和2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐自由基（·ABTS⁺）清除率，质量浓度30 g/L下山苍子精油微乳液标样1的DPPH·和·ABTS⁺清除率为95%和54.7%，而山苍子精油的DPPH·和·ABTS⁺清除率仅为44.79%和16.4%，说明将山苍子精油制备成微乳可以显著提高其抗氧化性，且微乳对DPPH·的抗氧化性显著优于对·ABTS⁺的。

关键词: 山苍子精油, 微乳, 制备条件, 抗氧化性

Abstract:

A microemulsion of Litsea cubeba(Lour.) Pers. oil was prepared by adding water. The influence of different surfactant and cosurfactant-types, the mass ratio of surfactant to cosurfactant(K_m), and preparation temperature on the formation of water-in-oil(O/W) type microemulsion of L. cubeba oil were investigated using a single factor method, and through orthogonal experiments, the optimum preparation process of O/W type L. cubeba oil microemulsion was determined as follows: Triton X-100 as surfactant, absolute alcohol as cosurfactant, K_mvalue was equal to 1, and preparation temperature was set to 25 ℃. Under the best preparation conditions, the ratio of the mixed surfactant and the essential oil of L. cubeba was 9∶1, and the water content was 70% for sample 1. The average particle diameter, polydispersion coefficient, conductivity and pH value of sample 1 were 13.43 nm, 0.125, 83 mS/m, and 5.82, respectively. The performance test and analysis of sample 1 showed that there was no change in the appearance of the microemulsion when it was centrifuged at the rate of 1 000 to 4 000 r/min, which could prove that the centrifugal stability was better than essential oil. Adding the same amount of methylene blue aqueous solution to sample 1 and essential oil, its diffusion rate in sample 1 was significantly faster than that in the essential oil, which proved that the water solubility of microemulsion was significantly better than that of the essential oil.After 12 h of sustained-release, the content of sustained-release citral in the microemulsion was significantly less than that of the sustained-release citral in the essential oil, indicating that the sustained release of the microemulsion was better than that of the essential oil itself. The results of 1, 1-diphenyl-2-picrylhydrazyl free radical(DPPH·) and 2, 2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical(·ABTS⁺) scavenging experiments indicated that DPPH· and·ABTS⁺ scavenging rates of sample 1 of L. cubeba essential oil microemulsion were significantly higher than those of essential oil in the concentration range of 10-30 g/L. The DPPH·and·ABTS⁺ scavenging rates of the L. cubeba essential oil microemulsion sample 1 at 30 g/L concentration were 95% and 54.7%, while those of L. cubeba essential oil were only 44.79% and 16.4%, respectively, indicating that L. cubeba essential oil prepared as microemulsion could significantly improve its antioxidant property, and the anti-oxidation property of microemulsion to DPPH· was significantly better than that of·ABTS⁺.

Key words: Litsea cubeba essential oil, microemulsion, preparation conditions, antioxidant activity

中图分类号:

TQ35
TS221

程晨, 司红燕, 王宗德, 廖圣良, 邹晨阳, 王鹏. O/W型山苍子精油微乳的制备及其性能研究[J]. 林产化学与工业, 2021, 41(1): 67-76.

Chen CHENG, Hongyan SI, Zongde WANG, Shengliang LIAO, Chenyang ZOU, Peng WANG. Preparation Conditions and Properties of O/W Type Litsea cubeba Oil Microemulsion[J]. Chemistry and Industry of Forest Products, 2021, 41(1): 67-76.

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序号 No.	助表面活性剂 cosurfactant	表面活性剂 surfactant	K_m值 K_m value	制备温度/℃ preparation temperature	微乳区面积/总面积 microemulsion area/total area
1	无水乙醇absolut alcohol	Triton X-100	1	15	163.19/300
2	无水乙醇absolut alcohol	EL-30	2	25	106.18/300
3	无水乙醇absolut alcohol	EL-35	3	35	77.66/300
4	PEG-400	Triton X-100	2	35	19.01/300
5	PEG-400	EL-30	3	15	0.14/300
6	PEG-400	EL-35	1	25	0.07/300
7	1, 2丙二醇1, 2-propanediol	Triton X-100	3	25	129.99/300
8	1, 2丙二醇1, 2-propanediol	EL-30	1	35	95.28/300
9	1, 2丙二醇1, 2-propanediol	EL-35	2	15	39.47/300
k₁	115.68	104.06	86.18	67.60
k₂	6.41	67.20	54.89	78.75
k₃	88.25	39.07	69.26	63.98
R	109.27	64.99	31.29	14.77

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