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林产化学与工业

林产化学与工业 ›› 2003, Vol. 23 ›› Issue (04): 54-58.

• 研究报告 • 上一篇    下一篇

百合总皂苷定量测定方法的研究

吉宏武1, 丁霄霖2   

  1. 1. 湛江海洋大学 食品科学工程系, 江苏 无锡 214036;2. 江南大学 食品学院, 广东 湛江 524025
  • 收稿日期:2003-01-22 修回日期:1900-01-01 出版日期:2003-12-30 发布日期:2003-12-30

QUANTITATIVE DETERMINATION OF SAPONINS FROM BULBS OF LILY

JI Hong-wu1, DING Xiao-lin2   

  1. 1. Department of Food Science and Technology, Zhanjiang Ocean University, Zhanjiang 524025, China;2. School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China
  • Received:2003-01-22 Revised:1900-01-01 Online:2003-12-30 Published:2003-12-30

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摘要： 应用比色法研究了百合(Lilium brownii var.colchesteri)总皂苷的定量测定方法,结果表明:以甲醇为提取剂,采用超声波提取1h,然后以等体积的水饱和正丁醇萃取4次制备样品,按高氯酸5mL、时间20min、温度55℃的条件进行显色,根据其406nm处的吸光度和回归方程进行百合皂苷的定量测定,该方法具有简单、干扰小、准确度高、分析速度快等优点,适合于大量试样的分析。

关键词: 百合皂苷, 超声波提取

Abstract: A quantitative analytical method of lily(Lilium brownii var.colchesteri) saponins was studied by colorimetry.Results show that lily saponins can be well calculated according to their colorimetric absorption at 406 nm by using (ultrasonic) treatment of the sample in methanol for 1 h, and extracting for 4 times with n-butanol(1:1, volume), applying HClO4(5 mL), 20 min at 55℃ for colorimetric reaction.The method established has less interference, good precision, simplicity, rapidity, and can be well adopted for determination of saponins in a great deal of lily samples.

Key words: saponins of Lilium brownii var.colchesteri, ultrasonic extraction

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