杜仲叶提取绿原酸的中间试验研究

林产化学与工业 ›› 2003, Vol. 23 ›› Issue (3): 73-76.

杜仲叶提取绿原酸的中间试验研究

马希汉, 尉芹, 景谦平

西北农林科技大学生命科学学院, 陕西杨陵 712100

收稿日期:2003-01-20 修回日期:1900-01-01 出版日期:2003-09-30 发布日期:2003-09-30

STUDY ON TECHNIQUE UPON ISOLATION AND PURIFICATION OF CHLOROGENIC ACID FROM LEAVES OF EUCOMMIA

MA Xi-han, WEI Qin, JING Qian-ping

College of Life Sciences, Northwest Sci Tech University of Agricultureand Forestry, Yangling 712100, China

Received:2003-01-20 Revised:1900-01-01 Online:2003-09-30 Published:2003-09-30

摘要/Abstract

摘要： 本研究在以前研究的基础上进行中间试验,利用以下工艺流程从杜仲叶中制得绿原酸纯品:40%乙醇提取(80℃,2h,1:16料液比,提取2次)^→制备浸膏(真空浓缩,80℃鼓风干燥)^→加水转溶(4次)^→铅盐沉淀(每100g杜仲叶用140g乙酸铅)^→解析绿原酸(20%H2SO4)^→乙酸乙酯萃取(约20次)^→浓缩析出粗品^→重结晶得纯品,平均得率0.53%,纯度98.35%。研究中还发现杜仲叶中不含异绿原酸。

关键词: 绿原酸, 杜仲叶

Abstract: Based on the previous study, chlorogenic acid was isolated and purified from leaves of Eucommia ulmoides Oliver in an pilot scale experiment by the following process:refluxing by 40% ethanol(80℃, 2 h, ratio of material to solvent:1:16, extract twice)^→extracts preparation(vaccum evaperation, drying at 80℃)^→extraction by water(4 times)^→precipitation by Pb(OAc) 2\^→resolution of chlorogenic acid by 20% sulfuric acid^→extraction by ethyl acetate(20 times)^→condensation^→re crystallization.The yield of chlorogenic acid is 0.53%, at purity 98.35%.Results of the experiment showed that this technological process was feasible and practical.It was found that no isomers of chlorogenic acid occurs in the leaves of E.ulmoides after comparing with honeysuckle flowers and raw coffee beans, in which the occurrence of iso-chlorogenic acids was reported.This is traceable to the views of secondary metabolism and chemical reaction.

Key words: chlorogenic acid, leaves of Eucommia ulmoides Oliver

中图分类号:

马希汉;尉芹;景谦平. 杜仲叶提取绿原酸的中间试验研究[J]. 林产化学与工业, 2003, 23(3): 73-76.

MA Xi-han;WEI Qin;JING Qian-ping . STUDY ON TECHNIQUE UPON ISOLATION AND PURIFICATION OF CHLOROGENIC ACID FROM LEAVES OF EUCOMMIA[J]. , 2003, 23(3): 73-76.

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