黄檗中生物碱含量的高效液相色谱分析

林产化学与工业 ›› 2004, Vol. 24 ›› Issue (S1): 115-118.

黄檗中生物碱含量的高效液相色谱分析

秦彦杰¹, 张玉红², 王洋¹, 阎秀峰¹

1. 东北林业大学生命科学学院, 黑龙江哈尔滨 150040;2. 东北林业大学森林植物生态学教育部重点实验室, 黑龙江哈尔滨 150040

收稿日期:2003-12-25 修回日期:1900-01-01 出版日期:2004-10-20 发布日期:2004-10-20

DETERMINATION OF ALKALOIDS CONTENT IN PHELLODENDRON AM URENSE RUPR. BY HPLC

QIN Yan-jie¹, ZHANG Yu-hong², WANG Yang¹, YAN Xiu-feng¹

1. College of Life Sciences, Northeast Forestry University, Harbin 150040, China;2. Key Laboratory of Plant Forestry Ecology, Northeast Forestry University, Harbin 150040, China

Received:2003-12-25 Revised:1900-01-01 Online:2004-10-20 Published:2004-10-20

摘要/Abstract

摘要： 建立了一种简便、快速、准确的测定黄檗(Phellodendron amurense Rupr.)中生物碱含量的高效液相色谱分析方法。色谱条件:采用日本KYA HIQ silC 18柱(250mm×4.6mm,5μm),流动相乙腈/水溶液(1000mL溶液中含磷酸二氢钾3.4g及十二烷基磺酸钠1.7g)1:1,流速1.0mL/min,检测波长345nm,进样量10μL。样品制备以63%的乙醇作溶剂,在41℃下超声波提取黄檗茎皮64min。用HPLC分别测定了黄檗根皮、茎皮和叶中小檗碱、掌叶防己碱、药根碱的含量,结果表明:小檗碱和药根碱在根皮中的含量最高,茎皮次之,叶最低,而掌叶防己碱在茎皮中的含量最高,根皮次之,叶最低。

关键词: 生物碱, 黄檗, 超声波提取, 高效液相色谱分析

Abstract: A simple, rapid and sensitive method for the determination of alkaloids content in Phellodendron amurense R. was established. Alkaloids was ultrasonically extracted with 63% alcohol at 41℃ for 64 min, and determined by high performance liquid chromatography. The alkaloids were separated on a KYA HIQ sil C18 column 250 mm×4.6 mm, 5 μm, using acetonitrile-aqueous solution 3.4 g KH₂PO₄ and 1.7 g SDS in 1 000 mL H₂O 1:1 as the mobile phase at flow rate 1.0 mL/min and detected at 345 nm. Contents of berberine, palmatine and jatrorrhizine in the root bark, stem bark and leaves of P. amurense R. were determined. The results showed that alkaloids contents in the root bark, stem bark and leaves were different. Contents of berberine and jatrorrhizine were higher in the root bark than in the stem bark, and they were the lowest in the leaves; while palmatine content was the highest in the stem bark, then in the root bark, and the lowest in the leaves too.

Key words: alkaloids, Phellodendron amurense Rupr., ultrasonic extraction, HPLC

中图分类号:

秦彦杰;张玉红;王洋;阎秀峰. 黄檗中生物碱含量的高效液相色谱分析[J]. 林产化学与工业, 2004, 24(S1): 115-118.

QIN Yan-jie;ZHANG Yu-hong;WANG Yang;YAN Xiu-feng. DETERMINATION OF ALKALOIDS CONTENT IN PHELLODENDRON AM URENSE RUPR. BY HPLC[J]. , 2004, 24(S1): 115-118.

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