表棓儿茶素棓酸酯体外氧化及其低聚产物形成的研究

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (5): 62-66.

表棓儿茶素棓酸酯体外氧化及其低聚产物形成的研究

王静, 戚向阳, 朱学良, 李蕙蕙, 刘传菊

华中农业大学食品科技学院, 湖北武汉 430070

收稿日期:2006-11-13 修回日期:1900-01-01 出版日期:2007-10-30 发布日期:2007-10-30
通讯作者: 戚向阳,博士,硕士生导师,主要从事天然产物及保健功能研究;E-mail:qxiangyang@mail.hzau.edu.cn

Effects of Reaction Condition on the Oxidation in vitro of Epigallocatechin-3-gallate and the Formation of Its Oligomers

WANG Jing, QI Xiang-yang, ZHU Xue-liang, LI Hui-hui, LIU Chuan-ju

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2006-11-13 Revised:1900-01-01 Online:2007-10-30 Published:2007-10-30

摘要/Abstract

摘要： 为控制儿茶素类低聚氧化产物形成条件,以表棓儿茶素棓酸酯(EGCG)为代表进行体外化学氧化,通过对产物的HPLC-MS分析确定研究对象,即EGCG氧化低聚物(EOP),其主要有3种低聚产物:产物1 m/z 911,产物2 m/z 929,产物3 m/z 911.产物1和产物3为EGCG脱氢二聚体;产物2为EGCG醌式二聚体.反应条件的研究表明,随温度升高EGCG氧化程度加剧,EOP生成量也随之增加,但温度达40℃时,EOP的生成量在氧化10 min后下降;体系pH值增大可加剧EGCG氧化,但pH值9.0时,EOP的生成量在15 min后有所下降;随氧化剂所占质量比的增大,EGCG氧化程度加大,但EOP的生成量并不与之成正相关. EOP形成的最佳条件为:反应温度25℃,pH值7.5,氧化剂与底物的质量比1.5:1,氧化时间1 5min.

关键词: 表棓儿茶素棓酸酯, 氧化低聚物, 化学氧化

Abstract: In order to control the formation condition of oxidation oligomers of flavanols, epigallocatechin-3-gallate (EGCG) was oxidized in vitro and EGCG oligomeric products (EOP) were determined by HPLC-MS analysis. The influences of temperature, pH value, mass ratio of oxidant to EGCG on the oxidation of EGCG and the formation of EOP have been discussed. HPLC-MS analysis showed that there were mainly 3 oligomers, namely product 1 m/z 911, product 2 m/z 929, product 3 m/z 911. Products 1 and 3 were dehydrodimers of EGCG, product 2 was quinone dimer of EGCG. The study of reaction condition showed that oxidation of EGCG was enhanced with increased temperature. However when temperature reached 40℃, the formation of EOP decreased after 10 min; EGCG oxidation reaction could be accelerated with increase of pH value. However, at pH value 9.0, the formation of EOP decreased after 15 min; EGCG oxidation yield was increased with increase of m(oxidant):m(EGCG). However, the formation of EOP did not have a correlated relationship. The optimum conditions of EOP formation were:temperature 25℃, pH value 7.5, m(oxidant):m(EGCG)1.5:1,oxidation time 15 min.

Key words: epigallocatechin-3-gallate, oligomeric products, chemical oxidation

中图分类号:

TQ91

王静;戚向阳;朱学良;李蕙蕙;刘传菊. 表棓儿茶素棓酸酯体外氧化及其低聚产物形成的研究[J]. 林产化学与工业, 2007, 27(5): 62-66.

WANG Jing;QI Xiang-yang;ZHU Xue-liang;LI Hui-hui;LIU Chuan-ju. Effects of Reaction Condition on the Oxidation in vitro of Epigallocatechin-3-gallate and the Formation of Its Oligomers[J]. , 2007, 27(5): 62-66.