虎杖不同组织部位及其愈伤组织中白藜芦醇含量的测定

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (S1): 109-112.

虎杖不同组织部位及其愈伤组织中白藜芦醇含量的测定

雷勇¹, 曹庸¹, 陈雪香², 李伟³

1. 华南农业大学食品学院, 广东广州 510642;2. 湖南农业大学生命科学技术学院, 湖南长沙 410128;3. 吉首大学生物资源与环境科学学院, 湖南吉首 416000

收稿日期:2007-06-20 修回日期:1900-01-01 出版日期:2007-10-20 发布日期:2007-10-20
通讯作者: 曹庸,教授,博士生导师,主要从事天然生物活性物的研究;E-mail:caoyong2181@scau.edu.cn。

Determination of Resveratrol Content in Different Parts and Calluses of Polygonum cuspidtums Sieb. et Zucc. by HPLC

LEI Yong¹, CAO Yong¹, CHEN Xue-xiang², LI Wei³

1. College of Food Science, South China Agriculture University, Guangzhou 510642, China;2. College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China;3. College of Biology and Environmental Science, Jishou University, Jishou 416000, China

Received:2007-06-20 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

摘要/Abstract

摘要： 通过超声波提取虎杖根状茎中不同组织部位及其不同愈伤组织中的白藜芦醇,并采用HPLC方法检测分析白藜芦醇含量.结果表明:在虎杖根状茎不同组织部位中,白藜芦醇含量(质量分数计)依次为:根状茎芽>髓>木质部>韧皮部>周皮,其中根状茎芽中白藜芦醇质量分数高达0.4251%,髓中白藜芦醇质量分数为0.2664%.在同一批根状茎芽诱导的不同颜色愈伤组织体系中,棕褐色愈伤组织中白藜芦醇质量分数最高为0.0550%,淡黄绿色愈伤组织中仅为0.0005%.光照培养条件下的愈伤组织中白藜芦醇质量分数为0.0104%,黑暗培养条件下为0.0075%.不同外植体诱导的愈伤组织中白藜芦醇质量分数依次为:根状茎芽>茎>叶,以根状茎芽诱导的愈伤组织中白藜芦醇的质量分数最高,为0.0550%.不同组织部位诱导的愈伤组织中白藜芦醇质量分数为:鲜茎(根状茎中韧皮部)>根状茎中木质部>根状茎中髓,鲜茎中达到0.0090%.

关键词: 虎杖, 根状茎, 愈伤组织, 白藜芦醇

Abstract: Resvertarol was extracted by ultrasonic extraction from different tissue parts and calluses of Plygorum cuspidatum Sieb.et Zucc. and analyzed by HPLC. The results showed that the contents of resveratrol in natural resource of P. cuspidatum were in the sequence of: rhizome bud>marrow>xylem>phloem>perithelium. The highest content of resveratrol in rhizome bud is 0.4251%, the content in marrow is 0.2664%.In the same batch of calluses in different colors, the highest content of resvetatrol is 0.0550%, which is the brown one, the lowest contents of resveratrol is only 0.0005%, which is the yellow green one. Under different cultural conditions, the callus cultured under the light has more resvetatrol (mass fraction 0.0104%) than the callus cultured in darkness(0.0075%). In the different explants sources of callus tissue, the content of resveratrol is in the sequence of: rhizome bud>rhizome>leaves, in which rhizome bud with the callus induced has resveratrol content of 0.0550%. Resveratrol mass fraction of callus in different induced parts is: fresh rhizome (phloem in rhizome)>xylem in rhizome>marrow in rhizome, about 0.0090% in fresh rhizome.

Key words: Polygonum cuspidatum Sieb.et Zucc., rhizome, calluses, resveratrol

中图分类号:

TQ91

雷勇;曹庸;陈雪香;李伟. 虎杖不同组织部位及其愈伤组织中白藜芦醇含量的测定[J]. 林产化学与工业, 2007, 27(S1): 109-112.

LEI Yong;CAO Yong;CHEN Xue-xiang;LI Wei. Determination of Resveratrol Content in Different Parts and Calluses of Polygonum cuspidtums Sieb. et Zucc. by HPLC[J]. , 2007, 27(S1): 109-112.

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