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林产化学与工业

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (S1): 85-88.

• 研究报告 • 上一篇    下一篇

高效毛细管电泳法检测印楝素A的初步研究

王有琼, 马李一, 张重权, 段琼芬   

  1. 中国林业科学研究院 资源昆虫研究所, 云南 昆明 650224
  • 收稿日期:2007-06-20 修回日期:1900-01-01 出版日期:2007-10-20 发布日期:2007-10-20
  • 通讯作者: 段琼芬,副研究员,博士,主要从事天然产物的开发利用研究;E-mail:qiongfend@126.com。

Preliminary Study on Determination of Azadirachtin A by High-performance Capillary Electrophoresis

WANG You-qiong, MA Li-yi, ZHANG Zhong-quan, DUAN Qiong-fen   

  1. Research Institute of Resourse Insects, Chinese Academy of Forestry, Kunming 650224, China
  • Received:2007-06-20 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

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摘要： 从理论角度分析了高效毛细管电泳技术检测印楝素A的可能性,并以试验进行证明.在十二烷基硫酸钠(SDS)浓度为25 mmol/L、0.02 mol/L pH值6.86混合磷酸盐缓冲溶液,甲醇-缓冲溶液(体积比7:3)作为运行缓冲液,分离电压20 kV下得到稳定的电流曲线,二极管阵列检测器(PDA)检测波长214 nm,在迁移时间2.6 min处检测到印楝素A的电泳特征峰,电泳谱图面积积分结果和高效液相色谱分析结果无显著差异.

关键词: 高效毛细管电泳, 印楝素A, 检测, 缓冲溶液

Abstract: The possibility of using high-performance capillary electrophoresis (HPCE) to determine azadirachtin A was analysed theoretically, and proved experimentally.The stable current curve had been obtained under the testing condition as follows: SDS concentration 25 mmol/L, phosphate buffer solution concentration 0.02 mol/L, pH value 6.86, volume ratio of methanol-buffer solution 7:3, separation voltage 20 kV. The characteristic electrophoretogram of azadirachtin A was very well examinated by PDA at wavelengh 214 nm and migration time 2.6 min. The result of area integration has no notable difference with that of HPLC.

Key words: high performance capillary electrophoresis, azadirachtin A, determination, buffer solution

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