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林产化学与工业

林产化学与工业 ›› 2008, Vol. 28 ›› Issue (3): 101-103.

• 论文 • 上一篇    下一篇

兴安白芷种子欧前胡素含量的HPLC分析及不同产地的含量测定

姜雪, 王萍, 王洋, 阎秀峰   

  1. 东北林业大学, 生命科学学院, 黑龙江, 哈尔滨, 150040
  • 收稿日期:2007-09-18 修回日期:1900-01-01 出版日期:2008-06-30 发布日期:2008-06-30
  • 通讯作者: 阎秀峰(1965-),男,黑龙江肇东人,教授,博士生导师,主要从事植物生理生态学研究。

Content Determination of Imperatorin in Angelica dahurica(Fisch.)Bench.et Hook.Seeds from Different Habitats by HPLC

JIANG Xue, WANG Ping, WANG Yang, YAN Xiu-Feng   

  1. College of Life Sciences, Northeast Forestry University, Harbin 150040, China
  • Received:2007-09-18 Revised:1900-01-01 Online:2008-06-30 Published:2008-06-30

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摘要： 用高效液相色谱法测定兴安白芷种子中欧前胡素含量.利用均匀设计试验筛选了分析样品的制备方法,确定的最佳提取条件为:以78%乙醇为溶剂在20℃下超声波辅助提取22min,欧前胡素得率0.698%.测定了不同产地兴安白芷种子中欧前胡素的含量,绥阳地区兴安白芷种子中的欧前胡素质量分数最高,达到1.722%;其次为虎林和海林所产,分别为1.519%和1.01%,大兴安岭地区质量分数最低,为0.698%.

关键词: 兴安白芷, 欧前胡素, 高效液相色谱法, 均匀设计

Abstract: A method for content determination of imperatorin in Angelica dahurica(Fisch.) Bench.et Hook.by HPLC was established. The HPLC separation was performed on a hypersil ODS2 column(250mm×4.6mm, 5μm), at flow rate 1 mL/min, with mobile phase of methanol-water (65:35, volume ratio) and detected at wavelength 254nm. Using uniform design, the optimal conditions for imperatorin extraction were explored to be ultrasonically extracted by 78% ethanol at 20℃ for 22min. Imperatorin contents in A.dahurica seeds from different areas were determined. The highest imperatorin content was 1.722%, from Suiyang, then from Hulin and Hailin which were1.519%and 1.031%respectively, and the lowest was 0.698%, from Daxing'anling.

Key words: Angelica dahurica(Fisch.)Bench.et Hook., imperatorin, HPLC, uniform design

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