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林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (1): 117-120.doi: 10.3969/j.issn.0253-2417.2013.01.022

• 1研究报告 • 上一篇    下一篇

HPLC法同时测定凤尾草中5种黄酮类成分的含量

刘建群, 任晓静, 舒积成, 张锐, 张维, 潘景行   

  1. 江西中医学院 现代中药制剂教育部重点实验室, 江西 南昌 330004
  • 收稿日期:2012-01-04 修回日期:1900-01-01 出版日期:2013-02-28 发布日期:2013-02-28

Simultaneous Determination of Five Flavonoids in Pteris multifida Poir. by HPLC

LIU Jian-qun, REN Xiao-jing, SHU Ji-cheng, ZHANG Rui, ZHANG Wei, PAN Jing-hang   

  1. Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
  • Received:2012-01-04 Revised:1900-01-01 Online:2013-02-28 Published:2013-02-28

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摘要： 建立高效液相色谱法,同时测定10个不同产地凤尾草中5种黄酮类成分:新西兰牡荆苷、忍冬苷、野漆树苷、木犀草素和芹菜素的含量。使用Diamonsil C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱(0～10 min, 15:85; 20～30 min, 25:75; 35～40 min, 50:50),流速1.0 mL/min,检测波长 350 nm,柱温25 ℃。结果新西兰牡荆苷、忍冬苷、野漆树苷、木犀草素和芹菜素5个黄酮类化合物在测定的范围内峰面积与浓度之间均表现出良好的线性(r≥0.999,n=5),加样回收率分别为97.18%～105.20%(RSD为1.72%～3.20%)、95.02%～104.92%(RSD为1.96%～2.93%)、98.45%～103.70%(RSD为1.66%～2.70%)、96.41%～104.73%(RSD为1.03%～3.46%)、95.82%～99.34%(RSD为1.85%～3.95%)。结果表明凤尾草中5种黄酮含量的地区差异性不大,各地区凤尾草中5种黄酮类化合物总含量在0.5～2.2 mg/g范围内。

关键词: 凤尾草, 黄酮, 高效液相色谱法

Abstract: An HPLC method was established to the determination of five flavonoids (vicenin-2, lonicerin, rhoifolin, luteolin and apigenin) in Pteris multifida. Separation was carried out on a Diamonsil C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile-water (containing 0.1% formic acid) with gradient elution (0-10 min, 15:85; 20-30 min, 25:75; 35-40 min, 50:50). The flow rate was 1.0 mL/min at 25 ℃, and the detection wavelength was set at 350 nm. The average recoveries for vicenin-2, lonicerin, rhoifolin, luteolin and apigenin were 97.18%-105.20% (RSD1.72%-3.20%), 95.02%-104.92% (RSD 1.96%-2.93%), 98.45%-103.70% (RSD 1.66%-2.70%), 96.41%-104.73% (RSD 1.03%-3.46%), 95.82%-99.34% (RSD 1.85%-3.95%), respectively. These compounds showed good linearity(r≥0.999, n=5) in the detected concentration ranges. There were no significant differences of mass factions of the five flavonoids from different regions. The total mass factions of the five flavonoids were in the range of 0.5-2.2 mg/g.

Key words: Pteris multifida Poir., flavonoids, HPLC

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