全细胞生物催化制备生物柴油研究——固定化细胞的制备及其催化性能

林产化学与工业 ›› 2009, Vol. 29 ›› Issue (1): 18-22.

全细胞生物催化制备生物柴油研究——固定化细胞的制备及其催化性能

李治林¹, 李迅¹, 王飞¹, 蒋剑春²

1. 南京林业大学, 化学工程学院, 江苏南京, 210037;2. 中国林业科学研究院林产化学工业研究所;国家林业局, 林产化学工程重点开放性实验室, 江苏南京, 210042

收稿日期:2008-10-26 修回日期:1900-01-01 出版日期:2009-02-28 发布日期:2009-02-28
通讯作者: 王飞,教授,博士生导师,主要从事生物质能源与化学品、天然产物化学研究;E-mail:hgwf@njfu.edu.cn。

Study on Preparation of Biodiesel by Whole-cell Biocatalysis——Preparation and Catalytic Capability of Immobilized Cells

LI Zhi-lin¹, LI Xun¹, WANG Fei¹, JIANG Jian-chun²

1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;2. Institute of Chemistry and Industry of Forest Products, CAF;Key and Open Lab.on Forest Chemical Engineering, SFA, Nanjing 210042, China

Received:2008-10-26 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28

摘要/Abstract

摘要： 以硅藻土、聚氨酯树脂、氧化铝和海藻酸钠4种载体固定化米根霉细胞,探讨它们催化大豆油甲酯化反应生产生物柴油的能力。结果表明聚氨酯树脂为适宜的固定载体,在80mL液体发酵培养基中,加入聚氨酯树脂0.6g时所制备得到的固定化细胞性能最佳,此时固定上细胞干质量为0.5560g,培养液酶活为17.4U/mL。将此固定化细胞用于催化大豆油甲酯化反应,在m(甲醇):m(大豆油)为5:1甲醇分批加入(每12h加入1批)的情况下,甲酯得率可达94%。

关键词: 米根霉, 固定化细胞, 全细胞生物催化剂, 甲酯化反应

Abstract: Rhizopus oryzae cells were immobilized respectively with 4 different supports, including diatomite, polyurethane foam resin (PUF), alumina and sodium alginate, and the immobilized cells were used to catalyze methylation of soybean oil with methanol. The results showed that PUF was the most suitable immobilizing support for R. oryzae cells. When 0.6gPUF was added into 80mLculture medium, the obtained immobilized cells biocatalyst had optimal performance, in which immobilized dry weight of cells and lipase activity of the culture fluid were 0.5560g and 17.4U/mL, respectively. The yield of methyl esters (ME) from the methylation of soybean with methanol catalyzed by PUF immobilized cells could reach 94%when m(methanol):m(soybean oil) was 5:1 and methanol was added in 5 batches (12h for each batch). The whole-cell biocatalyst of PUF immobilized cells can be simply prepared and separated, and it is environment-friendly and reusable.

Key words: Rhizopus oryzae, immobilized cells, whole-cell biocatalyst, methylation

中图分类号:

TQ351
TQ517

李治林;李迅;王飞;蒋剑春. 全细胞生物催化制备生物柴油研究——固定化细胞的制备及其催化性能[J]. 林产化学与工业, 2009, 29(1): 18-22.

LI Zhi-lin;LI Xun;WANG Fei;JIANG Jian-chun. Study on Preparation of Biodiesel by Whole-cell Biocatalysis——Preparation and Catalytic Capability of Immobilized Cells[J]. , 2009, 29(1): 18-22.

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