关键词: 意大利白杨, 内生真菌, 纤维素, 茎点霉属, 生物柴油

Abstract: An endophytic fungus was screened from the phloem of Populus euramevicana, which can utilize cellulose to produce lipid. It was identified as Phoma sp. Using smashed leaves of P.euramevicana as the sole carbon source, the rules of the fungus to produce microbial oil in deep-liquid fermentation was studied. On the 8th day, thehighest lipid content and filter-paper activity were 0.78 g/L and 5.67 mg/(L·min) (using glucose as substrate) respectively. Various factors were investigated and orthogonal test was used to study their effects on lipid production. The results showed that the optimum condition for producing microbial oil included:corn stalks 50 g/L, NH₄NO₃ 3 g/L, 25℃, 150 r/min. Lipid productivity thus reached 1.12 g/L. The oils produced under this condition were studied by GC, the main components were palmitic acid, stearic acid, oleic acid and linoleic acid, which could be used as raw materials of biodiesel.

Key words: Populus euramevicana, endophytic fungi, cellulose, Phoma sp., biodiesel