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林产化学与工业

林产化学与工业 ›› 2010, Vol. 30 ›› Issue (3): 61-65.

• 研究报告 • 上一篇    下一篇

一种包含双纤维素结合域的新型生物纸张增强剂

郑斐, 石翛然, 吴咪佳, 汪晶, 丁少军   

  1. 南京林业大学 化学工程学院, 江苏 南京 210037
  • 收稿日期:2009-08-10 修回日期:1900-01-01 出版日期:2010-06-30 发布日期:2010-06-30
  • 通讯作者: 丁少军,教授,博士生导师,主要从事工业微生物学和纤维素及相关酶类在生物技术中的应用研究; E-mail:dshaojun@hotmail.com。

A Novel Paper-strengthening Agent Containing Double Cellulose-binding Domains

ZHENG Fei, SHI Xiao-ran, WU Mi-jia, WANG Jing, DING Shao-jun   

  1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Received:2009-08-10 Revised:1900-01-01 Online:2010-06-30 Published:2010-06-30

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摘要： 将两个来源于草菇乙酰木聚糖酯酶的家族I的纤维素结合域(CBD)融合到一起形成重组蛋白,并将该功能性纤维素吸附重组蛋白在大肠杆菌中进行表达,利用该重组蛋白处理Whatman滤纸后检测滤纸的机械性能。检测发现经纯化的重组蛋白处理后的滤纸其机械性能都有所提高, 其中抗张强度提高了10%,耐破度提高了8.7%,耐折度提高了30%。该研究表明CBD在纤维及造纸工业等方面具有巨大的潜在应用价值。

关键词: 乙酰木聚糖酯酶, 纤维素结合域, 机械性能, 纸张增强剂

Abstract: Two family-I cellulose-binding domains(CBD) of acetyl xylan esterase from Volvariella volvacea were fused to form a recombinant protein. The recombinant bifunctional cellulose-binding protein was expressed in E.coli. Then the mechanical properties of Whatman filter paper treated with the recombinant protein were tested. The purified protein improved the treated paper's tensile strength, bursting strength and folding endurance by 10%, 8.7% and 30%, respectively. This research demonstrated that the cellulose-binding domains have an immense potential for the application in the field of fiber and paper industries.

Key words: acetyl xylan esterase, cellulose-binding domain, mechanical properties, paper-strengthening agent

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