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林产化学与工业

林产化学与工业 ›› 2011, Vol. 31 ›› Issue (1): 13-16.

• 研究报告 • 上一篇    下一篇

油茶饼粕中黄酮苷类化合物的分离与结构鉴定

陈虹霞, 王成章, 叶建中, 周昊   

  1. 中国林业科学研究院 林产化学工业研究所;生物质化学利用国家工程实验室; 国家林业局 林产化学工程重点开放性实验室, 江苏 南京 210042
  • 收稿日期:2010-07-20 修回日期:1900-01-01 出版日期:2011-02-28 发布日期:2011-02-28
  • 通讯作者: 王成章,湖北汉川人,研究员,主要从事天然产物研究与利用;E-mail:wangczlhs@sina.com。

Isolation and Identification of Flavonoid Glycosides from Gamellia oleifera Abel. Cake

CHEN Hong-xia, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao   

  1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.on Forest Chemical Engineering, SFA, Nanjing 210042, China
  • Received:2010-07-20 Revised:1900-01-01 Online:2011-02-28 Published:2011-02-28

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摘要： 以茶籽饼粕为原料,采用体积分数65%乙醇-水溶液提取,并通过中低压色谱快速分离得到高纯度的黄酮苷混合物。进一步采用高效液相色谱制备,柱子XB-C18(Φ10mm×250mm, 5μm),流动相甲醇-水(体积比2:3),紫外270nm,流速3mL/min,分离得到黄酮苷化合物Ⅰ和Ⅱ。经IR、MS和NMR鉴定,两种化合物分别为山奈酚3-O-[2-O-β-D-半乳糖-6-O-α-L-鼠李糖]-β-D-葡萄糖苷(Ⅰ)和山奈酚3-O-[2-O-β-D-木糖-6-O-α-L-鼠李糖]-β-D-葡萄糖苷(Ⅱ)。

关键词: 油茶饼粕, 黄酮苷, 高效液相色谱, 分离

Abstract: The dreg-cakes of oil camellia(Camellia deifera Abel.)were extracted with 65% ethanol. The extract was separated by middle-pressure chromatography. The flavoniod glycosides mixture in high purity was obtained two compounds were isolated by HPLC, using XB-C18 column (Φ10mm×250mm,5μm) with a mobile phase which consists of a mixture of methanol and water (volume ratio 2:3). It had a flow rate of 3.0mL/min. UV detection was made at 230nm. According of IR, MS and NMR, the two compounds were identified to be kaempferol 3-O-[2-O-β-D--galactopyranosy-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside(Ⅰ)and kaempferol 3-O-[2-O-β-D-xylocopyranosy-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside(Ⅱ), respectively.

Key words: Camellia oleifera Abel. cake, flavonoid glycosides, HPLC, isolation

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