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林产化学与工业

林产化学与工业 ›› 2011, Vol. 31 ›› Issue (6): 7-10.

• 研究报告 • 上一篇    下一篇

两步实验设计优化栅藻产油条件的工艺研究

杨静, 蒋剑春, 张宁, 卫民   

  1. 中国林业科学研究院 林产化学工业研究所;生物质化学利用国家工程实验室; 国家林业局 林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏 南京 210042
  • 收稿日期:2011-03-02 修回日期:1900-01-01 出版日期:2011-12-30 发布日期:2011-12-30
  • 通讯作者: 蒋剑春,研究员,博士,博士生导师,研究领域为林产化学加工和生物质能源开发技术;E-mail:bio-energy@163.com。

A Two-step Statistical Optimization of Cultivation Conditions for Producing Lipid by Scenedesmus obliquus

YANG Jing, JIANG Jian-chun, ZHANG Ning, WEI Min   

  1. Institute of Chemical Industry of Forest Products,CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.on Forest Chemical Engineering,SFA;Key Lab.of Biomass Energy and Material,Jiangsu Province, Nanjing 210042, China
  • Received:2011-03-02 Revised:1900-01-01 Online:2011-12-30 Published:2011-12-30

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摘要： 提出了一种简单、快速优化栅藻(Scenedesmus obliquus)产油条件的两步实验设计方法。首先,利用部分析因设计方法从6个因素(光照强度,温度,pH值,接种量,葡萄糖浓度,氮源浓度)中筛选出显著因素。再利用正交试验进一步确定出最优条件。结果表明:氮源浓度和温度是影响栅藻产油的显著因素。适合培养栅藻产油的最优条件为:氮源 6.0 mmol/L,温度 25 ℃ 和光照 80 μmol/(m2· s), 最高油脂产率达到 75.46 mg/(L ·d)。

关键词: 栅藻, 部分析因设计, 正交试验, 油脂产率

Abstract: A fast and easy two-step statistical optimization of cultivation condition for producing lipid by Scenedesmus obliquus was described. At first, a fractional factorial design (FFD) was used to screen signi-ficant factors from six factors (illumination, temperature, pH value, inoculation, glucose concentration and nitrogen source concentration). Then an orthogonal experiment was executed to further determine optimal conditions. According to the results, nitrogen source concentration and temperature were selected as significant factors. The optimal conditions occurred at nitrogen source concentration 6.0 mmol/L, temperature 25 ℃ and illumination 80 μmol/(m2· s), with the highest lipid productivity of 75.46 mg/(L·d).

Key words: Scenedesmus obliquus, fractional factorial design, orthogonal design, lipid productivity

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