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林产化学与工业

林产化学与工业 ›› 2011, Vol. 31 ›› Issue (5): 11-17.

• 研究报告 • 上一篇    下一篇

杨木屑低浓度硫酸中温预处理提取木糖及其发酵

徐勇1,2,3, 焦杰1, 徐斌伟1, 勇强1,2, 余世袁1,2   

  1. 1. 南京林业大学 化学工程学院, 江苏 南京 210037;2. 林木遗传与生物技术教育部重点实验室, 江苏 南京 210037;3. 江苏省生物质绿色燃料与化学品重点实验室, 江苏 南京 210037
  • 收稿日期:2011-06-09 修回日期:1900-01-01 出版日期:2011-10-30 发布日期:2011-10-30

Extraction and Fermentation of Xylose from Poplar Sawdust by Hot Sulfuric Acid Pretreatment at Low Concentration

XU Yong1,2,3, JIAO Jie1, XU Bin-wei1, YONG Qiang1,2, YU Shi-yuan1,2   

  1. 1. College of Chemical Engineering,Nanjing Forestry University, Nanjing 210037, China;2. Key Laboratory of Forest Genetics & Biotechnology,MED, Nanjing 210037, China;3. Jiangsu Key Lab of Biomass-based Green Fuel & Chemicals, Nanjing 210037, China
  • Received:2011-06-09 Revised:1900-01-01 Online:2011-10-30 Published:2011-10-30

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摘要： 低酸中温预处理可选择性地水解溶出杨木屑中的木糖。正交试验结果显示,影响木糖提取得率的强度顺序为反应温度、固液比、反应时间和硫酸质量分数,影响抑制物产量的主要因素为反应温度和固液比,硫酸质量分数和反应时间的影响较弱。在预处理过程中生成的主要抑制物为乙酸,其次为甲酸和糠醛。基于技术经济分析,可采用 100 ℃、 2.0 % 硫酸、固液比1∶15(g∶mL)预处理杨木屑 10 h,木糖和葡萄糖提取得率分别达 74.1 % 和 3.0 %。通过细胞回用和驯化可以提高Candida tropicalis NL02发酵杨木屑提取液的生产性能,在含有乙酸 3.53 g/L、甲酸 2.57 g/L、木糖 53.40 g/L 和葡萄糖 7.09 g/L 的提取液中发酵 20 h,木糖利用率达 85.4 %,乙酸和甲酸消耗率分别为 52.9 % 和 100 %,可生成木糖醇约 35.12 g/L 和乙醇 7.67 g/L,木糖醇和乙醇产率分别达到 77.0 % 和 31.4 %。

关键词: 木糖, 提取, 发酵, 杨木屑, 低酸中温预处理, 热带假丝酵母

Abstract: Xylose was selectively hydrolyzed and extracted from poplar sawdust by hot sulfuric acid pretreatment at low concentration (HSAP). The orthogonal test showed the impact strength of main processing parameters on xylose extraction was descending as follows: temperature, the solid-to-liquid ratio (SLR), sulfuric acid concentration (SAC) and reaction time (RT). The total inhibitors production was affected mainly by temperature and SLR, while, SAC and RT had little effects on it. Acetic acid came from poplar sawdust as the first major inhibitor during HSCP and the secondary were formic acid and furfural. The optimum HSCP condition was selected according to techno-economical analysis, in which about 74.1 % xylose and 3.0 % glucose were extracted from poplar sawdust after 10 h at 100 ℃, 2.0 % SA and SLR of 1∶15. The fermentability of Candida tropicalis NL02 was improved by yeast recycling and domestication in the extracted solution from poplar sawdust by HSCP. In the extracted solution containing 3.53 g/L acetic acid, 2.57 g/L formic acid 53.40 g/L xylose and 7.09 g/L glucose, the yeast could use up 85.4 % xylose, 52.9 % acetic acid and 100 % formic acid, and produced 35.12 g/L xylitol and 7.67 g/L ethanol at the yield of 77.0 % and 31.4 %, respectively.

Key words: xylose, extraction, fermentation, poplar sawdust, hot sulfuric acid pretreatment at low concentration, Candida tropicalis

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