糠醛渣/木薯渣混合底物同步糖化发酵转化乙醇研究

doi:10.3969/j.issn.0253-2417.2016.02.018

林产化学与工业 ›› 2016, Vol. 36 ›› Issue (2): 121-128.doi: 10.3969/j.issn.0253-2417.2016.02.018

糠醛渣/木薯渣混合底物同步糖化发酵转化乙醇研究

吉骊¹, 赵鹏翔^1,2, 游艳芝¹, 卜令习², 蒋建新¹

1. 北京林业大学材料科学与技术学院, 林业生物质材料与能源教育部工程中心, 北京 100083;
2. 国网节能服务有限公司北京生物质能源技术中心, 北京 100053

收稿日期:2015-07-07 出版日期:2016-04-25 发布日期:2016-04-26
通讯作者: 蒋建新,教授,博士生导师,研究领域为林产化工及生物质能源;E-mail:jiangjx@bjfu.edu.cn。 E-mail:jiangjx@bjfu.edu.cn
作者简介:吉骊(1991—),女,甘肃兰州人,硕士生,主要从事生物质能源及化学品相关研究工作
基金资助:
国家自然科学基金资助项目(31070510);国际科技合作专项(2014DFG32550);国网科技项目(SGECS56-2014)

Ethanol Production from Mixture of Furfural Residues and Cassava Residues by Simultaneous Saccharification and Fermentataion

JI Li¹, ZHAO Peng-xiang^1,2, YOU Yan-zhi¹, BU Ling-xi², JIANG Jian-xin¹

1. College of Material Science and Technology, MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 10083, China;
2. Beijing Biomass Energy Technology Center, State Grid Energy Conservation Service Ltd., Beijing 100053, China

Received:2015-07-07 Online:2016-04-25 Published:2016-04-26

摘要/Abstract

摘要： 研究了糠醛渣(FR)经不同强度绿液-过氧化氢预处理脱木质素后,与木薯渣(CR)混合进行同步糖化发酵生产乙醇,通过改变原料底物浓度、纤维素酶用量和添加无患子表面活性剂来优化混合底物同步糖化发酵条件,并分析了发酵过程中乙醇和副产物的浓度变化。结果表明,在糠醛渣预处理条件为:底物质量浓度5 g/L、温度80 ℃、H₂O₂用量为0.6 g/g、绿液用量为2 mL/g(以糠醛渣计)预处理时间3 h,在此条件下糠醛渣木质素脱除率可达56.5%。同步糖化发酵产乙醇条件为无患子皂素表面活性剂添加量0.5 g/L,纤维素酶用量12 FPU/g,纤维二糖酶用量15 IU/g,预处理后的糠醛渣与木薯渣混合作底物(质量比为2:1),底物质量浓度200 g/L时,发酵120 h最终乙醇质量浓度可达56.6 g/L,乙醇得率为86.3%。同步糖化发酵过程中添加无患子皂素表面活性剂不仅降低了纤维素酶用量,还可延缓副产物乳酸的形成,减小甘油生产波动。

关键词: 糠醛渣, 木薯渣, 混合底物, 无患子皂素, 同步糖化发酵, 乙醇

Abstract: Simultaneous saccharification and fermentation (SSF) of furfural residues (FR) pretreated with green liquor and hydrogen peroxide (GL-H₂O₂) mixed with cassava residues(CR)with the addition of Sapindus mukurossi saponin to product ethanol was investigated in present study. The SSF was optimized by changing the substrate concentration, dosage of cellulase and the addition of saponin, and the corresponding concentrations of ethanol and by-products were analyzed. The results indicated that 56.5% furfural lignin was removed after 5 g/L substrate was treated by 0.6 g/g H₂O₂ and 2 mL/g GL, at 80 ℃ for 3 h. The ethanol concentration after SSF was 56.6 g/L, which was equivalent to the theoretical ethanol yield of 86.3%, as the mixture of pretreated FR and cassava residues with a mass ratio of 2:1 were fermented with the aid of 0.5 g/L Sapindus mukurossi saponin, 12 FPU/g cellulose, and 15 IU/g cellobiose enzyme, for 120 h. Moreover, the amounts of cellulose and generation of by-products were declined when the Sapindus mukurossi saponin was added, and the fluctuation of glycerol production was minimized.

Key words: furfural residues, cassava residues, mixed substrates, Sapindus mukurossi saponin, simultaneous saccharification and fermentation, ethanol

中图分类号:

TQ35

吉骊, 赵鹏翔, 游艳芝, 卜令习, 蒋建新. 糠醛渣/木薯渣混合底物同步糖化发酵转化乙醇研究[J]. 林产化学与工业, 2016, 36(2): 121-128.

JI Li, ZHAO Peng-xiang, YOU Yan-zhi, BU Ling-xi, JIANG Jian-xin. Ethanol Production from Mixture of Furfural Residues and Cassava Residues by Simultaneous Saccharification and Fermentataion[J]. Chemistry and Industry of Forest Products, 2016, 36(2): 121-128.

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糠醛渣/木薯渣混合底物同步糖化发酵转化乙醇研究

Ethanol Production from Mixture of Furfural Residues and Cassava Residues by Simultaneous Saccharification and Fermentataion

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