混菌戊糖己糖发酵产乙醇的研究

doi:10.3969/j.issn.0253-2417.2015.02.017

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (2): 111-116.doi: 10.3969/j.issn.0253-2417.2015.02.017

混菌戊糖己糖发酵产乙醇的研究

李晴^1,2, 宋向阳^1,2, 范晓阳^1,2, 欧阳嘉^1,2, 勇强^1,2

1. 南京林业大学化学工程学院, 江苏南京 210037;
2. 江苏省生物质绿色燃料与化学品重点实验室, 江苏南京 210037

收稿日期:2014-03-04 出版日期:2015-04-25 发布日期:2015-04-24
通讯作者: 宋向阳,教授,博士,主要从事酶工程、发酵工程方面的研究;E-mail:xiangyangsong@hotmail.com。 E-mail:xiangyangsong@hotmail.com
作者简介:李晴(1990-),女,江苏徐州人,硕士生,研究方向为生物化工
基金资助:
国家自然科学基金资助项目(30871992);江苏省科技支撑计划项目(BE2010732);江苏高校优势学科建设工程资助项目(无编号)

Production of Ethanol by Fermentation of Glucose and Xylose Using Mixed Yeasts

LI Qing^1,2, SONG Xiang-yang^1,2, FAN Xiao-yang^1,2, OUYANG Jia^1,2, YONG Qiang^1,2

1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
2. Jiangsu Key Lab of Biomass-based Green Fuel and Chemicals, Nanjing 210037, China

Received:2014-03-04 Online:2015-04-25 Published:2015-04-24

摘要/Abstract

摘要： 比较研究休哈塔假丝酵母单菌发酵、休哈塔假丝酵母和酿酒酵母两步发酵、休哈塔假丝酵母和酿酒酵母同步发酵3种发酵模式下对混合糖(葡萄糖和木糖)的利用情况,结果显示2种酵母同步发酵能够有效解除葡萄糖的抑制,加快木糖利用速率。采用Plackett-Burman 实验、最陡爬坡实验和中心组合设计,对2种酵母混菌利用戊糖己糖发酵生产乙醇的培养基进行优化,得到最优培养基条件:硫酸铵9.09 g/L、磷酸二氢钾8.96 g/L、氯化钙0.34 g/L,在此条件下发酵得到乙醇产量为21.71 g/L,较优化前(19.85 g/L)提高了9.4%;混菌在优化后的培养基下进行发酵,酵母浊度OD由12.12增长到了21.87,木糖利用率由优化前的82%增长到优化后的93%。

关键词: 混合糖, 混菌发酵, 乙醇, 培养基, 优化

Abstract: Mixed sugars(glucose and xylose)consumption patterns by the following three fermentation methods, i.e., single bacterium fermentation by Candida shehatae, successive fermentation by C.shehatae and Saccharomyces cerevisiae, as well as synchronization(fermentation by these two), were investigated. The results showed that the fermentation by synchronization pattern can effectively terminate the inhibition of glucose, and accelerate the xylose utilization at the same time. The fermentation medium of mixed yeast was optimized by Plackett-Burman design, steepest climbing experimental design, and central composite design. The optimal culture conditions were (NH₄)₂SO₄ 9.09 g/L, KH₂PO₄ 8.96 g/L and CaCl₂ 0.34 g/L. Under the above conditions of fermentation, the ethanol yield was 21.71 g/L, which is 9.4 % higher than before. Under the optimized medium, the yeast turbidity (OD) increased from 12.12 to 21.87, and the conversion of xylose also increased from 82 % to 93 %.

Key words: mixed sugar, fermentation of mixed yeasts, ethanol, medium, optimization

中图分类号:

TQ35
TQ920.6

李晴, 宋向阳, 范晓阳, 欧阳嘉, 勇强. 混菌戊糖己糖发酵产乙醇的研究[J]. 林产化学与工业, 2015, 35(2): 111-116.

LI Qing, SONG Xiang-yang, FAN Xiao-yang, OUYANG Jia, YONG Qiang. Production of Ethanol by Fermentation of Glucose and Xylose Using Mixed Yeasts[J]. Chemistry and Industry of Forest Products, 2015, 35(2): 111-116.

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混菌戊糖己糖发酵产乙醇的研究

Production of Ethanol by Fermentation of Glucose and Xylose Using Mixed Yeasts

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