解结晶对纤维素吸附纤维素酶的影响

doi:10.3969/j.issn.0253-2417.2016.01.015

林产化学与工业 ›› 2016, Vol. 36 ›› Issue (1): 105-111.doi: 10.3969/j.issn.0253-2417.2016.01.015

解结晶对纤维素吸附纤维素酶的影响

张景强¹, 李清春¹, 林鹿²

1. 电子科技大学中山学院化学与生物工程学院, 广东中山 528402;
2. 厦门大学能源学院, 福建厦门 361005

收稿日期:2014-12-30 出版日期:2016-02-25 发布日期:2016-03-18
通讯作者: 林鹿,教授,博士生导师,主要从事植物资源化学与生物技术领域研究工作;E-mail:lulin@xmu.edu.cn。 E-mail:lulin@xmu.edu.cn
作者简介:张景强(1974-),男,四川西昌人,副教授,博士,从事植物资源化学与农产品研究
基金资助:
国家自然科学基金-广东自然科学基金联合基金重点项目(U0733001)

Effect of Decrystallization of Cellulose on Adsorbing Cellulase

ZHANG Jing-qiang¹, LI Qing-chun¹, LIN Lu²

1. College of Chemistry & Biology Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China;
2. College of Energy, Xiamen University, Xiamen 361005, China

Received:2014-12-30 Online:2016-02-25 Published:2016-03-18

摘要/Abstract

摘要： 为探讨解结晶处理提高酶水解效率的机理,对比研究了高结晶度的微晶纤维素(MCC)和低结晶度的解结晶微晶纤维素(D-MCC)对纤维素酶的吸附过程。结果发现:酶吸附平衡后,样品的XPS和FT-IR分析表明纤维素酶通过酶蛋白的氨基(-NH₂)与纤维素分子链上的羟基(-OH)之间以氢键(C-OH…NH)力吸附在MCC上;相同条件下得到的MCC和D-MCC吸附率曲线的差异表明,底物结构的改善比提高温度更能提高酶吸附率;比较纤维素酶对MCC和D-MCC在35℃下的吸附动力学可知,通过降低底物的结晶度可促进酶吸附,刚性结构的MCC更满足准一级动力学的假设,D-MCC表面结构相对松散,其吸附过程需进一步分析,以得到更适合的动力学方程。

关键词: 纤维素酶, 微晶纤维素, 解结晶, 吸附

Abstract: The cellulase adsorbed and formed enzyme-substrate(ES) compound is a key step in the process of enzymatic hydrolysis.Through the comparison of cellulase adsorbed on two substrates: microcrystalline cellulose(MCC) with high degree of crystallinity and decrystallization microcrystalline cellulose(D-MCC) with low degree of crystallinity, it was found that, in the XPS wide scan patterns of adsorption equilibrium MCC, a new absorption peak of nitrogen element(399.06 eV) was observed.The fitting method is adopted to treat the strong peak of XPS-O1s, and the binding energy of this new peak was only 530.53 eV.In the infrared absorption range of 3050-3550 cm^-1, the absorption intensity of OH group decreased with extending adsorption time on cellulose.The above results proved that new hydrogen bonds (C-OH…NH) between the protein amino(-NH₂) and cellulose molecule chain(-OH) were generated during the cellulose adsorption process.From the comparison of the adsorption rate curves between MCC and D-MCC, the decrystallization method to improve cellulase adsorption was more effective than enhancing the temperature.From the comparison of adsorption kinetics between MCC and D-MCC at 35℃, it was indicated that a reduction of cellulose crystallinity was positive for adsorption rate.MCC with rigid surface was fit for the first-order kinetic hypothesis.D-MCC with flexible surface was not suitable for the first-order kinetic.A more suitable kinetic hypothesis should be investigated in the future.

Key words: cellulase, microcrystalline cellulose, decrystallization, adsorption

中图分类号:

TQ35

张景强, 李清春, 林鹿. 解结晶对纤维素吸附纤维素酶的影响[J]. 林产化学与工业, 2016, 36(1): 105-111.

ZHANG Jing-qiang, LI Qing-chun, LIN Lu. Effect of Decrystallization of Cellulose on Adsorbing Cellulase[J]. Chemistry and Industry of Forest Products, 2016, 36(1): 105-111.

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解结晶对纤维素吸附纤维素酶的影响

Effect of Decrystallization of Cellulose on Adsorbing Cellulase

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