碱性离子液体TBAH预处理对桉木结构和酶解性能的影响

doi:10.3969/j.issn.0253-2417.2022.01.008

摘要/Abstract

摘要：

为探究碱性离子液体四丁基氢氧化铵(TBAH)在桉木预处理中作用机理，采用响应面分析法设计模型，得到离子液体TBAH预处理桉木的最佳条件为：预处理时间57.19 min，预处理温度71.98℃，TBAH质量分数11.78%，并验证了模型的科学性、准确性和有效性。通过对比未处理、NaOH、四丁基氟化铵(TBAF)和TBAH预处理桉木样品的组成成分，并利用傅里叶红外光谱(FT-IR)、X射线衍射(XRD)和扫描电子显微镜(SEM)来分析预处理的作用机理。研究结果表明：预处理中[TBA]⁺可以有效地降解木质素，降低结晶度；OH^-可以降解半纤维素；在[TBA]⁺和OH^-两者共同作用下桉木样品半纤维素由26.8%降至18.0%，木质素由26.3%降至15.0%；结晶指数由42.39%提高至54.11%；并且TBAH预处理会破坏桉木样品表面完整、致密和均匀的结构；TBAH预处理后的桉木样品经72 h酶解，还原糖产率为365.62 mg/g，纤维素转化率69.24%。

关键词: 碱性离子液体, 预处理, 酶解效率, 响应面分析法

Abstract:

In order to explore the mechanism of the alkaline ionic liquids tetrabutylammonium hydroxide (TBAH) in the pretreatment of eucalyptus, the response surface analysis method was used to design the model, and the optimal conditions[JP]for the pretreatment of eucalyptus with ionic liquid TBAH were pretreatment time 57.19 min, pretreatment[JP]temperature 71.98℃, and TBAH mass fraction 11.78%.The model was proved to be scientific accurate and practical.By comparing the composition[JP]of feedstock, NaOH, tetrabutylammonium fluoride (TBAF) and TBAH pretreated eucalyptus samples, Fourier infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) were carried out for comparing the composition of feedstock with that of NaOH, tetrabutylammonium fluaride (TBAF) and TBAH pretreating eucalyptus samples, which aimed to analyze pretreatment reaction mechanism.The results showed that in the pretreatment, [TBA]⁺ could effectively degrade lignin and reduce the crystallinity; OH^- could degrade hemicellulose.Under the combined action of[TBA]⁺ and OH^-, the hemicellulose of eucalyptus samples was degraded from 26.8% to 18.0%, and the lignin was degraded from 26.3% to 15.0%;the crystallization index increased[JP]from 42.39% to 54.11%;and TBAH pretreatment would destroy the complete, dense and uniform structure of the surface of eucalyptus samples; TBAH pretreated eucalyptus wood sample was enzymatically hydrolyzed for 72 h, the yield of reducing sugar was 365.62 mg/g, and the conversion rate of cellulose was 69.24%.

Key words: alkaline ionic liquid, pretreatment, enzymatic hydrolysis efficiency, response surface analysis

中图分类号:

TQ35

王淑捷, 侯贤锋, 黄颖诗, 陈小凤, 孙瑾, 高振忠. 碱性离子液体TBAH预处理对桉木结构和酶解性能的影响[J]. 林产化学与工业, 2022, 42(1): 57-63.

Shujie WANG, Xianfeng HOU, Yingshi HUANG, Xiaofeng CHEN, Jin SUN, Zhenzhong GAO. Effect of Alkaline Ionic Liquid TBAH Pretreatment on Structure and Enzymatic Properties of Eucalyptus[J]. Chemistry and Industry of Forest Products, 2022, 42(1): 57-63.

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方差来源 variance source	平方和 sum of squares	自由度 d_f	均方值 mean square	F值 F value	P值 P value	显著性 significant
模型model	30647.29	9	3405.254	14.68	0.00023	**
X₁	236.6263	1	236.6263	1.02	0.339075
X₂	839.5086	1	839.5086	3.62	0.089661
X₃	8166.023	1	8166.023	35.17	0.000221	**
X₁X₂	313.1253	1	313.1253	1.35	0.275376
X₁X₃	215.6998	1	215.6998	0.93	0.360284
X₂X₃	0.445598	1	0.445598	0.002	0.966012
X₁²	5121.787	1	5121.787	22.06	0.001125	**
X₂²	16643.41	1	16643.41	71.69	0.000014	**
X₃²	8157.244	1	8157.244	35.14	0.000221	**
残差residual	2089.481	9	232.1645
失拟项lack of fit	1607.604	5	321.5209	2.67	0.181422
纯误差pure error	481.8766	4	120.4691
总和cor total	32736.77	18

样品 sample	纤维素/% cellulose	半纤维素/% hemicellulose	木质素/% lignin	灰分/% ash	还原糖产率/(mg·g^-1) sugar yield
未处理feedstock	42.4	26.8	26.3	0.1	125.73
TBAF预处理TBAF pretreatment	50.5	22.9	15.9	0.1	301.43
NaOH预处理NaOH pretreatment	50.2	16.3	22.3	0.5	356.91
TBAH预处理TBAH pretreatment	52.8	18.0	15.0	0.6	365.62

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