靶向分子印迹吸附材料分离林源活性成分的研究进展

doi:10.3969/j.issn.0253-2417.2022.04.016

摘要/Abstract

摘要：

我国森林资源储量丰富，其中对人类健康有益的各种林源活性成分的开发利用已成为林业产业发展的一个重要方向和途径。随着我国以林源活性成分为重要物质基础的林业生物制剂产业的快速发展，靶向分子印迹吸附材料在林源活性成分的精准分离和高值化加工中具有重要的产业化应用价值。本文介绍了靶向分子印迹技术的分离原理和分类，以及近年来几种新型靶向分子印迹吸附材料的载体类型，主要包括石墨烯、二氧化硅、碳量子点以及生物基载体。重点阐述了靶向分子印迹吸附材料在分离黄酮、多酚、有机酸、生物碱等林源活性成分方面的应用现状，最后对靶向分子印迹材料用于分离林源活性成分的发展进行了总结和展望，分析了靶向分子印迹吸附材料存在的局限性及靶向分子印迹吸附材料在林源活性成分分离应用中的发展方向。

关键词: 靶向分子印迹吸附材料, 林源活性成分, 特异性分离, 载体

Abstract:

China was rich in forest resources, and it was an important direction and approach of the development of forestry industry for exploiting the active components of forest resources which were beneficial to human health. With the rapid development of forestry bio-agent industry based on the active components as important material basis in China, targeted molecular imprinting materials have important industrial application value in the accurate separation and high-value processing of forest active components. This article introduced the principle and classification of targeted molecular imprinting technology, as well as the carrier types of several new-style targeted molecularly imprinted adsorption materials in recent years, mainly include graphene, silica, carbon dots and bio-based carriers. It focused on the application status in the separation of flavonoids, polyphenols, alkaloids, organic acids and other forest source active ingredients by targeted molecularly imprinted adsorption materials. Finally, the development of targeted molecular imprinting materials for the separation of active components from forest sources was summarized and prospected, the limitation of targeted molecular imprinting materials and the development trend of targeted molecular imprinting materials in the separation of active components from forest sources were also analyzed.

Key words: targeted molecular imprinting materials, active components from forest sources, specific separation, carrier

中图分类号:

TQ35
TQ9

吕慕洁, 王立涛, 王建栋, 曹劲松, 靳豪杰, 付玉杰. 靶向分子印迹吸附材料分离林源活性成分的研究进展[J]. 林产化学与工业, 2022, 42(4): 107-118.

Mujie LYU, Litao WANG, Jiandong WANG, Jinsong CAO, Haojie JIN, Yujie FU. Research Progress on Separation of Active Components from Forest Sources by Targeted Molecular Imprinting Materials[J]. Chemistry and Industry of Forest Products, 2022, 42(4): 107-118.

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参考文献 64

1	杨少宗, 刘本同, 钱华, 等. 林源活性成分纳米载体的安全性评价研究[J]. 安徽农业科学, 2018, 46 (32): 102- 105. doi: 10.3969/j.issn.0517-6611.2018.32.030
	YANG S Z , LIU B T , QIAN H , et al. Safety evaluation on the nanocarrier of the active ingredients from forest[J]. Journal of Anhui Agricultural Sciences, 2018, 46 (32): 102- 105. doi: 10.3969/j.issn.0517-6611.2018.32.030
2	祖元刚, 付旷. 林源活性物质分离技术[M]. 北京: 科学出版社, 2015.
	ZU Y G , FU K . Forest Source Active Substance Separation Technology[M]. Beijing: Science Press, 2015.
3	周媛媛, 孟子晖, 董美伶. 分子印迹技术在天然产物有效成分分离纯化中的应用[J]. 色谱, 2009, 27 (3): 359- 363. doi: 10.3321/j.issn:1000-8713.2009.03.022
	ZHOU Y Y , MENG Z H , DONG M L . Application of molecular imprinting technology in separation and purification of active components of natural products[J]. Chinese Journal of Chromatography, 2009, 27 (3): 359- 363. doi: 10.3321/j.issn:1000-8713.2009.03.022
4	曾维星, 程贤, 张笮晦, 等. 生物预处理技术在天然产物提取中的应用研究进展[J]. 生物质化学工程, 2020, 54 (5): 45- 52. doi: 10.3969/j.issn.1673-5854.2020.05.007
	ZENG W X , CHENG X , ZHANG Z H , et al. Application research progress of biological pretreatment technology in extraction of natural products[J]. Biomass Chemical Engineering, 2020, 54 (5): 45- 52. doi: 10.3969/j.issn.1673-5854.2020.05.007
5	HE S N, ZHANG L P, BAI S K, et al. Advances of molecularly imprinted polymers(MIP) and the application in drug delivery[J/OL]. European Polymer Journal, 2021, 143: 110179[2021-04-20]. https://doi.org/10.1016/j.eurpolymj.2020.110179.
6	李春兰. 分子印迹技术在药物分析中的应用[J]. 化工管理, 2020, (22): 41- 42. doi: 10.3969/j.issn.1008-4800.2020.22.019
	LI C L . Application of molecular imprinting technology in drug analysis[J]. Chemical Management, 2020, (22): 41- 42. doi: 10.3969/j.issn.1008-4800.2020.22.019
7	徐硕, 邝咏梅, 吴学军, 等. 分子印迹技术在中药研究中的应用[J]. 西北药学杂志, 2019, 34 (3): 422- 425. doi: 10.3969/j.issn.1004-2407.2019.03.030
	XU S , KUANG Y M , WU X J , et al. Advances on molecular imprinting technology in the application of traditional Chinese medicine[J]. Northwestern Journal of Pharmacy, 2019, 34 (3): 422- 425. doi: 10.3969/j.issn.1004-2407.2019.03.030
8	左振宇, 张光辉, 雷福厚, 等. 分子印迹聚合物在中药活性成分分离中的应用进展[J]. 中草药, 2017, 48 (23): 5019- 5031. doi: 10.7501/j.issn.0253-2670.2017.23.031
	ZUO Z Y , ZHANG G H , LEI F H , et al. Application progress of molecularly imprinted polymers in separation of active ingredients from Chinese materia medica[J]. Chinese Traditional and Herbal Drugs, 2017, 48 (23): 5019- 5031. doi: 10.7501/j.issn.0253-2670.2017.23.031
9	陈晶晶, 李曼. 苯酚分子印迹聚合物的制备及吸附性能研究[J]. 武汉轻工大学学报, 2016, 35 (1): 53- 55. doi: 10.3969/j.issn.2095-7386.2016.01.012
	CHEN J J , LI M . Preparation and adsorption properties of phenol molecularly imprinted polymers[J]. Journal of Wuhan Polytechnic University, 2016, 35 (1): 53- 55. doi: 10.3969/j.issn.2095-7386.2016.01.012
10	MA Y , PAN G , ZHANG Y , et al. Comparative study of the molecularly imprinted polymers prepared by reversible addition fragmentation chain transfer "bulk" polymerization and traditional radical "bulk" polymerization[J]. Journal of Molecular Recognition, 2013, 26 (5): 240- 251. doi: 10.1002/jmr.2267
11	贾玉香, 朱若华, 陈惠敏. 安定分子印迹聚合物的制备及应用[J]. 分析测试学报, 2012, 31 (1): 15- 21. doi: 10.3969/j.issn.1004-4957.2012.01.003
	JIA Y X , ZHU R H , CHEN H M . Preparation and application of diazepam molecularly imprinted polymers[J]. Journal of Instrumental Analysis, 2012, 31 (1): 15- 21. doi: 10.3969/j.issn.1004-4957.2012.01.003
12	PRATIWI R , MEGANTARA S , RAHAYU D , et al. Comparison of bulk and precipitation polymerization method of synthesis molecular imprinted solid phase extraction for atenolol using methacrylic acid[J]. Journal of Young Pharmacists, 2018, 11 (1): 12- 16. doi: 10.5530/jyp.2019.11.3
13	廖辉, 金晨, 何玉琴, 等. 槲皮素分子印迹聚合物的合成及表征[J]. 中草药, 2017, 48 (8): 1537- 1543.
	LIAO H , JIN C , HE Y Q , et al. Preparation and characterization of quercetin molecularly imprinted polymer microspheres[J]. Chinese Traditional and Herbal Drugs, 2017, 48 (8): 1537- 1543.
14	马珍珍, 何金兴, 赵涛, 等. 基于皮克林乳液聚合四环素磁性分子印迹——生物炭微球的研制[J]. 食品与机械, 2020, 36 (5): 70- 75.
	MA Z Z , HE J X , ZHAO T , et al. Study on the preparation of tetracyline magnetic molecularly imprinted biochar microspheres based on Pickering emulsion polymerization[J]. Food & Machinery, 2020, 36 (5): 70- 75.
15	SONG L , HE J , CHEN N , et al. Combined biocompatible medium with molecularly imprinted polymers for determination of aflatoxins B1 in real sample[J]. Journal of Separation Science, 2019, 42 (24): 3679- 3687. doi: 10.1002/jssc.201900564
16	赵玲钰, 王姣姣, 高文惠. 三氟氯氰菊酯分子印迹固相萃取膜的制备及其在食品检测中的应用[J]. 化学通报, 2019, 82 (1): 80- 86.
	ZHAO L Y , WANG J J , GAO W H . Preparation of cyhalothrin molecularly imprinted solid-phase extraction membrane and its application in food detection[J]. Chemistry, 2019, 82 (1): 80- 86.
17	杨鑫, 张华, 董爱军, 等. 分子印迹技术在天然产物分离纯化中的应用[J]. 东北农业大学学报, 2011, 42 (8): 146- 153.
	YANG X , ZHANG H , DONG A J , et al. Application of molecular imprinting technology in separation and purification of active ingredients from natural products[J]. Journal of Northeast Agricultural University, 2011, 42 (8): 146- 153.
18	陈海燕. 分子印迹——固相萃取技术在食品和环境样品分析中的应用研究[D]. 长春: 吉林大学, 2014.
	CHEN H Y. Study on application of molecular imprinting as solid phase extraction in food and environmental samples analysis[D]. Changchun: Jilin University, 2014.
19	陈子龙, 杨鑫, 吴亚芬, 等. 虚拟模板分子印迹聚合物在天然产物分离中的研究进展[J]. 中国中药杂志, 2020, 45 (4): 809- 815.
	CHEN Z L , YANG X , WU Y F , et al. Research progress of dummy template molecularly imprinted polymers in separation of natural products[J]. China Journal of Chinese Materia Medica, 2020, 45 (4): 809- 815.
20	韩霜. 分子印迹技术及其研究进展[D]. 大庆: 东北石油大学, 2014.
	HAN S. Molecular imprinting technology and its research progress[D]. Daqing: Northeast Petroleum University, 2014.
21	肖淑娟. 利用分子印迹技术分离天然产物中有效成分[D]. 唐山: 华北理工大学, 2008.
	XIAO S J. Separation of active components from natural products by molecular imprinting technique[D]. Tangshan: North China University of Science and Technology, 2008.
22	赵琪. 分子印迹复合材料的制备及用于食品和环境样品中污染物快速检测的研究[D]. 长春: 吉林大学, 2015.
	ZHAO Q. Preparation of molecularly imprinted polymer and its application in rapid determination of contaminants in food and environmental samples[D]. Changchun: Jilin University, 2015.
23	丛姣姣, 罗静, 高雅涵, 等. 表面分子印迹研究进展[J]. 高分子通报, 2015, (5): 10- 20.
	CONG J J , LUO J , GAO Y H , et al. Research progress of surface molecular imprinting technique[J]. Chinese Polymer Bulletin, 2015, (5): 10- 20.
24	侯会卿, 苏黎明, 黄嫣嫣, 等. 表面分子印迹材料和技术在分离分析中的应用进展[J]. 色谱, 2016, 34 (12): 1206- 1214.
	HOU H Q , SU L M , HUANG Y Y , et al. Recent applications of surface molecularly imprinting materials and techniques for separation and analysis[J]. Chinese Journal of Chromatography, 2016, 34 (12): 1206- 1214.
25	ROY E , PATRA S , TIWARI A , et al. Introduction of selectivity and specificity to graphene using an inimitable combination of molecular imprinting and nanotechnology[J]. Biosensors and Bioelectronics, 2017, 89:234- 248. doi: 10.1016/j.bios.2016.02.056
26	CHEN X, YUAN Y Y, YAN H Y, et al. Selective, sensitive, and miniaturized analytical method based on molecularly imprinted graphene oxide composites for the determination of naphthalene-derived plant growth regulators in apples[J/OL]. Food Chemistry, 2021, 349: 128982[2021-04-20]. https://doi.org/10.1016/j.foodchem.2020.128982.
27	DEEPA J R, ANIRUDHAN T S, SOMAN G, et al. Electrochemical sensing of methylmalonic acid based on molecularly imprinted polymer modified with graphene oxide and gold nanoparticles[J/OL]. Microchemical Journal, 2020, 159: 105489[2021-04-20]. https://doi.org/10.1016/j.microc.2020.105489.
28	RUI C F , HE J , LI Y Y , et al. Selective extraction and enrichment of aflatoxins from food samples by mesoporous silica FDU-12 supported aflatoxins imprinted polymers based on surface molecularly imprinting technique[J]. Talanta(Oxford), 2019, 201:342- 349.
29	BONYADI S , GHANBARI K . Development of highly sensitive and selective sensor based on molecular imprinted polydopaminecoated silica nanoparticles for electrochemical determination of sunset yellow[J]. Microchemical Journal, 2021, 167:1- 12.
30	叶松龄, 黄佳佳, 罗林, 等. 碳点荧光探针的制备及其在食品分析中的应用[J]. 分析化学, 2017, 45 (10): 1571- 1581.
	YE S L , HUANG J J , LUO L , et al. Preparation of carbon dots and their application in food analysis as signal probe[J]. Chinese Journal of Analytical Chemistry, 2017, 45 (10): 1571- 1581.
31	ANSARI S, MASOUM S. Recent advances and future trends on molecularly imprinted polymer-based fluorescence sensors with luminescent carbon dots[J/OL]. Talanta, 2021, 223: 121411[2021-04-20]. http://doi.org/10.1016/j.talanta.2020.121411.
32	HU X L, ZHAO Y Q, DONG J Y, et al. A strong blue fluorescent nanoprobe based on Mg/N co-doped carbon dots coupled with molecularly imprinted polymer for ultrasensitive and highly selective detection of tetracycline in animal-derived foods[J/OL]. Sensors and Actuators B: Chemical, 2021, 338: 129809[2021-04-20]. http://doi.org/10.1016/j.snb.2021.129809.
33	AKINJOKUN A I, PETRIK L F, OGUNFOWOKAN A O, et al. Isolation and characterization of nanocrystalline cellulose from cocoa pod husk(CPH) biomass wastes[J/OL]. Heliyon, 2021, 7(4): e6680[2021-04-20]. http://doi.org/10.1016/j.heliyon.2021.e06680.
34	GANGULY P, SENGUPTA S, DAS P, et al. Valorization of food waste: Extraction of cellulose, lignin and their application in energy use and water treatment[J/OL]. Fuel, 2020, 280: 118581[2021-04-20]. http://doi.org/10.1016/j.fuel.2020.118581.
35	LI N, YANG H. Construction of natural polymeric imprinted materials and their applications in water treatment: A review[J/OL]. Journal of Hazardous Materials, 2021, 403: 123643[2021-04-20]. http://doi.org/10.1016/j.jhazmat.2020.123643.
36	CAO J S, SHEN C, WANG X Q, et al. A porous cellulose-based molecular imprinted polymer for specific recognition and enrichment of resveratrol[J/OL]. Carbohydrate Polymers, 2021, 251: 117026[2021-04-20]. http://doi.org/10.1016/j.carbpol.2020.117026.
37	XU X R, CHEN X, YANG L Y, et al. Film-like bacterial cellulose based molecularly imprinted materials for highly efficient recognition and adsorption of cresol isomers[J/OL]. Chemical Engineering Journal, 2020, 382: 123007[2021-04-20]. http://doi.org/10.1016/j.cej.2019.123007.
38	ZHANG H , LI Y Q , ZHENG D Y , et al. Bio-inspired construction of cellulose-based molecular imprinting membrane with selective recognition surface for paclitaxel separation[J]. Applied Surface Science, 2019, 466:244- 253. doi: 10.1016/j.apsusc.2018.10.038
39	刘军, 任汝全, 张燕如, 等. 五种黄酮类分子印迹分离技术研究及应用进展[J]. 化工进展, 2019, 38 (7): 3365- 3376.
	LIU J , REN R Q , ZHANG Y R , et al. Progress in research and application of five kinds of flavonoids molecular imprinting separation technique[J]. Chemical Industry and Engineering Progress, 2019, 38 (7): 3365- 3376.
40	SONG H J, ZHANG H P, HE Y L, et al. Novel bayberry-and-honeycomb-like magnetic surface molecularly imprinted polymers for the selective enrichment of rutin from Sophora japonica[J/OL]. Food Chemistry, 2021, 356: 129722[2021-04-20]. http://doi.org/10.1016/J.FOODCHEM.2021.129722.
41	CHENG Y, NIE J Y, LIU H D, et al. Synthesis and characterization of magnetic molecularly imprinted polymers for effective extraction and determination of kaempferol from apple samples[J/OL]. Journal of Chromatography A, 2020, 1630: 461531[2021-04-20]. http://doi.org/10.1016/j.chroma.2020.461531.
42	YOU Z Y, FU Y F, XIAO A P, et al. Magnetic molecularly imprinting polymers and reduced graphene oxide modified electrochemical sensor for the selective and sensitive determination of luteolin in natural extract[J/OL]. Arabian Journal of Chemistry, 2021, 14(3): 102990[2021-04-20]. http://doi.org/10.1016/j.arabjc.2021.102990.
43	DRAMOU P , ITATAHINE A , FIZIR M , et al. Preparation of novel molecularly imprinted magnetic graphene oxide and their application for quercetin determination[J]. Journal of Chromatography B, 2019, 1124:273- 283.
44	赵扬帆, 郑宝东. 植物多酚类物质及其功能学研究进展[J]. 福建轻纺, 2006, (11): 107- 110.
	ZHAO Y F , ZHENG B D . Research progress of plant polyphenol and its function[J]. The Light & Textle Industries of Fujian, 2006, (11): 107- 110.
45	何慧清, 叶静敏, 程杏安, 等. 分子印迹聚合物固相萃取花生根茎中白藜芦醇[J]. 仲恺农业工程学院学报, 2020, 33 (3): 1- 7.
	HE H Q , YE J M , CHENG X A , et al. Molecularly imprinted polymer solid phase extracts resveratrol from peanut rhizome[J]. Journal of ZhongKai University of Agriculture and Technology, 2020, 33 (3): 1- 7.
46	HOU X Y, GUO B L, TONG Y K, et al. Using self-polymerization synthesis of boronate-affinity hollow stannic oxide based fragment template molecularly imprinted polymers for the selective recognition of polyphenols[J/OL]. Journal of Chromatography A, 2020, 1612: 460631[2021-04-20]. http://doi.org/10.1016/j.chroma.2019.460631.
47	张军杰, 刘娜, 高原, 等. 分子印迹磁性纳米材料对石榴皮中没食子酸的选择性富集和检测[J]. 分析试验室, 2020, 39 (5): 566- 571.
	ZHANG J J , LIU N , GAO Y , et al. Molecularly imprinted magnetic nanomaterials for selective enrichment and detection of gallic acid in pomegranate rind[J]. Chinese Journal of Analysis Laboratory, 2020, 39 (5): 566- 571.
48	AN J Y, WANG L T, LYU M J, et al. An efficiency strategy for extraction and recovery of ellagic acid from waste chestnut shell and its biological activity evaluation[J/OL]. Microchemical Journal, 2021, 160: 105616[2021-04-20]. http://doi.org/10.1016/j.microc.2020.105616.
49	邹佳美, 平欲晖, 吴丽芳, 等. 鞣花酸分子印迹聚合物的合成及其性能研究[J]. 分析科学学报, 2021, 37 (3): 317- 322.
	ZOU J M , PING Y H , WU L F , et al. Synthesis and properties studies of ellagic acid molecularly imprinted polymers[J]. Journal of Analytical Science, 2021, 37 (3): 317- 322.
50	GAO Y, TANG Y H, GAO L Y, et al. Fabrication of acid-resistant imprinted layer on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle[J/OL]. Analytica Chimica Acta, 2021, 1161: 338475[2021-04-20]. http://doi.org/10.1016/j.aca.2021.338475.
51	汤喜兰, 刘建勋, 李磊. 中药有机酸类成分的药理作用及在心血管疾病的应用[J]. 中国实验方剂学杂志, 2012, 18 (5): 243- 246.
	TANG X L , LIU J X , LI L . Pharmacological effects of organic acids in Chinese herbs and its application in cardiovascular diseases[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18 (5): 243- 246.
52	余小芳, 曹逸非, 张珊珊, 等. 水杨酸分子印迹聚合物整体柱合成及其固相微萃取应用[J]. 湖北大学学报(自然科学版), 2021, 43 (2): 122- 129.
	YU X F , CAO Y F , ZHANG S S , et al. Synthesis and application of salicylic acid imprinted polymer monolithic column for solid phase microextraction[J]. Journal of Hubei University(Natural Science Edition), 2021, 43 (2): 122- 129.
53	XIONG X X, LI C, YANG X Y, et al. In situ grown TiO₂ nanorod arrays functionalized by molecularly imprinted polymers for salicylic acid recognition and detection[J/OL]. Journal of Electroanalytical Chemistry, 2020, 873: 114394[2021-04-20]. http://doi.org/10.1016/j.jelechem.2020.114394.
54	BUFFON E, STRADIOTTO N R. A molecularly imprinted polymer on reduced graphene oxide-gold nanoparticles modified screen-printed electrode for selective determination of ferulic acid in orange peels[J/OL]. Microchemical Journal, 2021, 167: 106339[2021-04-20]. http://doi.org/10.1016/j.microc.2021.106339.
55	王杰. 枇杷叶中熊果酸的提取及分离的研究[D]. 北京: 北京林业大学, 2016.
	WANG J. Study on extraction and separation of ursolic acid from Eriobotrya japonica leaves[D]. Beijing: Beijing Forestry University, 2016.
56	张林, 张朝凤, 许翔鸿, 等. 生物碱提取分离方法研究进展[J]. 亚太传统医药, 2017, 13 (15): 49- 50.
	ZHANG L , ZHANG C F , XU X H , et al. A review on the extraction and separation of alkaloids[J]. Asia-Pacific Traditional Medicine, 2017, 13 (15): 49- 50.
57	GHASEMI S , NEMATOLLAHZADEH A . Molecularly imprinted ultrafiltration polysulfone membrane with specific nano-cavities for selective separation and enrichment of paclitaxel from plant extract[J]. Reactive & Functional Polymers, 2018, 126:9- 19.
58	张丽, 缑浩, 刘海霞, 等. 拉巴乌头碱分子印迹微球的制备及其吸附性能[J]. 甘肃农业大学学报, 2019, 54 (3): 210- 219.
	ZHANG L , GOU H , LIU H X , et al. Preparation of labaaconitine molecularly imprinted polymer and its adsorption properties[J]. Journal of Gansu Agricultural University, 2019, 54 (3): 210- 219.
59	NESTORA S , MERLIER F , PROST E , et al. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer[J]. Journal of Chromatography A, 2016, 1465:47- 54.
60	袁新华, 刘敦舜, 刘怡, 等. 苦参碱分子印迹氢键型超高交联吸附树脂的制备及吸附性能[J]. 江苏大学学报(自然科学版), 2019, 40 (5): 585- 590.
	YUAN X H , LIU D S , LIU Y , et al. Preparation and adsorption properties of hydrogen-bonded molecular imprinting hypercrosslinked polymeric adsorbent for Matrine[J]. Journal of Jiangsu University(Natural Science Edition), 2019, 40 (5): 585- 590.
61	JI W H , XIE H K , ZHOU J , et al. Water-compatible molecularly imprinted polymers for selective solid phase extraction of dencichine from the aqueous extract of Panax notoginseng[J]. Journal of Chromatography B, 2016, 1008:225- 233.
62	SADEGH N, ASFARAM A, JAVADIAN H, et al. Ultrasound-assisted solid phase microextraction-HPLC method based on Fe₃O₄@SiO₂-NH₂-molecularly imprinted polymer magnetic nano-sorbent for rapid and efficient extraction of harmaline from Peganum harmala extract[J/OL]. Journal of Chromatography B, 2021, 1171: 122640[2021-04-20]. http://doi.org/10.1016/j.jchromb.2021.122640.
63	李红红. 银杏内酯B分子印迹模板的制备及初步应用[D]. 合肥: 合肥工业大学, 2020.
	LI H H. Preparation and preliminary application of molecular imprinting template of Ginkgolide-B[D]. Hefei: Hefei University of Technology, 2020.
64	LI H H , ZHANG W C , WU Z Y , et al. Theoretical design, preparation, and evaluation of Ginkgolide B molecularly imprinted polymers[J]. Journal of Separation Science, 2020, 43 (2): 514- 523.

聚合方法 aggregation method	优点 advantage	缺点 disadvantage
本体聚合 bulk polymerization	操作容易，设备要求低^[10]the operation is simple and easy, and the requirements for hardware equipment are not high^[10]	需后处理^[11]follow-up processing required ^[11]
沉淀聚合 precipitation polymerization	无需后处理，体系纯，特异性吸附强^[17]no follow-up processing required and the system without surfactants is more pure and has stronger specific adsorption^[17]	溶剂要求高，需求量大^[12]the solvent demand is large, and the solvent used needs to be able to dissolve all the substances participating in the reaction^[12]
乳液聚合 emulsion polymerization	无需后处理，重复利用率高，水溶性高^[18]no follow-up processing required, and the reuse rate is high; and the water-soluble compounds can be separated^[18]	配比复杂，特异性吸附弱^[19]the ratio of organic phase to water phase needs to be adjusted many times; the system has weak specific adsorption^[19]
悬浮聚合 suspension polymerization	无需后处理，材料粒径大小可控^[18]no follow-up processing required, and the particle size is controllable^[18]	制备繁琐，应用局限性大^[20]the preparation process is lengthy; the application is limited due to the influence of the solvent^[20]
原位聚合 in-situ polymerization	更适用于制作分子印迹色谱柱^[21]it is more suitable for making molecularly imprinted chromatographic columns^[21]	特异性吸附弱，重复利用率低^[20]the specific adsorption is not strong, and the reuse rate is low^[20]

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