银杏LCC制备多孔生物载体及在人体肝细胞培养中的应用

doi:10.3969/j.issn.0253-2417.2014.06.013

林产化学与工业 ›› 2014, Vol. 34 ›› Issue (6): 81-87.doi: 10.3969/j.issn.0253-2417.2014.06.013

银杏LCC制备多孔生物载体及在人体肝细胞培养中的应用

李金玲¹, 王鹏¹, 杨静¹, 谌凡更², 谢志杰³, 谢益民¹

1. 湖北工业大学制浆造纸工程学院, 湖北武汉 430068;
2. 华南理工大学制浆造纸工程国家重点实验室, 广东广州 510641;
3. 浙江省人民医院微创外科, 浙江杭州 310014

收稿日期:2014-02-28 出版日期:2014-12-25 发布日期:2015-08-18
通讯作者: 谢益民, 教授,博士生导师,主要从事植物纤维资源化学的研究工作;E-mail:ppymxie@scut.edu.cn. E-mail:ppymxie@scut.edu.cn
作者简介:李金玲(1988-),女,湖北襄阳人,硕士,研究方向:植物纤维资源化学
基金资助:
国家自然科学基金资助项目(31370574,31300494);湖北省教育厅青年人才项目(Q2013402)

Preparation of Porous Biological Carrier with Gingko LCC and Application in Culture of Human Hepatocytes

LI Jin-ling¹, WANG Peng¹, YANG Jing¹, CHEN Fan-geng², XIE Zhi-jie³, XIE Yi-min¹

1. School of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, China;
2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510641, China;
3. Minimally Invasive Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, China

Received:2014-02-28 Online:2014-12-25 Published:2015-08-18

摘要/Abstract

摘要： 以银杏木质素-碳水化合物复合体(LCC)为原料,通过洗脱支架的方法制备医用多孔生物载体,并利用红外光谱、液相色谱、核磁共振波谱及扫描电子显微镜对该生物载体的主要成分含量、化学结构以及表面形态进行分析.结果表明:以银杏LCC为原料可以制备性能优良的生物载体.该载体不仅含有愈创木基型苯丙烷结构单元,而且糖组分中对人体肝细胞有较高生物相容性的半乳糖和甘露糖分别占3.30%和21.94%.制备的多孔生物载体孔隙率可达60%以上,平均孔径约为130 μm.用多孔生物载体进行人体肝细胞培养,发现人体肝细胞能够成功地在银杏LCC多孔生物载体上生长,且细胞增殖较快,白蛋白分泌和葡萄糖代谢活性的测定结果表明肝细胞具有较高的代谢活性.研究结果表明银杏LCC多孔生物载体具有良好的生物相容性.

关键词: 木质素-碳水化合物复合体, 多孔载体, 人肝细胞, 培养, 生物相容性

Abstract: A porous biological carrier was prepared by the method of eluting stent with lignin-carbohydrate complexes (LCC) isolated from ginkgo wood. Fourier transform infrared spectrometer(FT-IR), high performance liquid chromatograph(HPLC), nuclear magnetic resonance (NMR) spectroscopy and scanning electron microscope (SEM) were applied to elucidate the composition, chemical structure and morphology of the biological carrier. The results indicate that a biological carrier with excellent performance can be prepared with gingko LCC as raw material. Not only the guaiacyl lignin subunits existe in this biological carrier, but also the galactose and mannose with high biological compatibility to human hepatocytes are found to be 3.30% and 21.94%, respectively. The porosity of prepared porous scaffold is more than 60% with average pore size of 130 μm. The carriers were used to culture human hepatocytes. The results show that human hepatocytes can grow successfully on carriers. Furthermore, the human hepatocytes on carriers proliferate rapidly and show a high metabolic activity by determination of human albumin and glucose metabolism. These interesting results indicate that porous biological carrier prepared with gingko LCC have an excellent biocompatibility.

Key words: lignin-carbohydrate complexes, porous carrier, human hepatocytes, culture, biocompability

中图分类号:

TQ35

李金玲, 王鹏, 杨静, 谌凡更, 谢志杰, 谢益民. 银杏LCC制备多孔生物载体及在人体肝细胞培养中的应用[J]. 林产化学与工业, 2014, 34(6): 81-87.

LI Jin-ling, WANG Peng, YANG Jing, CHEN Fan-geng, XIE Zhi-jie, XIE Yi-min. Preparation of Porous Biological Carrier with Gingko LCC and Application in Culture of Human Hepatocytes[J]. Chemistry and Industry of Forest Products, 2014, 34(6): 81-87.

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银杏LCC制备多孔生物载体及在人体肝细胞培养中的应用

Preparation of Porous Biological Carrier with Gingko LCC and Application in Culture of Human Hepatocytes

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