欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2008, Vol. 28 ›› Issue (04): 1-6.

• 论文 •    下一篇

李子果肉单宁结构及其抗氧化能力的研究

张亮亮1, 李敏1, 林鹏1,2, 林益明1,2   

  1. 1. 厦门大学生命科学学院, 福建厦门, 361005;2. 厦门大学湿地与生态工程研究中心, 福建厦门, 361005
  • 收稿日期:2007-08-17 修回日期:1900-01-01 出版日期:2008-08-30 发布日期:2008-08-30
  • 通讯作者: 林益明,博士生导师,研究领域:植物生态学;E-mail:linym@xmu.edu.cn。

Study on Structure of Tannin in Prunus salicina Lindl. Fruits and Its Antioxidant Activity

ZHANG Liang-liang1, LI Min1, LIN Peng1,2, LIN Yi-ming1,2   

  1. 1. School of Life Sciences, Xiamen University, Xiamen 361005, China;2. Research Centre for Wetlands and Ecological Engineering, Xiamen University, Xiamen 361005, China
  • Received:2007-08-17 Revised:1900-01-01 Online:2008-08-30 Published:2008-08-30

PDF (PC)

717

摘要： 对李子果肉的总酚含量与可溶性缩合单宁含量进行了测定,并利用基质辅助激光解吸附离子化飞行时间质谱(MALDI-TOFMS)联合核磁共振(NMR)技术详细研究了李子果肉单宁的类型、聚合度及平均分子质量.同时用二苯基苦基肼自由基(DPPH·)法研究了李子果肉单宁的抗氧化活性.结果表明:李子果肉中总酚质量分数为8.29%±1.30%,可溶性缩合单宁质量分数为1.43%±0.93%;构成该单宁的黄烷-3-醇结构单元主要是表儿茶酚,在化学结构上属于原花青定类型,且大部分聚合物的结构单元之间存在A型和B型2种连接方式,平均聚合度为5.3,平均分子质量为1583.7u;李子果肉单宁具有较高的自由基清除能力,其半数有效浓度(EC50)为57.98mg/L.

关键词: 李子, 多酚, 缩合单宁, 抗氧化

Abstract: Contents of total phenolics and extractable condensed tannins in Prunus salicina Lindl. fruits were determined. Type of condensed tannins, degree of polymerization and distribution of polymer were characterized by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) spectroscopy. The effects of tannins from P. salicina fruits on scavenging free radicals were determined by using 1,1-diphenyl-2-picryhydrazyl (DPPH·) free radical test. The results were showed as follows: total phenolics and extractable condensed tannins were 8.29%±1.30% and 1.43%±0.93%, respectively; Epicatechin was the basic units occurring in condensed tannins, A-type and B-type lin-kages were most common among the structural units of polymers. The average degree of polymerization was 5.3, and mean molecular weight was 1583.7u. EC50 value was 57.98mg/L by DPPH· scavenging test.

Key words: Prunus salicina Lindl., polyphenolics, condensed tannins, antioxidation activity

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3