杜香叶多糖的提取及抗氧化和抗肿瘤活性研究

doi:10.3969/j.issn.0253-2417.2017.05.018

林产化学与工业 ›› 2017, Vol. 37 ›› Issue (5): 133-138.doi: 10.3969/j.issn.0253-2417.2017.05.018

杜香叶多糖的提取及抗氧化和抗肿瘤活性研究

李雪, 王宏伟, 郑东然, 张鸿宇, 王英逵

东北林业大学生命科学学院, 黑龙江哈尔滨 150040

收稿日期:2017-02-03 出版日期:2017-10-25 发布日期:2017-10-30
通讯作者: 王宏伟,副教授,研究方向为生物活性物质的制备与应用;E-mail:84970486@qq.com。 E-mail:84970486@qq.com
作者简介:李雪(1994-),女,山东淄博人,本科生,研究方向为生物活性物质的制备与应用
基金资助:
国家级大学生创新训练项目资助（201510225154）

Extraction and Antioxidant, Antiproliferative Activities of Polysaccharide from Ledum palustre L. Leaves

LI Xue, WANG Hongwei, ZHENG Dongran, ZHANG Hongyu, WANG Yingkui

Life Science College, Northeast Forestry University, Harbin 150040, China

Received:2017-02-03 Online:2017-10-25 Published:2017-10-30

摘要/Abstract

摘要： 采用水提醇沉法从杜香（Ledum palustre L.）叶中提取得到多糖，通过硫酸苯酚比色法测定杜香叶多糖含量；在单因素试验的基础上进行正交试验优化提取条件，采用试剂盒法和化学实验法测定其体外总抗氧化活性以及DPPH·、·OH和H₂O₂清除能力；采用肝癌细胞HepG2为细胞模型，研究其对HepG2细胞的体外抗增殖活性。结果表明：杜香叶多糖提取的最佳条件为：液料比30：1（mL：g），提取温度80℃，提取时间3 h。在此条件下，多糖提取得率为7.86%±0.16%，纯度为90.06%。在质量浓度1~5 g/L范围内，杜香叶多糖体外抗氧化活性和抗癌细胞增殖活性均呈现出量效关系趋势。5 g/L杜香叶多糖总抗氧化能力为（10.27±0.2） U/mL，达到相同质量浓度的Vc总抗氧化活性的95%以上；各浓度杜香叶多糖DPPH·、·OH清除能力均达到相同质量浓度的Vc 90%以上，杜香叶多糖对H₂O₂清除能力在5 g/L时达到相同质量浓度的Vc 60%以上；当质量浓度为5 g/L时，杜香叶多糖对HepG2细胞增殖抑制率达57.41%±0.02%。

关键词: 杜香, 多糖, 抗氧化, 抗肿瘤

Abstract: The polysaccharide from Ledum palustre L. leaves was obtained by ethanol precipitation, and its content was determined by phenol-sulfuric acid assay. Based on the single-factor test, a orthogonal test was designed to optimize the extracting conditions.The total antioxidant activity and the scavenging effect on DPPH free radical, OH free radical and H₂O₂ by the polysaccharide from L. palustre leaves in vitro were determined by the kit method and chemical method. The antiproliferative activity was evaluated on human liver cancer HepG2 cells in vivo. The results showed that the best extracting conditions of the polysaccharide were:the liquid-solid ratio 30:1(v/w), extraction temperature 80℃ and extraction time 3 h.Under these conditions,the extraction yield of the polysaccharide was 7.86%±0.16% and the purity reached 90.06%. In the range of 1-5 g/L, the antioxidant and antiproliferative activities increased with an increase of mass concentration. At the mass concentration of 5 g/L, the total antioxidant activity was(10.27±0.2) U/mL and reached more than that of 95% Vc with the same mass concentration. The scavenging effects on DPPH free radical and OH free radical in different concentration reached more than those of 90% of Vc with the same mass concentration. The scavenging effect on H₂O₂ reached more than that of 60% Vc with the same mass concentration and the proliferation inhibitory rate of HepG2 cells was 57.41%±0.02% at the mass concentration of 5 g/L.

Key words: Ledum palustre L., polysaccharide, antioxidant, anti tumor

中图分类号:

TQ35
S789.4

李雪, 王宏伟, 郑东然, 张鸿宇, 王英逵. 杜香叶多糖的提取及抗氧化和抗肿瘤活性研究[J]. 林产化学与工业, 2017, 37(5): 133-138.

LI Xue, WANG Hongwei, ZHENG Dongran, ZHANG Hongyu, WANG Yingkui. Extraction and Antioxidant, Antiproliferative Activities of Polysaccharide from Ledum palustre L. Leaves[J]. Chemistry and Industry of Forest Products, 2017, 37(5): 133-138.

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杜香叶多糖的提取及抗氧化和抗肿瘤活性研究

Extraction and Antioxidant, Antiproliferative Activities of Polysaccharide from Ledum palustre L. Leaves

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