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林产化学与工业

林产化学与工业 ›› 2009, Vol. 29 ›› Issue (1): 46-50.

• 研究报告 • 上一篇    下一篇

桦木酮酸对人肝癌细胞及对动物移植性肿瘤H22细胞的影响

张秀娟1,2, 李国媛1,2, 季宇彬1,2, 方桂珍3   

  1. 1. 哈尔滨商业大学, 生命科学与环境科学研究中心, 黑龙江 哈尔滨, 150076;2. 哈尔滨商业大学, 药物研究所抗肿瘤天然药物教育部工程研究中心, 黑龙江 哈尔滨, 150076;3. 东北林业大学, 材料工程与技术学院, 黑龙江 哈尔滨, 150040
  • 收稿日期:2008-01-15 修回日期:1900-01-01 出版日期:2009-02-28 发布日期:2009-02-28

Influences of Betulonic Acid on HepG-2 Cell and Mice Bearing H22 Tumor Cells

ZHANG Xiu-juan1,2, LI Guo-yuan1,2, JI Yu-bin1,2, FANG Gui-Zhen3   

  1. 1. Center of Research & Development on Life Science and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China;2. Engineering Research Center of Anti-tumor Natural Drug, Ministry of Education, Harbin University of Commerce, Harbin 150076, China;3. College of Material and Engineering, Northeast Forestry University, Harbin 150040, China
  • Received:2008-01-15 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28

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摘要： 研究桦木酮酸体内、体外抗肿瘤作用,探讨桦木酮酸抗肿瘤作用机制。体内研究观察桦木酮酸对H22荷瘤小鼠生命延长率的影响,体外实验采用溴化四氮唑蓝(MTT)法观察桦木酮酸对人肝癌细胞(HepG-2)的抑制作用;电子显微镜观察桦木酮酸对H22荷瘤小鼠肿瘤细胞凋亡现象并采用流式细胞仪检测其对肿瘤细胞周期的影响。结果表明桦木酮酸对小鼠腹水型肝癌H22有一定抑制作用,可显著延长H22荷瘤小鼠的生存时间,体外可抑制HepG-2的生长,半数抑制浓度(IC50)为110.77μmol/L,半数毒性浓度(TC50)为683.57μmol/L。说明桦木酮酸在体内、体外均表现出良好的肿瘤抑制作用。电子显微镜下可见肿瘤细胞体积明显变小,表面微绒毛脱失,细胞核固缩,细胞核内染色质形成固缩,核膜结构清晰,细胞器膜结构轻微改变。流式细胞仪检测结果发现:随剂量的增加,H22细胞S期和G2期的含量逐渐增多,故可推测桦木酮酸作用于细胞时,可把细胞阻滞于S期和G2期。说明桦木酮酸对肿瘤细胞的作用与促凋亡作用有关。

关键词: 桦木酮酸, 肿瘤细胞, 细胞凋亡, 细胞周期

Abstract: The anti-tumor effect of betulonic acid in vivo and in vitro and the preliminary mechanism of action were studied.The survival time of mice bearing H22 was used to observe the potency in vitro and the effect of betulonic acid on human hepatoma cell line(HepG-2) in vitro was studied. The morphology of H22 tumor cells treated with betulonic acid was observed by transmission electron microscopy.The effect of betulonic acid on the cell cycle was adopted by flow cytometry. The results showed that betulonic acid had certain effect on survival time of mice in vitro and HepG-2 in vitro. In the experiments of tumor transplantation in vivo, it could be observed that betulonic acid had some therapeutic effect on H22, it could significantly extend the survival time of the mice vaccinated by H22. This showed that betulonic acid also showed good tumor inhibition in vivo experiments. The values of IC50 and TC50 are 110.77 and 683.57μmol/L, respectively.The apoptosis of tumour cells can be observed by electronmicroscopy. Therefore, betulonic acid had anti-tumor effect by promoting tumor cellapoptosis.Increasing the dosage of betulonic acid, H22 cells in S phase and G2 phase gradually increased. It can be speculated that when betulonic acid acted on cells, the cells were blocked in S and G2 phases.

Key words: betulonic acid, tumour cells, apoptosis, cell cycle

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