辐照预处理水稻秸秆在高底物浓度下的酶解

doi:10.3969/j.issn.0253-2417.2015.02.020

Abstract

Abstract: The effect of high dose irradiation on polysaccharides of rice straw was studied. The feasibility of the achievement of high-concentration sugar by the way of improving substrate concentration through fed-batch enzymatic hydrolysis process of irradiated rice straw, was then investigated. The result showed that the polysaccharides content of irradiated rice straw decreased and the water-soluble sugar increased from 0.7% to 13.4%. By the way of fed-batch enzymatic hydrolysis, the solid loading could reach 40% (mass fraction) in the enzymatic hydrolysis system. And 104.6 g/L glucose concentration as well as 77.1% cellulose conversion were obtained by this process under the condition of 32.8 FUP/g (calculated by cellulose) cellulase loading.

Key words: irradiation, rice straw, high solid loading, enzymatic hydrolysis

CLC Number:

TQ35

CHEN Liang, SU Xiao-jun, CHEN Jing-ping, WU Xiao-fen, XIONG Xing-yao, HU Qiu-long, WANG Ke-qin. Enzymatic Hydrolysis of Rice Straw Pretreated by Irradiation at High Solid Loading[J]. Chemistry and Industry of Forest Products, 2015, 35(2): 129-134.

References

[1] GOLDEMBERG J.Ethanol for a sustainable energy future[J].Science, 2007, 315(5813):808-810.
[2] ZHAO Xue-bing, SONG Yuan-quan, LIU De-hua .Enzymatic hydrolysis and simultaneous saccharification and fermentation of alkali/peracetic acid-pretreated sugarcane bagasse for ethanol and 2, 3-butanediol production[J].Enzyme and Microbial Technology, 2011, 49(4):413-419.
[3] RUDOLF A, ALKASRAWI M, ZACCHI G, et al.A comparison between batch and fed-batch simultaneous saccharification and fermentation of steam pretreated spruce[J].Enzyme and Microbial Technology, 2005, 37(2):195-204.
[4] YANG Mao-hua, LI Wang-liang, LIU Bin-bin, et al.High-concentration sugars production from corn stover based on combined pretreatments and fed-batch process[J].Bioresource Technology, 2010, 101(13):4884-4888.
[5] JIN Seop-bak, JA Kyong-ko, YOUNG Hwan-han.Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment[J].Bioresource Technology, 2009, 100(3):1285-1290.
[6] 王洁瑛.热处理及γ射线辐射杉木压缩木材的固定[D].北京:北京林业大学博士学位论文, 2000. WANG Jie-ying.Fixation of compressed wood of Chinese fir treated with heat or Gamma rays irradiation[D].Beijing:Doctoral Dissertation of Beijing Forestry University, 2000.
[7] 孙丰波.竹材Co⁶⁰射线辐照效应及其机理研究[D].北京:中国林业科学研究院博士学位论文, 2010. SUN Feng-bo.Study on irradiation effects and mechanism in bamboo treated with Co⁶⁰ Gamma rays[D].Beijing:Doctoral Dissertation of Chinese Academy of Forestry, 2010.
[8] WANG Ke-qin, XIONG Xing-yao, CHEN Jing-ping, et al .Effect of ⁶⁰Co γ irradiation on the microcrystalline cellulose of Phragmites communis Trin.[J].Wood and Fiber Science, 2011, 43(2):225-231.
[9] 陈静萍, 王克勤, 熊兴耀, 等.Co⁶⁰γ辐照对稻草纤维组织及酶解效果的影响[J].核农学报, 2008, 22(3):304-309. CHEN Jing-ping, WANG Ke-qin, XIONG Xing-yao, et al.Effect of Co⁶⁰γ irradiation on rice straw fiber structure and enzyme hydrolyzation[J].Journal of Nuclear Agricultural Sciences, 2008, 22(3):304-309.
[10] 胡婷春, 杨青丹, 张婷婷, 等.辐照-纤维素酶糖化降解芒草工艺条件优化研究[J].中国酿造, 2011(2):29-32. HU Ting-chun, YANG Qin-dan, ZHANG Ting-ting, et al.Optimal conditions of irradiation-cellulase saccharification technology of Chinese silvergrass[J].China Brewing, 2011(2):29-32.
[11] YANG Chun-ping, SHEN Zhi-qiang, YU Guo-ce, et al.Effect and aftereffect of γ radiation pretreatment on enzymatic hydrolysis of wheat straw[J].Bioresource Technology, 2008, 99(14):6240-6245.
[12] 石淑兰, 何福望.制浆造纸分析与检测[M].北京:中国轻工业出版社, 2006. SHI Shu-lan, HE Fu-wang.Pulping & Paper-marking Amalysis[M].Beijing:China Light Industry Press, 2006.
[13] 陈亮, 陈静萍, 罗志平, 等.预处理过的稻草在两种不同溶液中酶解性能的比较[J].可再生能源, 2014, 32(9):1379-1384. CHEN Liang, CHEN Jing-ping, LUO Zhi-ping, et al.Comparative enzymatic digestibility of pretreated straw in two different kinds of solution[J].Renewable Energy Resources, 2014, 3(9):1379-1384.
[14] GHOSE T K.Measurement of cellulase activities[J].Pure &Applied Chemistry, 1987, 59(2):257-268.
[15] ZHU Li, O'DWYER J P, CHANG V S, et al.Structural features affecting biomass enzymatic digestibility[J].Bioresource Technology, 2008, 99(9):3817-3828.
[16] 张志萍, 周雪花, 岳建芝, 等.超微玉米芯粉的酶解预处理工艺实验研究[J].太阳能学报, 2013, 34(9):1536-1541. ZHANG Zhi-ping, ZHOU Xue-hua, YUE Jian-zhi, et al.Enzymatic hydrolysis pretreatment of ultra-corncob powder[J].Acta Energiae Solaris Sinica, 2013, 34(9):1536-1541.
[17] 刘超纲, 刘力, 余世袁.分批添料纤维素酶水解研究[J].林产化学与工业, 1996, 16(1):58-62. LIU Chao-gang, LIU Li, YU Shi-yuan.Study on fed-batch enzymatic hydrolysis of cellulose[J].Chemistry and Industry of Forest Products, 1996, 16(1):58-62.
[18] CARRASCO C, BAUDEL H M, SENDELIUS J, et al.SO₂-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse[J].Enzyme and Microbial Technology, 2010, 46(2):64-73.
[19] TORNY E, JOHAN B, FOLKE T.Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose[J].Enzyme and Microbial Technology, 2002, 31(3):353-364.
[20] LI Hui, KIM Nag-jong, JIANG Min, et al.Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid-acetone for bioethanol production[J].Bioresource Technology, 2009, 100(13):3245-3251.

Enzymatic Hydrolysis of Rice Straw Pretreated by Irradiation at High Solid Loading

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