樟树叶精油组成分析及抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2020.01.012

Abstract

Abstract:

Four different chemical types of camphor leaves essential oils were extracted by steam distillation and analyzed by gas chromatography-mass spectrometry to compare their compositional differences. The antioxidant activity of camphora leaves essential oil was evaluated by DPPH, ABTS and FRAP, and butylated hydroxyl anisole (BHA) was used as positive control. The results showed that 46 kinds of main chemical components were detected in four chemical types of camphor oils. Among them, 35 kinds of chemical components were detected in the isonerolidol type oil, and the main component was isonerolidol, which was 29.48%. A total of 33 chemical components were detected in linalool type oil, and the main component was linalool, which was 71.91%. A total of 33 chemical components were detected in camphor type oil, and the main component was camphor, which was 46.28%. A total of 33 chemical components were detected in cineol type oil, and the main components was 1, 8-cineole, which was 30.31%. The four chemical types of camphora oils all exhibited a certain scavenging effects on DPPH radical, ABTS radical and reducing power in a concentration-dependent manner. But their antioxidant capacity was lower than BHA.Among them, camphor type oil exhibited the strongest of DPPH and scavenging ability and Fe³⁺ reducing power. When the concentration was 8 g/L, the DPPH radical scavenging rate of camphor type oil was 30.34%±1.25%, FRAP value was (1.44±0.13) mmol/L. isonerolidol type oil showed the strongest ABTS radical scavenging ability. When the concentration was 8 g/L, the ABTS radical scavenging rate of isonerolidol type oil was 14.93%±2.32%.

Key words: camphora leaves essential oil, gas chromatography-mass spectrometry, antioxidant activity

CLC Number:

TQ35

Jiaxin LI,Binbin MENG,Kai ZHU. Components and Antioxidant Activity of Camphor Leaves Essential Oil[J]. Chemistry and Industry of Forest Products, 2020, 40(1): 84-90.

Figures/Tables 4

Fig.1

Table 1

Chemical groups of different camphor leaves essential oil"

编号 No.	化合物名称 compounds name	保留时间/min retention time	GC含量 GC content/%
编号 No.	化合物名称 compounds name	保留时间/min retention time	芳樟 linalool	脑樟 camphor	异樟 isonerolidol	油樟 cineol
1	4-甲基-2-戊酮4-methyl-2-pentanone	3.80	0.70	—	—	—
2	正己醛hexanal	4.58	0.05	0.05	0.10	0.04
3	二丙酮醇diacetone alcohol	5.21	0.05	0.05	—	0.03
4	反式-3-己烯-1-醇cis-3-hexen-1-ol	5.43	0.36	0.36	—	0.14
5	叶醇leaf alcohol	5.44	—	—	0.23	—
6	α-蒎烯α-pinene	6.68	1.11	7.19	2.34	6.09
7	莰烯camphene	6.95	0.59	4.07	1.30	0.36
8	桧烯sabinene	7.34	0.35	1.29	0.15	15.52
9	β-蒎烯β-pinene	7.43	0.43	2.26	1.15	4.33
10	月桂烯myrcene	7.57	0.29	2.59	0.64	1.88
11	水芹烯α-phellandrene	7.88	—	0.62	0.58	0.08
12	对伞花烃p-cymene	8.26	—	—	—	0.14
13	(+)-柠檬烯(+)-dipentene	8.33	—	5.18	0.77	—
14	1, 8-桉叶油素1, 8-cineole	8.40	1.48	4.61	0.63	30.31
15	罗勒烯α-ocimene	8.62	0.37	0.10	0.21	0.15
16	松油烯α-terpinene	8.89	0.08	0.35	—	1.65
17	顺-α, α-5-三甲基-5-乙烯基四氢化呋喃-2-甲醇cis-α, α, 5-trimethyl-5-vinyltetrahydrofuran-2-methanol	9.16	0.70	—	—	—
18	萜品油烯terpinolene	9.49	—	0.52	—	1.45
19	芳樟醇linalool	9.70	71.91	3.04	10.97	1.42
20	3-辛烯-2-醇3-octen-2-ol	10.72	4.22	—	—	—
21	樟脑camphor	10.81	—	46.28	2.80	5.57
22	异龙脑isoborneol	11.21	0.16	0.96	0.23	1.28
23	(-)-4-萜品醇(-)-terpinen-4-ol	11.44	0.22	0.98	0.11	3.43
24	α-松油醇α-terpineol	11.72	0.60	1.25	0.70	11.27
25	(R)-3, 7-二甲基-6-辛烯醇(R)-(+)-β-citronellol	12.44	—	—	0.55	—
26	左旋乙酸冰片酯L-bornyl acetate	13.86	0.40	1.67	—	0.06
27	(-)-α-荜澄茄油烯(-)-α-cubebene	15.39	0.12	—	—	—
28	β-波旁烯β-bourbonene	16.26	—	0.14	0.15	—
29	β-榄香烯β-elemene	16.33	0.38	0.51	0.30	0.81
30	反式石竹烯caryophyllene	16.90	2.79	2.55	5.48	2.46
31	(+)-香橙烯(+)-aromandendrene	17.21	0.19	0.19	0.44	0.12
32	α-律草烯α-caryophyllene	17.26	0.69	3.48	2.22	0.82
33	香树烯aromadendrene	17.53	0.05	0.05	0.11	0.03
34	大根香叶烯germacrene D	17.79	0.73	1.82	2.32	1.26
35	β-瑟林烯β-selinene	17.87	1.30	0.18	0.15	2.16
36	牛儿烯bicyclogermacrene	17.99	4.24	4.07	6.49	3.53
37	杜松烯cadinene	18.26	0.17	0.22	0.25	0.20
38	水杨酸salicylic acid	18.49	—	—	0.15	—
39	榄香醇elemol	18.58	—	—	—	0.28
40	异橙花叔醇isonerolidol	18.67	1.01	—	29.48	—
41	桉油烯醇spathulenol	18.93	1.17	0.42	11.42	0.22
42	石竹素caryophyllene oxide	19.00	0.49	0.20	0.25	0.18
43	环氧化蛇麻烯humulene epoxide	19.25	0.07	0.08	0.68	0.03
44	蓝桉醇(-)-globulol	19.59	—	—	0.41	—
45	异香橙烯环氧化物isoaromadendrene epoxide	19.64	—	—	0.73	—
46	蒿酮3, 3, 6-trimethylhepta-1, 5-dien-4-one	20.11	—	—	9.70	—

Table 1

Table 2

Fig.2

References 18

1	程必强, 喻学俭, 丁靖垲. 中国樟属植物资源及其芳香成分[M]. 昆明: 云南科技出版社, 1997.
	CHENG B Q , YU X Y , DING J W . Cinnamomum Plant Resources and Their Aromatic Constituents in China[M]. Kunming: Yunnan Science and Technology Press, 1997.
2	王中生. 樟科观赏树种资源及园林应用[J]. 中国野生植物资源, 2001, 20 (4): 31- 33. doi: 10.3969/j.issn.1006-9690.2001.04.012
	WANG Z S . Ornamental tree species resources and garden application in polygonaceae[J]. Chinese Wild Plant Resources, 2001, 20 (4): 31- 33. doi: 10.3969/j.issn.1006-9690.2001.04.012
3	PINO J A , FUENTES V . Leaf oil of Cinnamomum camphora(L.) J.Presl.from Cuba[J]. Journal of Essential Oil Research, 1998, 10 (5): 531- 532. doi: 10.1080/10412905.1998.9700962
4	石皖阳, 何伟, 文光裕. 樟精油成分和类型划分[J]. 植物学报, 1989, (3): 47- 52.
	SHI Y Y , HE W , WEN G Y . Classification and composition of essential oils[J]. Journal of Integrative Plant Biology, 1989, (3): 47- 52.
5	温朋飞, 彭艳. 植物精油抗氧化作用机制研究进展[J]. 饲料工业, 2017, (2): 44- 49.
	WEN P F , PENG Y . Research progress on antioxidant mechanism of plant essential oils[J]. Feed Industry, 2017, (2): 44- 49.
6	SOSA V , MOLINÉ T , SOMOZA R , et al. Oxidative stress and cancer:An overview[J]. Ageing Research Reviews, 2013, 12 (1): 376- 390. doi: 10.1016/j.arr.2012.10.004
7	DONG H , ZHANG Q , LI L , et al. Antioxidant activity and chemical compositions of essential oil and ethanol extract of Chuan minshen violaceum[J]. Industrial Crops and Products, 2015, 76, 290- 297. doi: 10.1016/j.indcrop.2015.04.051
8	张艳东, 程翠林, 樊梓鸾, 等. 植物精油的提取、成分分析以及抗氧化活性研究[J]. 食品工业科技, 2017, (3): 348- 352.
	ZHANG Y D , CHENG C L , FAN W , et al. Extraction, component analysis and antioxidant activity of essential oils from plants[J]. Science and Technology of Food Industry, 2017, (3): 348- 352.
9	黄婷.四种樟属植物精油的成分分析、抗氧化活性及对中华按蚊幼虫的毒杀活性测定[D].重庆:重庆师范大学, 2016.
	HUANG T.Composition analysis, antioxidant activity and toxicity of Anopheles sinensis larvae of four Eucalyptus essential oils[D]. Chongqing: Chongqing Normal University 2016.
10	VALKO M , RHODES C J , MONCOL J , et al. Free radicals, metals and antioxidants in oxidative stress-induced cancer[J]. Chemico-Biological Interactions, 2006, 160 (1): 1- 40. doi: 10.1016/j.cbi.2005.12.009
11	GVLCIN I . Antioxidant activity of food constituents:An overview[J]. Archives of Toxicology, 2012, 86 (3): 345- 391. doi: 10.1007/s00204-011-0774-2
12	REGNAULT-ROGER C , VINCENT C , ARNASON J T . Essential oils in insect control:Low-risk products in a high-stakes world[J]. Annual Review of Entomology, 2012, 57 (1): 405- 424. doi: 10.1146/annurev-ento-120710-100554
13	OMP S , TEJK B . DPPH antioxidant assay revisited[J]. Food Chemistry, 2009, 113 (4): 1202- 1205. doi: 10.1016/j.foodchem.2008.08.008
14	FLOEGEL A , KIM D O , CHUNG S J , et al. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods[J]. Journal of Food Composition&Analysis, 2011, 24 (7): 1043- 1048.
15	BENZIE I F , STRAIN J J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power":The FRAP assay[J]. Analytical Biochemistry, 1996, 239 (1): 70- 76. doi: 10.1006/abio.1996.0292
16	何晋浙, 沈强. 猴头菌菌丝体萜类物质提取优化及抗氧化研究[J]. 浙江工业大学学报, 2016, (3): 326- 333. doi: 10.3969/j.issn.1006-4303.2016.03.018
	HE J Z , SHEN Q . Extraction optimization and antioxidation of aphid from Mycelium of Hericium erinaceus[J]. Journal of Zhejiang University of Technology, 2016, (3): 326- 333. doi: 10.3969/j.issn.1006-4303.2016.03.018
17	章佳妮, 侯建平, 翁新楚. 酚类抗氧化机理的进一步探讨[J]. 粮油加工, 2009, (4): 58- 63.
	ZHANG J N , HOU J P , WENG X C . Further discussion on antioxidant mechanism of phenols[J]. Cereals and Oils Processing, 2009, (4): 58- 63.
18	周凌云, 鲁斌, 余小红, 等. 隐孔菌中倍半萜类化合物及其抗氧化活性研究[J]. 林产化学与工业, 2018, 38 (1): 77- 84.
	ZHOU L Y , LU B , YU X H , et al. Study on sesquiterpenoids in cryptic bacteria and their antioxidant activity[J]. Chemistry and Industry of Forest Products, 2018, 38 (1): 77- 84.

种类 kinds	质量浓度/(g·L^-1) concentration	DPPH·清除率/% DPPH·scavenging rate	·ABTS⁺清除率/% ·ABTS⁺scavenging rate
	0.5	0.27	3.79
	1.0	3.36	4.35
芳樟linalool	2.0	4.03	5.44
	4.0	12.48	6.69
	8.0	23.09	9.49
	0.5	0.40	3.92
	1.0	2.82	4.67
脑樟camphor	2.0	11.68	5.44
	4.0	18.79	7.86
	8.0	30.34	14.15
	0.5	2.15	3.68
	1.0	2.42	5.29
异樟isonerolidol	2.0	3.36	7.00
	4.0	9.53	9.43
	8.0	16.24	14.93
	0.5	1.61	3.20
	1.0	3.09	4.04
油樟cineol	2.0	5.23	4.82
	4.0	17.05	5.29
	8.0	19.87	7.15
	0.5	75.34	21.22
	1.0	81.20	31.26
BHA	2.0	83.09	58.95
	4.0	84.29	90.02
	8.0	85.44	96.31

[1]	Zijiang LI,Wei LIU,Lei WU,Dan WANG,Chuanling SI. Monosaccharide Composition and Biological Activity of Polysaccharides from Pine Cones of Pinus pumila (Pall.) Regel [J]. Chemistry and Industry of Forest Products, 2020, 40(1): 37-44.
[2]	Jie WANG,Jiaojiao LU,Han DU,Meng ZHANG,Panpan LIU,Xiaoqiang CHEN. Comparative Study on Essential Oils of Schisandra chinensis Fruits Pulp and Seeds: Chemical Composition and Bioactivities [J]. Chemistry and Industry of Forest Products, 2019, 39(6): 59-67.
[3]	Huifang ZHANG,Ye WANG,Weichao HU,Shu YUAN,Ming YUAN. Purification and Antioxidation Activity of Polyphenol from Cyrtomium fortune [J]. Chemistry and Industry of Forest Products, 2019, 39(2): 81-88.
[4]	JIANG Ping, ZHANG Qian, ZHAO Yajie, LU Tingting, WANG Fei, ZHANG Liping. Extraction Technology for Total Polyphenols from Eucalyptus grandis×E. urograndis Bark and Their Bioactivities [J]. Chemistry and Industry of Forest Products, 2018, 38(5): 37-44.
[5]	WANG Xiang, PENG Sheng, PENG Mijun. Extraction of Total Polyphenols from Eucommia ulmoides Oliver Leaves Assisted by Ultrasound-microwave and Their Antioxidant Activity [J]. Chemistry and Industry of Forest Products, 2018, 38(5): 85-92.
[6]	LIU Dandan, HAN Weifan, JIANG Jianxin, ZHU Liwei. Technology of Microwave-assisted Extraction of Sapindus Peel Polysaccharide and Its Antioxidant Activity [J]. Chemistry and Industry of Forest Products, 2018, 38(4): 109-116.
[7]	JIANG Ping, CHEN Huafang, HUANG Haibin, BAO Xinye, XU Lan. Ultrasonic-assisted Extraction of Total Flavonoids from Branches and Leaves of Taxus cuspidata Sieb. et Zucc. and Its Antioxidant Activity [J]. Chemistry and Industry of Forest Products, 2017, 37(6): 125-132.
[8]	ZHOU Meng, LIU Gongjun, HE Lingxiao, WANG Fei. Preparation and Antioxidant Activity of Proanthocyanidins Dimers from Acacia mearnsii De Willd [J]. Chemistry and Industry of Forest Products, 2017, 37(2): 135-140.
[9]	LI Yan, AN Li-ping, GUO Yu-chen, JIN Li-e. Synthesis and Antioxidant Acticity of Stearoyl Tannic Acid Ester Based on Hydroxyl Protection [J]. Chemistry and Industry of Forest Products, 2016, 36(6): 49-55.
[10]	XU Xiao-xiang, YIN Xiao-li, HONG Yan-ping, ZOU Kai, SHANGGUAN Xin-chen, LIANG Qiang. Extraction Process and Antioxidant Activity of Pinus taeda L. Polysaccharides [J]. Chemistry and Industry of Forest Products, 2016, 36(3): 114-120.
[11]	KUANG Chun-tao KUANG Chun-tao, LI Xiang-zhou, XUE Hai-peng, DENG Nan, WANG Ling-zhi. Synthesis and Biological Activity of Curcumin Derivatives [J]. Chemistry and Industry of Forest Products, 2014, 34(5): 67-72.
[12]	LU Sheng-lou, FU Jia-zhen, LIU Hong, FU Chun-min, LIN You-ming, WANG Xiao-he. Ultrasonic Extraction and Antioxidant Activity of Polyphenol from Synsepalum dulcificum Leaf [J]. Chemistry and Industry of Forest Products, 2014, 34(5): 145-151.
[13]	WANG Ying-ying;XIONG Shuang-li;SHI Min-juan;YAO Xiao-lei. Biological Activities of Acidic Polysaccharide and Its Derivatives from Prunella vulgaris Linn. [J]. , 2013, 33(5): 35-40.
[14]	WAN Chun-peng;ZHOU Shou-ran. Chemical Constituents of Acer rubrum L. and Their Antioxidant Activities [J]. , 2013, 33(5): 93-96.
[15]	WANG Hui;ZENG Yi-cheng;HOU Ying;WENG Rui-jie;YANG Jing-jin;ZHU Yong;ZHOU Wen-zhong;YANG Yan. Analysis of Different Volatile Constituents in the Wood Material by Headspace Solid Phase Microextraction-gas Chromatography-mass Spectrometry [J]. , 2012, 32(5): 115-119.

Components and Antioxidant Activity of Camphor Leaves Essential Oil

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 18

Related Articles 15

Recommended Articles

Metrics

Comments