HPLC-DAD法分析不同月份和干燥方式下银杏叶色素含量的变化规律

doi:10.3969/j.issn.0253-2417.2023.02.012

Abstract

Abstract:

HPLC-DAD method was used to study the change law of content of Ginkgo biloba leaves pigments in different months, and the effects of various drying methods on the content of G.biloba leaves pigments were evaluated by cluster analysis and technique for order preference by similarity to an ideal solution(TOPSIS). The results showed that the established HPLC-DAD method was simple, accurate, sensitive and reproducible. G. biloba leaves pigments content was significantly different from April to November. The contents of chlorophylide a, pheophorbidel a and β-carotene reached the highest values in April, which were (1 762±121), (628±32) and (482±45) μg/g, respectively. The contents of chlorophyll a and b arrived at the highest values in May, which were (8 701±571) and(3 140±274) μg/g, respectively. The content of lutein could get to the highest value in July, which was (2 057±104) μg/g. Cluster analysis results demonstrated that 12 kinds of drying methods could be divided into four categories. Freeze drying, vacuum drying at 40℃, and atmospheric drying at 40℃ were clustered as A category. Shade drying was clustered as B category. vacuum drying at 100℃ and atmospheric drying at 100℃ were clustered as D category. The left 6 kinds of drying methods were clustered as C category. Through the analysis of TOPSIS method, it was found that the top five drying methods of G. biloba leaves were freeze drying, vacuum drying at 40℃, atmospheric drying at 40℃, shade drying and atmospheric drying at 60℃. Considering comprehensively, vacuum drying at 40℃ was the most suitable drying method for industrial production, with fast drying speed and effective protection of pigments.

Key words: HPLC-DAD, Ginkgo biloba leaves, pigments, drying modes, change law

CLC Number:

TQ35
TQ041

Hong SHEN, Changwei ZHANG, Hongxia CHEN, Hua YUAN, Jianxin JIANG, Chengzhang WANG. Change Law of Pigments Content of Ginkgo biloba Leaves in the Different Months and Dry Modes Analyzed by HPLC-DAD Method[J]. Chemistry and Industry of Forest Products, 2023, 43(2): 89-97.

Figures/Tables 7

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References 22

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编号 No.	干燥方式¹⁾ drying methods	压强/kPa pressure	温度/℃ temperature	时间/h time
S1		-100	40	6
S2	减压干燥 vacuum drying	-100	60	4
S3		-100	80	2
S4		-100	100	1
S5			40	12
S6	常压干燥 atmospheric drying		60	4
S7			80	2
S8			100	1
S9	冷冻干燥freeze drying		-50	24
S10	杀青减压干燥^* blanching vacuum drying	-100	60	4
S11	晒干sun drying		25-35	12
S12	阴干shade drying		25-30	120

序号 No.	组分 component	回归方程 regression equation	相关系数 correlation coefficient	线性范围/ (mg·L^-1) linear range	检出限/ (mg·L^-1) LOD	定量限/ (mg·L^-1) LOQ
1	叶绿素a chlorophyll a	Y=73 697X+90 179	0.999 2	4.688-75	0.156	0.468
2	脱镁叶绿素a pheophytin a	Y=125 106X+78 017	0.999 4	2.5-40	0.133	0.400
3	脱植基叶绿素a chlorophyllide a	Y=69 897X+20 103	0.999 2	0.625-10	0.042	0.125
4	脱镁叶绿酸盐a pheophorbide a	Y=110 345X+1 282	0.999 6	1.563-25	0.052	0.156
5	叶绿素b chlorophyll b	Y=114 304X+89 782	0.999 4	1.563-25	0.052	0.156
6	脱镁叶绿素b pheophytin b	Y=170 481X+4 866	0.999 9	1.25-20	0.042	0.125
7	叶黄素lutein	Y=86 820X+10 182	0.999 7	1.563-25	0.052	0.156
8	α-胡萝卜素 α-carotene	Y=130 310X+10 627	0.999 3	0.625-10	0.042	0.125
9	β-胡萝卜素 β-carotene	Y=118 310X+10 627	0.999 8	0.625-10	0.068	0.246

月份 month	脱植基叶绿素a chlorophyllide a	脱镁叶绿酸盐a pheophorbide a	叶绿素b chlorophyll b	叶绿素a chlorophyll a	脱镁叶绿素a pheophytin a	叶黄素 lutein	α-胡萝卜素 α-carotene	β-胡萝卜素 β-carotene
4月Apr.	1 762±121	628±32	1 745±141	3 875±315	725±43	872±49	56±5	482±45
5月May	616±37	509±41	3 140±274	8 701±571	474±26	1 201±68	272±39	336±21
6月June	410±26	352±17	2 904±157	7 958±446	479±45	1 251±101	331±27	328±11
7月July	—	168±85	2 231±152	5 528±321	484±43	2 057±104	349±25	315±27
8月Aug.	—	159±12	1 696±154	3 974±378	486±41	865±83	332±31	314±32
9月Sep.	—	147±12	1 025±101	2 303±231	562±54	553±53	132±12	222±21
10月Oct.	—	—	1 005±98	2 194±212	803±78	467±43	86±7	218±20
11月Nov.	—	—	109±9	217±21	50±4	364±34	55±4	91±8

编号 No.	脱植基叶绿素a chlorophyllide a	脱镁叶绿酸盐a pheophorbide a	叶绿素b chlorophyll b	叶绿素a chlorophyll a	脱镁叶绿素b pheophytin b	脱镁叶绿素a pheophytin a	叶黄素 lutein	α-胡萝卜素 α-carotene	β-胡萝卜素 β-carotene
S1	120±11	305±29	2 840±281	7 010±689	216±23	2 120±213	1 256±126	319±30	312±31
S2	—	287±27	1 025±101	1 165±112	1 944±191	6 074±603	898±87	263±25	250±23
S3	—	283±28	1 175±115	1 014±104	1 374±132	6 207±621	685±69	256±25	269±26
S4	—	189±19	501±46	426±43	848±79	3 335±321	278±28	113±12	124±13
S5	98±8	301±28	2 645±261	6 819±687	126±12	2 111±209	1 056±106	301±32	309±31
S6	—	293±27	1 037±101	1 342±132	1 968±187	6 203±632	924±93	283±27	282±27
S7	—	280±26	1 165±112	1 004±98	1 270±124	6 107±613	585±57	205±21	224±23
S8	—	180±15	478±34	435±42	809±78	3 039±301	250±26	97±11	76±8
S9	280±23	330±32	2 904±278	8 012±802	—	479±48	1 253±123	330±32	319±32
S10	—	75±6	—	—	2 020±201	6 068±609	703±71	55±5	63±6
S11	—	273±25	1 236±121	1 345±132	1 975±178	6 009±589	889±89	270±28	265±27
S12	—	295±27	1 624±15	3 300±331	1 567±154	4 045±408	1 114±113	295±30	281±27

编号 No.	正理想解距离 positive ideal solution distance(D⁺)	负理想解距离 negative ideal solution distance(D^-)	相对接近度 relative proximity(C_i)	排序结果 ranking results
S1	0.681	0.972	0.588	2
S2	1.080	0.692	0.390	7
S3	1.097	0.619	0.361	8
S4	1.247	0.299	0.194	11
S5	0.746	0.890	0.544	3
S6	1.072	0.715	0.400	5
S7	1.113	0.568	0.338	9
S8	1.264	0.271	0.176	12
S9	0.547	1.298	0.704	1
S10	1.268	0.561	0.307	10
S11	1.064	0.705	0.399	6
S12	0.990	0.716	0.420	4

Change Law of Pigments Content of Ginkgo biloba Leaves in the Different Months and Dry Modes Analyzed by HPLC-DAD Method

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