超声波辅助类芬顿预处理增强桉木酶解效果

doi:10.3969/j.issn.0253-2417.2023.02.013

Abstract

Abstract:

To overcome problems in traditional Fenton pretreatment, an ultrasonic-assisted iron-loaded zeolite Fenton-like(UZF) system was constructed, and single factor method was used to determine optimal conditions for UZF pretreatment of eucalyptus, i.e., H₂O₂ concentration of 4%, ultrasonic power of 240 W, and treatment time of 90 min. Under these conditions, the reducing sugar produced by enzymatic enzymolysis of eucalyptus powder was as high as (435.12±7.69) mg/g. The mechanism of UZF pretreatment to enhance enzymolysis efficiency of eucalyptus was investigated by means of composition, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy(SEM). The changes of main components and groups, crystalline properties, and surface microstructure of eucalyptus samples before and after UZF pretreatment were analyzed. The results showed that pretreatment removed more lignin and hemicellulose, which increased destruction of amorphous area of cellulose, and surface holes of wood powder became more and surface was more rough. The experimental results of recycling showed that the iron-loaded zeolite had certain recycling ability. After four times of recycling, reducing sugar yield could still reach 1.87 times than that of untreated eucalyptus powder.

Key words: ultrasonic, iron-loaded zeolite, fenton-like, eucalyptus, enzymolysis

CLC Number:

TQ35

Mengjie CHEN, Zhinan WANG, Shujie WANG, Yingshi HUANG, Xianfeng HOU, Zhenzhong GAO. Ultrasound-assisted Fenton-like Pretreatment of Eucalyptus for Enhancing Enzymolysis[J]. Chemistry and Industry of Forest Products, 2023, 43(2): 98-104, 126.

Figures/Tables 7

Table 1

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References 29

1	KOSTIC S , BERG J K , CASDORFF K , et al. A straightforward thiol-ene click reaction to modify lignocellulosic scaffolds in water[J]. Green Chemistry, 2017, 19 (17): 4017- 4022. doi: 10.1039/C7GC01601H
2	解先利, 刘云云, 曹运齐, 等. 木质纤维素酶水解分形动力学的研究进展[J]. 新能源进展, 2021, 9 (5): 426- 433. doi: 10.3969/j.issn.2095-560X.2021.05.009
	XIE X L , LIU Y Y , CAO Y Q , et al. Research progress on fractal dynamics of enzymatic hydrolysis of lignocellulose[J]. Advances in New and Renewable Energy, 2021, (5): 426- 433. doi: 10.3969/j.issn.2095-560X.2021.05.009
3	SOLTANIAN S, AGHBASHLO M, ALMASI F, et al.A critical review of the effects of pretreatment methods on the exergetic aspects of lignocellulosic biofuels[J/OL]. Energy Conversion and Management, 2020, 212: 112792[2022-01-10]. https://doi.org/10.1016/j.enconman.2020.112792.
4	黄元, 王志敏, 张宏森, 等. 芬顿反应用于木质纤维素生物质预处理的研究现状[J]. 纤维素科学与技术, 2018, 26 (4): 68- 75.
	HUANG Y , WANG Z M , ZHANG H S , et al. Progress of Fenton reaction for pretreatment of lignocellulosic biomass[J]. Journal of Cellulose Science and Technology, 2018, 26 (4): 68- 75.
5	LIU W, WU R J, HU Y Y, et al.Improving enzymatic hydrolysis of mechanically refined poplar branches with assistance of hydrothermal and Fenton pretreatment[J/OL]. Bioresource Technology, 2020, 316: 123920[2022-01-10]. https://doi.org/10.1016/j.biortech.2020.123920.
6	ZENG J , CHANG G Y , FEI W , et al. Biomimetic Fenton-catalyzed lignin depolymerization to high-value aromatics and dicarboxylic acids[J]. ChemSusChem, 2015, 8 (5): 861- 871. doi: 10.1002/cssc.201403128
7	KATO D M , ELIA N , FLYTHE M , et al. Pretreatment of lignocellulosic biomass using Fenton chemistry[J]. Bioresource Technology, 2014, 162, 273- 278. doi: 10.1016/j.biortech.2014.03.151
8	刘庆玉, 杨明, 张敏, 等. 低过氧化氢浓度的芬顿试剂预处理玉米秸秆试验[J]. 沈阳农业大学学报, 2019, 50 (5): 628- 633.
	LIU Q Y , YANG M , ZHANG M , et al. Experimental of pretreatment of corn stover with Fenton reagent with low hydrogen peroxide concentration[J]. Journal of Shenyang Agricultural University, 2019, 50 (5): 628- 633.
9	HEGNAR O A , GOODELL B , FELBY C , et al. Challenges and opportunities in mimicking non-enzymatic brown-rot decay mechanisms for pretreatment of Norway spruce[J]. Wood Science and Technology, 2019, 53 (2): 291- 311. doi: 10.1007/s00226-019-01076-1
10	PEDERSEN M , JOHANSEN K S , MEYER A S . Low temperature lignocellulose pretreatment: Effects and interactions of pretreatment pH are critical for maximizing enzymatic monosaccharide yields from wheat straw[J]. Biotechnology for Biofuels, 2011, 4 (1): 1- 10. doi: 10.1186/1754-6834-4-1
11	GARRIDO-RAMIREZ E G , THENG B K G , MORA M L . Clays and oxide minerals as catalysts and nanocatalysts in Fenton-like reactions: A review[J]. Applied Clay Science, 2010, 47 (3/4): 182- 192.
12	WANG Z M, ZHANG F J, LYU D C, et al.Iron oxychloride-based heterogeneous Fenton pretreatment of corn stover for enhanced sugars production[J/OL]. Chemical Engineering Journal, 2020, 416: 127703[2022-01-10]. https://doi.org/10.1016/j.cej.2020.127703.
13	李得钊, 胡芳, 许秀葵, 等. 超声波强化木质纤维素预处理的研究进展[J]. 纤维素科学与技术, 2020, 28 (1): 69- 77.
	LI D Z , HU F , XU X K , et al. Progress of ultrasound intensification for lignocellulose pretreatment[J]. Journal of Cellulose Science and Technology, 2020, 28 (1): 69- 77.
14	TAMURA H , GOTO K , YOTSUYANAGI T , et al. Spectrophotometric determination of iron(Ⅱ) with 1, 10-phenanthroline in the presence of large amounts of iron(Ⅲ)[J]. Talanta, 1974, 21 (4): 314- 318. doi: 10.1016/0039-9140(74)80012-3
15	XIAO K , YUAN Y , XIAO N D , et al. Enzymatic saccharification responses of Eichhornia crassipes, sugarcane bagasse and Metasequoia glyptostroboides to two oxidation pretreatments for biofuel production[J]. Industrial Crops & Products, 2017, 107, 22- 29.
16	VAN SOEST P J , ROBERTSON J B , LEWIS B A . Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science, 1991, 74 (10): 3583- 3597. doi: 10.3168/jds.S0022-0302(91)78551-2
17	李大群, 蒋进元, 周岳溪, 等. 载铁沸石催化H₂O₂降解阳离子红X-GRL染料废水[J]. 环境工程技术学报, 2013, 3 (5): 391- 397. doi: 10.3969/j.issn.1674-991X.2013.05.061
	LI D Q , JIANG J Y , ZHOU Y X , et al. Degradation of cationic red X-GRL dye wastewater with H₂O₂ catalyzed by Fe-containing zeolite[J]. Journal of Environmental Engineering Technology, 2013, 3 (5): 391- 397. doi: 10.3969/j.issn.1674-991X.2013.05.061
18	ZHANG Y T, LIANG J, ZHOU W B, et al.Comparison of Fenton and bismuth ferrite Fenton-like pretreatments of sugarcane bagasse to enhance enzymatic saccharification[J/OL]. Bioresource Technology, 2019, 285: 121343[2022-01-10]. https://doi.org/10.1016/j.biortech.2019.121343.
19	LAI L W , IDRIS A . Comparison of steam-alkali-chemical and microwave-alkali pretreatment for enhancing the enzymatic saccharification of oil palm trunk[J]. Renewable Energy, 2016, 99, 738- 746. doi: 10.1016/j.renene.2016.07.059
20	MOHAPATRA S , PADHY S , DAS MOHAPATRA P K , et al. Enhanced reducing sugar production by saccharification of lignocellulosic biomass, Pennisetum species through cellulase from a newly isolated Aspergillus fumigatus[J]. Bioresource Technology, 2018, 253, 262- 272.
21	李坚. 木材波谱学[M]. 北京: 科学出版社, 2003.
	LI J . Wood Spectroscopy[M]. Beijing: Science Press, 2003.
22	PANDEY K K , PITMAN A J . FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi[J]. International Biodeterioration & Biodegradation, 2003, 52 (3): 151- 160.
23	YANG L, RU Y, XU S, et al.Features correlated to improved enzymatic digestibility of corn stover subjected to alkaline hydrogen peroxide pretreatment[J/OL]. Bioresource Technology, 2021, 325(4): 124688[2022-01-10]. https://doi.org/10.1016/j.biortech.2021.124688.
24	WU K , WU H , ZHANG H Y , et al. Enhancing levoglucosan production from waste biomass pyrolysis by Fenton pretreatment[J]. Waste Management, 2020, 108, 70- 77.
25	GAO C F, YANG J, HAN L J.Systematic comparison for effects of different scale mechanical-NaOH coupling treatments on lignocellulosic components, micromorphology and cellulose crystal structure of wheat straw[J/OL]. Bioresource Technology, 2021, 326: 124786[2022-01-10]. https://doi.org/10.1016/j.biortech.2021.124786.
26	DAI L, HUANG T, JIANG K K, et al.A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose[J/OL]. Biotechnology for Biofuels, 2021, 14: 35[2022-01-10]. https://doi.org/10.1186/s13068-021-01884-3.
27	HALDAR D, PURKAIT M K.A review on the environment-friendly emerging techniques for pretreatment of lignocellulosic biomass: Mechanistic insight and advancements[J/OL]. Chemosphere, 2021, 264: 128523[2022-01-10]. https://doi.org/10.1016/j.chemosphere.2020.128523.
28	WANG S J , OUYANG X H , WANG W Y , et al. Comparison of ultrasound-assisted Fenton reaction and dilute acid-catalysed steam explosion pretreatment of corncobs: cellulose characteristics and enzymatic saccharification[J]. RSC Advances, 2016, 6 (80): 76848- 76854.
29	LI X, CUI K, GUO Z, et al.Heterogeneous Fenton-like degradation of tetracyclines using porous magnetic chitosan microspheres as an efficient catalyst compared with two preparation methods[J/OL]. Chemical Engineering Journal, 2020, 379: 122324[2022-01-10]. https://doi.org/10.1016/j.cej.2019.122324.

样品sample	O	Al	Si	Ca	Fe
天然沸石natural zeolite	65.22	6.38	26.32	1.75	0.32
载铁沸石iron-loaded zeolite	58.89	10.52	28.35	0.38	1.87

条件conditions		还原糖产量/(mg·g^-1) reducing sugar yield
H₂O₂用量 H₂O₂ dosage	2%	421.10
	4%	435.12
	6%	373.33
超声波功率 ultrasonic power	160 W	391.00
	240 W	435.12
	320 W	365.25
时间 time	50 min	411.45
	90 min	435.12
	130 min	293.37

桉木木粉¹⁾eucalyptus powder	纤维素cellulose/%	半纤维素hemicellulose/%	木质素lignin/%
未处理untreated	41.35±1.61	26.31±2.22	27.79±0.70
UAF预处理UAF pretreated	44.98±0.70	22.41±3.20	27.03±0.45
UZF预处理UZF pretreated	45.52±0.99	25.32±0.55	23.15±1.11

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Ultrasound-assisted Fenton-like Pretreatment of Eucalyptus for Enhancing Enzymolysis

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