Abstract: Acanthopanax sessiliflorus (Rupr. et Maxim.) Seem. has been used instead of A.senticosus in northeast region of China,but its active components are not very clear. In this article,the constituents of flavonoids and polysaccharides(ASP) from A.sessiliflorus fruit were studied by TLC and HPLC respectively. Using microemulsion as mobile phase on polyamide plate, nine constituents were separated from the flavonoids.R_f analysis showed one constituents to be hyperin, while rutin and quercetin were not found. The polysaccharides(ASP) were composed of Glc,Gal,Man,Fuc,Xyl,Rha,GlcN,GlcUA,GalUA and were mainly composed of four homogeneous polysaccharides whose molecular weights (M_r) are 21800,147000,45400,18100 respectively. Among them, the homogeneous polysaccharide ASP-Ⅰ was consisted of Gal and Rha in the molar ratio of 1:0.7,with M_r of 18100.The animal tests showed that ASP can increase the survival time under hypoxia condition and loaded swimming time of mice.

Key words: Acanthopanax sessiliflorus( Rupr. et Maxim.)Seem., polysaccharide, total flavonoids