Abstract: Supercritical CO₂ extraction(SCDE) is a kind of effective comprehensive extraction for antioxidant and essential oil of rosemary(Rosmarinus officinalis L.). In this study, the optimal conditions of comprehensive extraction for antioxidant and essential oil of rosemary were as follows: extraction time 4 h, 95%(volume fraction) ethanol as entrainer to rosemary leaves 2:5, temperature of extraction pot 50℃, temperature of separation pot I 70℃, temperature of separation pot Ⅱ 40℃, extraction pressure 20 MPa and separation pressure 4-5 MPa. Average yield of rosemary oil was 1.80%. Results of constituent analysis of rosemary oil showed that the essential oil mainly contains 1,8-cineole, camphor, α-terpineol, borneol, verbenone, terpinen-4-ol, bornyl acetate and linalol. Average yield of rosemary antioxidant was 11.93%. Main active components of rosemary antioxidant were carnosic acid, carnosol, and rosmarinic acid, whose contents were 23.61%, 7.33% and 5.13%, respectively.

Key words: rosemary(Rosmarinus officinalis L.), antioxidant, essential oil, supercritical CO₂ extraction