Abstract: Adsorption of rubusoside from Rubus suavissimus S. Lee by 6 kinds of macroporous resins were tested to obtain the best adsorbent. Some of factors, not only on adsorption of rubusoside including the concentration of rubusoside, pH value and flow rate of solution, but also on desorption such as selection of desorbents and temperature, were investigated. The results showed that AB-8 resin was the best adsorbent for adsorption of rubusoside from R.suavissimus among 6 kinds of macroporous resins. The optimum conditions for adsorption by AB-8 resin were as follows: rubusoside solution concentration 7.7mg/L, pH value 8 and flow rate 3 BV/h. For desorption, ethanol solution showed good performance and temperature had obvious influence so both processes concerned were gained to complete desorption of rubusoside from adsorbent. One process can be operated under the conditions of 70% ethanol solution as desorbent, flow rate 3 BV/h(BV means the resin bed volume on chromatography column), total desorbent 5 BV at room temperature, or under the conditions of 60% ethanol solution as desorbent, flow rate 3 BV/h, total desorbent 4 BV at 40℃. Rubusoside was obtained successfully from R. suavissimus by this process in our laboratory.

Key words: macroporous adsorption resin, extraction, rubusoside