Abstract: Flavonoids were extracted from seabuckthorn (Hippophae rhamnoides Linn.)residue by cellulase-assisted technique. The extracting condition was optimized using response surface analysis based on single factor experiments. A mathematical model was established and analyzed by response surface methodology(RSM) to describe the relationships among mass ratio of cellulase,ratio of solutions to material,extraction temperature,pH value and extraction yield of flavonoids. The optimal flavonoids extraction conditions were:dosage of cellulase 78.9IU/g,ratio of solutions to material 25.9,temperature 59.1℃ and pH value 3.9.Under the optimized extraction conditions, flavonoids yield is (8.48±0.16)mg/g(n=3),which is close to the estimated value 8.57mg/g attained from regression model.This model can be used to predict the experiment results well.

Key words: seabuckthorn residue, flavonoids, cellulase, mathematical model, surface methodology