Abstract: Using propyl gallate as standard material, the optimum conditions for determining the content of phenolic compounds in bamboo vinegar by Folin-Ciocalteu's method were investigated. The results showed that the reaction conditions were:ratio of Folin reagent to 10% Na₂CO₃ 1:1(volume ratio ),reaction temperature 35℃, reaction time 120 min. There was a good linearity in range of 5-30 g/L.The average recovery rate was 101.55% and the relative standard deviation (RSD) was 0.30%. This method had a good accuracy and precision to determine the content of phenolic compounds.

Key words: bamboo vinegar, phenolic compounds, Folin-Ciocalteu's method