漆树木粉抗氧化活性成分的HPLC-MS分析

doi:10.3969/j.issn.0253-2417.2017.01.012

Abstract

Abstract: The ethanol extract from Rhus verniciflua Stokes wood powder was partitioned with different polarity solvents to obtain petroleum ether fraction, ethyl acetate fraction, n-butylalcohol fraction and water fraction. The DPPH·, ABTS· and OH· scavenging activities of the four fractions were evaluated. The results showed that the extraction yield of ethyl acetate was the highest (60.1%) and its scavenging activities of DPPH·, ABTS· and OH· were the strangest with the IC₅₀ of 19.9, 29.74 and 37.95 mg/L, respectively. Twelve compounds in ethyl acetate fraction were identified by HPLC-MS, including six phenolic acids, i.e., ethoxy-3-hydroxy benzoic acid(1), gallic acid(2), 3,4-dihydroxy almond acid(3), gallic acid cetyl ester(4), protocatechuic acid(5) and ethyl gallate(7) and six flavonoids, i.e., fustin(6), 3,4',7-trihydroxy-flavanonol(9), fisetin(13), sulfuretin(14), butin(15) and 3,7-dihydroxy flavanone-4'-rhamnose(16).

Key words: Rhus verniciflua Stokes, wood powder, antioxidant, active component, HPLC-MS

CLC Number:

TQ35

CHEN Hongxia, WANG Chengzhang, ZHOU Hao, TAO Ran, YE Jianzhong. HPLC-MS Analysis of Antioxidant Components from Rhus verniciflua Stokes Wood Powder[J]. Chemistry and Industry of Forest Products, 2017, 37(1): 94-100.

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HPLC-MS Analysis of Antioxidant Components from Rhus verniciflua Stokes Wood Powder

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