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›› 2008, Vol. 28 ›› Issue (04): 115-117.

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Preliminary Study on Chemical Structure of Pine Nut Kernel Polysaccharide

LIU Rong1, SUN Fang2, CHEN Xiu-li1, WANG Xiang-hong3   

  1. 1. College of Forestry, Northeast Forestry University, Harbin 150040, China;2. Mongolian Textile Industry College, Chifeng 024000, China;3. Heilongjiang Province Natural Resource Research Institute, Harbin 150040, China
  • Received:2007-08-27 Revised:1900-01-01 Online:2008-08-30 Published:2008-08-30

Abstract: Four polysaccharide fractions (PNP1-a,PNP1-b,PNP2-a,PNP3-a) were isolated from the kernel of Pinus koraiensis Sieb.et Zucc by hot water extraction, ethanol precipitation and purification followed by DEAE-cellulose and gel-permeation column chromatographies. The molecular weights of the 4 fractions were 10.2×104, 1.43×104, 11.0×104 and 16.3×104 respectively. The unit monosaccharides of PNP1-a were identified as rhamnose and glucose at the ratio of 1.00:2.57; and the unit monosaccharide of PNP1-b was identified as arabinose and glucose at the ratio of 1.00:4.03; PNP2-a and PNP3-a were both consisted of glucose. The structures of the fractions were analysed through FT-IR, ultraviolet and NMR spectroscopies, the results showed that monosaccharides of PNP1-a, PNP1-b, PNP2-a and PNP3-a were all of β-pyranose configuration.

Key words: pine nut kernel polysaccharide, structure, property

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