响应面法优化超声波辅助乙醇/盐双水相萃取栀子中京尼平苷

doi:10.3969/j.issn.0253-2417.2017.02.012

林产化学与工业 ›› 2017, Vol. 37 ›› Issue (2): 93-100.doi: 10.3969/j.issn.0253-2417.2017.02.012

响应面法优化超声波辅助乙醇/盐双水相萃取栀子中京尼平苷

刘磊磊^1,2, 赵帅¹, 王欢¹, 彭胜^1,2, 郭婕^1,2

1. 吉首大学林产化工工程湖南省重点实验室, 湖南张家界 427000;
2. 湖南省植物功能成分利用协同创新中心, 湖南张家界 427000

收稿日期:2016-06-27 出版日期:2017-04-25 发布日期:2017-05-04
作者简介:刘磊磊(1984-),男,河南汝州人,讲师,博士,主要从事天然产物化学与分离新方法研究;E-mail:liull126@126.com
基金资助:
国家自然科学基金资助项目（31560105）；湖南省自然科学基金资助项目（14JJ6037）

Optimization of Ultrasonic Assisted Extraction of Geniposide from Gardenia Fruits by Using Ethanol/Salt Aqueous Two-phase System with Response Surface Method

LIU Leilei^1,2, ZHAO Shuai¹, WANG Huan¹, PENG Sheng^1,2, GUO Jie^1,2

1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China;
2. Hunan Co-innovation Center for Utilization of Botanical Functional Ingredients, Zhangjiajie 427000, China

Received:2016-06-27 Online:2017-04-25 Published:2017-05-04

摘要/Abstract

摘要： 采用超声波辅助乙醇/盐双水相萃取技术对栀子中京尼平苷的提取分离效果进行了系统研究，在单因素试验基础上，采用响应曲面试验设计进行优化，进一步比较了该方法与超声波溶剂提取法、热回流提取法等传统方法对京尼平苷的提取效果。结果表明：超声波辅助乙醇/盐双水相萃取的最佳工艺条件为构建10.0 g乙醇/NaH₂PO₄双水相体系，其中加入无水乙醇4 mL（即体系中乙醇质量分数为31.56%），NaH₂PO₄质量分数为15.00%，超声波温度为30 ℃，超声波时间为30 min，栀子干粉加入量为0.03 g，上相中京尼平苷的得率为51.77 mg/g。该方法提取时间短、提取温度较低，而且得率较高，对栀子中京尼平苷的提取效果明显优于超声波溶剂提取法和热回流提取法等传统方法。

关键词: 双水相体系, 超声波辅助, 栀子, 京尼平苷

Abstract: An ultrasonic-assisted technology combined with ethanol/NaH₂PO₄ aqueous two-phase system (UAATPE) was developed for the extraction and purification of geniposide (GP) from gardenia fruits. Based on the results of single factor experiments, the Box-Behnken Design combined with response surface methodology was applied to optimize the operating parameters. Furthermore, the comparisons with ultrasonic solvent extraction, heat reflux extraction, and ultrasonic-assisted aqueous two-phase extraction were also assessed. GP was mainly extracted into the top ethanol-rich phase. And the optimal experimental conditions were 10.0 g ethanol/salt aqueous two-phase system, NaH₂PO₄ mass fraction 15.00%, ethanol 4 mL(ethanol mass fraction 31.56%), extraction temperature 30 ℃, extraction time 30 min and sample loading 0.03 g. And the maximum yield of GP in top phase was 51.77 mg/g. The comparison results indicated that ultrasonic-assisted aqueous two-phase extraction was more efficient and environmentally friendly than ultrasonic solvent extraction and heat reflux extraction, for the technique had the shorter extraction time, the lower extraction temperature and the higher extraction efficiency.

Key words: aqueous two-phase systems, ultrasonic assisted, gardenia fruits, geniposide

中图分类号:

TQ35

刘磊磊, 赵帅, 王欢, 彭胜, 郭婕. 响应面法优化超声波辅助乙醇/盐双水相萃取栀子中京尼平苷[J]. 林产化学与工业, 2017, 37(2): 93-100.

LIU Leilei, ZHAO Shuai, WANG Huan, PENG Sheng, GUO Jie. Optimization of Ultrasonic Assisted Extraction of Geniposide from Gardenia Fruits by Using Ethanol/Salt Aqueous Two-phase System with Response Surface Method[J]. Chemistry and Industry of Forest Products, 2017, 37(2): 93-100.

参考文献

[1] 中华人民共和国药典委员会. 中华人民共和国药典:第一部[M]. 北京:中国医药科技出版社, 2015:248. Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China:Volume Ⅰ[M]. Beijing:China Medicine Science and Technology Press, 2015:248.
[2] TANG W C, EISENBRAND G. Chinese Drugs of Plant Origin-Gardenia jasminoides Eillis[M]. Berlin:Springer-Verlag, 1992:539-543.
[3] CHANG W L, WANG H Y, SHI L S, et al. Immunosuppressive iridoids from the fruits of Gardenia jasminoides[J]. Journal of Natural Products, 2005, 68(11):1683-1685.
[4] DENG Y H, GUAN M F, XIE X X, et al. Geniposide inhibits airway inflammation and hyperresponsiveness in a mouse model of asthma[J]. International Immunopharmacology, 2013, 17(3):561-567.
[5] CHEN J Y, WU H, LI H, et al. Anti-inflammatory effects and pharmacokinetics study of geniposide on rats with adjuvant arthritis[J]. International Immunopharmacology, 2015, 24(1):102-109.
[6] FU Y H, LIU B, LIU J H, et al. Geniposide, from Gardenia jasminoides Ellis, inhibits the inflammatory response in the primary mouse macrophages and mouse models[J]. International Immunopharmacology, 2012, 14(4):792-798.
[7] WANG G F, WU S Y, XU W, et al. Geniposide inhibits high glucose-induced cell adhesion through the NF-κB signaling pathway in human umbilical vein endothelial cells[J]. Acta Pharmacologica Sinica, 2010, 31(8):953-962.
[8] WANG C J, WANG S W, LIN J K. Suppressive effect of geniposide on the hepatotoxicity and hepatic DNA binding of aflatoxin B1 in rats[J]. Cancer Letters, 1991, 60(2):95-102.
[9] 郑礼胜, 倪娜, 刘向前, 等. 京尼平苷和京尼平研究及应用现状[J]. 药物评价研究, 2012, 35(4):289-298. ZHENG L S, NI N, LIU X Q, et al. Study and application of geniposide and genipin[J]. Drug Evaluation Research, 2012, 35(4):289-298.
[10] RATANAPONGLEKA K. Recovery of biological products in aqueous two phase systems[J]. International Journal of Chemical Engineering and Applications, 2010, 1(2):191-198.
[11] ALBERTSSON P A. Partition of Cell Particles and Macromolecules[M]. New York:Wiley, 1986.
[12] FEDERICO R R, JORGE B, OSCAR A, et al. Aqueous two-phase affinity partitioning systems:Current applications and trends[J]. Journal of Chromatography A, 2012, 1244:1-13.
[13] 刘磊磊, 李秀娜, 赵帅. 双水相萃取在中药活性成分提取分离中的应用进展[J]. 中草药, 2015, 46(5):766-773. LIU L L, LI X N, ZHAO S. Advances in application of aqueous two-phase system in separation and purification of active constituents from Chinese materia medica[J]. Chinese Traditional Herb Drugs, 2015, 46(5):766-773.
[14] PAN I H, CHIU H H, LU C H, et al. Aqueous two-phase extraction as an effective tool for isolation of geniposide from gardenia fruit[J]. Journal of Chromatography A, 2002, 977(2):239-246.
[15] MA F Y, GU C B, LI C Y, et al. Microwave-assisted aqueous two-phase extraction of isoflavonoids from Dalbergia odorifera T. Chen leaves[J]. Separation and Purification Technology, 2013, 115:136-144.
[16] GUO Y X, HAN J, ZHANG D Y, et al. An ammonium sulfate/ethanol aqueous two-phase system combined with ultrasonication for the separation and purification of lithospermic acid B from Salvia miltiorrhiza Bunge[J]. Ultrasonics Sonochemistry, 2012, 19(4):719-724.
[17] LIU Y, HAN J, WANG Y, et al. Selective separation of flavones and sugars from honeysuckle by alcohol/salt aqueous two-phase system and optimization of extraction process[J]. Separation and Purification Technology, 2013, 118:776-783.
[18] ZHANG D Y, ZU Y G, FU Y J, et al. Aqueous two-phase extraction and enrichment of two main flavonoids from pigeon pea roots and the antioxidant activity[J]. Separation and Purification Technology, 2013, 102:26-33.
[19] ASENJO J A, ANDREWS B A. Aqueous two-phase systems for protein separation:Phase separation and applications[J]. Journal of Chromatography A, 2012, 1238:1-10.
[20] 赵帅, 刘磊磊, 郭婕, 等.双水相气浮溶剂浮选法分离富集杜仲叶中京尼平苷酸[J]. 中草药, 2015, 46(16):2400-2406. ZHAO S, LIU L L, GUO J, et al. Separation and enrichment of geniposidic acid from leaves of Eucommia ulmoides by aqueous two-phase flotation[J]. Chinese Traditional Herb Drugs, 2015, 46(16):2400-2406.
[21] JIANG X M, LU Y M, TAN C P, et al. Combination of aqueous two-phase extraction and cation-exchange chromatography:New strategies for separation and purification of alliin from garlic powder[J]. Journal of Chromatography B, 2014, 957:60-67.
[22] FAN J P,CAO J, ZHANG X H, et al. Optimization of ionic liquid based ultrasonic assisted extraction of puerarin from radix Puerariae lobatae by response surface methodology[J]. Food Chemistry, 2012, 135(4):2299-2306.

响应面法优化超声波辅助乙醇/盐双水相萃取栀子中京尼平苷

Optimization of Ultrasonic Assisted Extraction of Geniposide from Gardenia Fruits by Using Ethanol/Salt Aqueous Two-phase System with Response Surface Method

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

投稿指南

在线期刊

相关单位

联系我们

[1]	许小向, 尹小莉, 洪艳平, 邹凯, 上官新晨, 梁强. 火炬松松针多糖提取工艺及其抗氧化性研究[J]. 林产化学与工业, 2016, 36(3): 114-120.
[2]	李鑫, 顾夕梅, 绳敖楠, 张丽, 陈晓佩, 勇强. 双水相木聚糖酶水解体系制备木二糖及其纯化[J]. 林产化学与工业, 2015, 35(5): 93-97.
[3]	夏田娟, 毕良武, 赵振东, 程贤, 邢雅丽. 迷迭香叶中鼠尾草酸的分离纯化[J]. 林产化学与工业, 2015, 35(5): 105-110.
[4]	王奎, 蒋剑春, 卢辛成, 徐俊明, 梁馨予. 超声波辅助下K₂O/C催化制备纤维低聚糖脂肪酸酯表面活性剂[J]. 林产化学与工业, 2015, 35(4): 72-78.
[5]	游凤, 黄立新, 张彩虹, 谢普军, 张耀雷. 超声波辅助减压提取枣皮多酚的研究[J]. 林产化学与工业, 2014, 34(1): 107-112.
[6]	韩东;王珏;朱兴一;谢捷. 响应面法优化水栀子多糖的闪式提取工艺[J]. 林产化学与工业, 2013, 33(6): 59-62.
[7]	彭密军;吕强;彭胜;张昌伟;张琳杰;瞿湘. 杜仲总木脂素的超声波辅助提取工艺研究[J]. 林产化学与工业, 2013, 33(4): 89-92.
[8]	李田田;王飞. 超声波辅助提取黑荆树皮原花色素工艺优化[J]. 林产化学与工业, 2012, 32(5): 56-62.
[9]	李大伟;毕良武;赵振东;李冬梅;刘先章. 迷迭香叶中鼠尾草酸超声波辅助提取的优化[J]. 林产化学与工业, 2011, 31(3): 60-64.
[10]	杜志云;涂增清;张焜;莫容清;潘文龙;汤志恺. 超声波辅助提取槐花总黄酮及其对酪氨酸酶的抑制作用[J]. 林产化学与工业, 2011, 31(3): 39-44.
[11]	李润丰;常学东;陈雪娜. 板栗多糖提取工艺的研究[J]. 林产化学与工业, 2011, 31(2): 96-100.
[12]	刘伟;黄立新;张彩虹;. 天然栀子黄色素的低温真空干燥研究[J]. 林产化学与工业, 2011, 31(1): 57-62.
[13]	陈建军;何国庆. 超声波辅助萃取法提取乌药叶黄酮类化合物的工艺研究[J]. 林产化学与工业, 2010, 30(6): 102-106.
[14]	丁为民;周丹;于涛;黄靖姝;阎秀峰;王洋. 均匀设计法优化桦木醇超声波辅助提取工艺[J]. 林产化学与工业, 2010, 30(3): 78-82.
[15]	吴冬梅;陈笳鸿;汪咏梅;徐曼;吴在嵩. 超声波辅助提取毛杨梅树皮原花色素工艺研究[J]. 林产化学与工业, 2009, 29(S1): 105-109.