党参多糖的大孔树脂纯化及其美白、吸湿保湿性能

doi:10.3969/j.issn.0253-2417.2023.03.010

林产化学与工业 ›› 2023, Vol. 43 ›› Issue (3): 79-88.doi: 10.3969/j.issn.0253-2417.2023.03.010

党参多糖的大孔树脂纯化及其美白、吸湿保湿性能

詹梦茹¹^,²^,³(), 王彦斌¹^,²^,³^,*(), 康淑荷¹^,²^,³, 罗兴平¹^,²^,³

1. 西北民族大学化工学院，甘肃兰州 730106
2. 国家民族事务委员会环境友好复合材料重点实验室，甘肃兰州 730106
3. 甘肃省生物质功能复合材料工程研究中心，甘肃兰州 730106

收稿日期:2022-03-04 出版日期:2023-06-28 发布日期:2023-06-27
通讯作者: 王彦斌 E-mail:729255126@qq.com;ybwang@126.com
作者简介:王彦斌，教授，硕士生导师，主要从事生物质材料利用与开发；E-mail：ybwang@126.com
詹梦茹(1997—)，女，河南信阳人，硕士生，主要从事天然药物的开发和利用；E-mail：729255126@qq.com
基金资助:
中央高校创新团队项目(31920190018);国家自然科学基金资助项目(21865028)

Purification of Polysaccharides from Codonopsis pilosula by Macroporous Adsorption Resin and Its Whitening, Moisture Absorption and Moisturizing Properties

Mengru ZHAN¹^,²^,³(), Yanbin WANG¹^,²^,³^,*(), Shuhe KANG¹^,²^,³, Xingping LUO¹^,²^,³

1. School of Chemical Engineering, Northwest Minzu University, Lanzhou 730106, China
2. Key Laboratory of Environment-Friendly Composites of the State Ethnic Affairs Commission, Lanzhou 730106, China
3. Gansu Provincial Biomass Function Composites Engineering Research Center, Lanzhou 730106, China

Received:2022-03-04 Online:2023-06-28 Published:2023-06-27
Contact: Yanbin WANG E-mail:729255126@qq.com;ybwang@126.com

摘要/Abstract

摘要：

基于静态实验，比较了14种不同型号大孔吸附树脂S-8、ADS-F8、LSA-21、LSA-10、HP-20、ADS-17、NKA-9、DM130、AB-8、X-5、H103、D101、XAD-8和DA201对党参多糖的纯化效果，优选出较佳树脂ADS-F8进行动态吸附。在单因素试验基础上，以多糖保留率、脱色率、蛋白质脱除率的加权综合评分作为响应值，采用响应面法优化大孔树脂纯化党参多糖的工艺条件，并对纯化后的党参多糖进行美白活性、吸湿和保湿性能测试。研究结果表明：较佳纯化工艺条件为上样质量浓度1 g/L、洗脱液体积150 mL、流速100 mL/h、上样量150 mL，此条件下纯化的综合评分为(73.12±0.06)%(n=3)，与预测值73.02%接近。纯化多糖具有良好的美白活性，对酪氨酸酶单酚酶的半数抑制质量浓度(IC₅₀)值为3.20 g/L，与美白剂苯乙基间苯二酚的抑制作用相当；对二酚酶的IC₅₀值为7.96 g/L，高于苯乙基间苯二酚的0.73 g/L；当空气湿度为43%时，纯化多糖表现出较强的吸湿性，12 h的吸湿率最高，达123.01%；纯化多糖、粗多糖及其与甘油体积比1∶1混合物在4种空气湿度下均具有较高的保湿性，特别是混合物的保湿性更佳，12 h内的保湿率达到99.95%，保湿性能明显优于纯化多糖和粗多糖。

关键词: 党参, 多糖, 大孔吸附树脂, 响应面法, 美白活性, 吸湿性, 保湿性

Abstract:

On the basis of static experiments, the purification effects of polysaccharides on 14 kinds of macroporous adsorption resins(S-8, ADS-F8, LSA-21, LSA-10, HP-20, ADS-17, NKA-9, DM130, AB-8, X-5, H103, D101, XAD-8, DA201) from Codonopsis pilosula(Franch.) Nannf. extraction were compared, and the better resin ADS-F8 was selected for dynamic adsorption. Based on the single factor experiments, the weighted comprehensive score of polysaccharides retention rate, decolorization rate and protein removal rate were used as the response values, and the response surface methodology was used to optimize the purification conditions of C. pilosula polysaccharide by macroporous resin. Meanwhile, the whitening activity, moisture absorption and moisture retention of the purified polysaccharides were tested. The results showed that the optimal purification conditions were as follows: loading concentration of 1 g/L, eluting volume of 150 mL, flow rate of 100 mL/h, and sample volume of 150 mL. Under these conditions, the comprehensive score of purification was (73.12±0.06)%(n=3), which was close to the predicted value of 73.02%. The purified polysaccharides had favorable whitening activity, and the half inhibitory mass concentration(IC₅₀) value of tyrosinase monophenase was 3.20 g/L, which was similar to the inhibitory effect of whitening agent phenylethylresorcinol. The IC₅₀ value of bisphenolase was 7.96 g/L, which is higher than phenylethylresorcinol of 0.73 g/L. When the air humidity was 43%, the purified polysaccharide showed strong hygroscopicity, and the highest moisture absorption rate was 123.01% at 12 h. In four air humidity conditions, the purified polysaccharides, crude polysaccharides and their mixture with a volume ratio 1∶1 to glycerol all had high moisture retention, especially, the moisturizing property of the mixture was better, and the moisturizing rate reached 99.95% within 12 h, which was significantly better than that of purified polysaccharide and crude polysaccharide.

Key words: Codonopsis pilosula(Franch.) Nannf., polysaccharides, macroporous adsorption resin, response surface method, whitening activity, hygroscopicity, moisture retention

中图分类号:

TQ35

詹梦茹, 王彦斌, 康淑荷, 罗兴平. 党参多糖的大孔树脂纯化及其美白、吸湿保湿性能[J]. 林产化学与工业, 2023, 43(3): 79-88.

Mengru ZHAN, Yanbin WANG, Shuhe KANG, Xingping LUO. Purification of Polysaccharides from Codonopsis pilosula by Macroporous Adsorption Resin and Its Whitening, Moisture Absorption and Moisturizing Properties[J]. Chemistry and Industry of Forest Products, 2023, 43(3): 79-88.

图/表 8

表1

表2

图1

表3

表4

图2

图3

图4

参考文献 25

1	中国药典委员会. 中华人民共和国中国药典(2020年版)[M]. 北京: 中国医药科技出版社, 2020.
	Chinese Pharmacopoeia Commission . Pharmacopoeia of the People's Republic of China 2020[M]. Beijing: China Medical Science Press, 2020.
2	李成义, 刘书斌, 李硕, 等. 甘肃党参栽培现状调查分析[J]. 中国现代中药, 2016, 18 (1): 102- 105.
	LI C Y , LIU S B , LI S , et al. Investigation and analysis of status cultivation of Gansu Codonopsis radix[J]. Modern Chinese Medicine, 2016, 18 (1): 102- 105.
3	窦武宇, 崔治家, 侯嘉, 等. 甘肃不同产地党参药材中多糖含量测定[J]. 中兽医医药杂志, 2016, 35 (3): 55- 59.
	DOU W Y , CUI Z J , HOU J , et al. The content of polysaccharide in the medicinal herbs Codonopsis pilosula of different origin of gansu province[J]. Journal of Traditional Chinese Veterinary Medicine, 2016, 35 (3): 55- 59.
4	LUAN F, JI Y F, PENG L X, et al. Extraction, purification, structural characteristics and biological properties of the polysaccharides from Codonopsis pilosula: A review[J/OL]. Carbohydrate Polymers, 2021, 261: 117863[2022-01-25]. https://doi.org/10.1016/j.carbpol.2021.117863.
5	NAMJOYAN F , FARASAT M , ALISHAHI M , et al. The anti-melanogenesis activities of some selected red macroalgae from northern coasts of the persian gulf[J]. Iranian Journal of Pharmaceutical Research, 2018, 18 (1): 383- 390.
6	于承仟, 徐学刚, 李远宏. 苯乙基间苯二酚的研究应用进展[J]. 中国皮肤性病学杂志, 2020, 34 (6): 692- 695.
	YU C Q , XU X G , LI Y H . Research progress of phenylethyl resorcinol[J]. The Chinese Journal of Dermatovenereology, 2020, 34 (6): 692- 695.
7	许雷, 李湛, 周火兰, 等. 酶解褐藻胶寡糖的吸湿及保湿性能研究[J]. 日用化学工业, 2011, 41 (1): 42- 45.
	XU L , LI Z , ZHOU H L , et al. Study of hygroscopic and moisturizing performance of oligosaccharides obtained from enzymeolysis of algin[J]. China Surfactant Detergent & Cosmetics, 2011, 41 (1): 42- 45.
8	BERTHON J Y , NACHAT-KAPPES R , BEY M , et al. Marine algae as attractive source to skin care[J]. Free Radical Research, 2017, 51 (6): 555- 567. doi: 10.1080/10715762.2017.1355550
9	刘冰月, 刘学, 邬凤娟, 等. 羊栖菜岩藻多糖的提取工艺优化及保湿性能[J]. 日用化学工业, 2017, 47 (7): 398- 402.
	LIU B Y , LIU X , WU F J , et al. Optimization of process technological conditions for obtaining fucoidan from Sargassum fusiforme and detection of its moisturizing performance[J]. China Surfactant Detergent & Cosmetics, 2017, 47 (7): 398- 402.
10	PRIYAN SHANURA FERNANDO I , KIM K N , KIM D , et al. Algal polysaccharides: Potential bioactive substances for cosmeceutical applications[J]. Critical Reviews in Biotechnology, 2019, 39 (1): 99- 113.
11	ZOU Y , CHEN X , YANG W , et al. Response surface methodology for optimization of the ultrasonic extraction of polysaccharides from Codonopsis pilosula Nannf. var. modesta L.T. Shen[J]. Carbohydrate Polymers, 2011, 84 (1): 503- 508.
12	彭焱, 唐克华, 董爱文. 大孔树脂纯化白及多酚及其体外抗氧化活性研究[J]. 林产化学与工业, 2019, 39 (4): 91- 99.
	PENG Y , TANG K H , DONG A W . Purification of Bletilla striata by macroporous resin and its antioxidant activity in vitro[J]. Chemistry and Industry of Forest Products, 2019, 39 (4): 91- 99.
13	HU Z Y , ZHOU H L , LI Y P , et al. Optimized purification process of polysaccharides from Carex meyeriana Kunth by macroporous resin, its characterization and immunomodulatory activity[J]. International Journal of Biological Macromolecules, 2019, 132, 76- 86.
14	刘冲英, 周宁, 崔涛, 等. 响应面法优化大孔树脂纯化地黄多糖工艺[J]. 食品工业科技, 2021, 42 (6): 202- 207.
	LIU C Y , ZHOU N , CUI T , et al. Optimization of purification process of polysaccharides from Rehmannia glutinosa Libosch. with macroporous adsorption resins by response surface methodology[J]. Science and Technology of Food Industry, 2021, 42 (6): 202- 207.
15	刘富岗, 李汉伟, 郑群杰, 等. 响应面法优选泽兰多糖的大孔吸附树脂纯化工艺[J]. 暨南大学学报(自然科学与医学版), 2019, 40 (1): 1- 9.
	LIU F G , LI H W , ZHENG Q J , et al. Optimization of purification process of Lycopus lucidus polysaccharide with macroporous adsorption resins by response surface method[J]. Journal of Jinan University(Natural Science & Medicine Edition), 2019, 40 (1): 1- 9.
16	陈丽娟. 银耳多糖快速提取及可控降解的研究[D]. 广州: 华南理工大学, 2017.
	CHEN L J. Research on fast extraction and controlled degradation of polysaccharides from tremella[D]. Guangzhou: South China University of Technology, 2017.
17	WU Q Y , QU H S , JIA J Q , et al. Characterization, antioxidant and antitumor activities of polysaccharides from purple sweet potato[J]. Carbohydrate Polymers, 2015, 132, 31- 40.
18	范敬宜. 牡丹黄酮的膜法提纯及其美白活性研究[D]. 北京: 北京化工大学, 2020.
	FAN J Y. Purification of flavonoids from peony by membrane method and its whitening activity[D]. Beijing: Beijing University of Chemical Technology, 2020.
19	郭子叶. 浒苔多糖化妆品开发潜力研究[D]. 上海: 上海海洋大学, 2014.
	GUO Z Y. Potential use of polysaccharides from Ulva prolifera in cosmetic[D]. Shanghai: Shanghai Ocean University, 2014.
20	刘大伟. 黄芩多糖大孔树脂纯化工艺优化及其抑菌活性[J]. 食品工业科技, 2021, 42 (15): 183- 188.
	LIU D W . Optimization purification process of polysaccharides from Scutellaria baicalensis Georgi by macroporous resins and its antibacterial activity[J]. Science and Technology of Food Industry, 2021, 42 (15): 183- 188.
21	赵丹洁, 唐鹏, 田冬梅, 等. 金线莲多糖纯化工艺及其抗氧化活性研究[J]. 食品研究与开发, 2021, 42 (20): 116- 123.
	ZHAO D J , TANG P , TIAN D M , et al. Study on purification technology and antioxidant activity of polysaccharides from Anoectochilus roxburghii[J]. Food Research and Development, 2021, 42 (20): 116- 123.
22	GUO L, LI X M, ZHANG J, et al.Optimization of extraction and macroporous resin purification processes of total triterpenoid from Boletus edulis Bull.: Fr.[J/OL]. Journal of Food Processing and Preservation, 2021, 45(4): e15292[2022-01-25]. https://doi.org/10.1111/jfpp.15292.
23	孙伟, 叶润, 蔡静, 等. 大孔树脂纯化桑白皮多糖的工艺研究[J]. 食品工业科技, 2020, 41 (14): 129- 133.
	SUN W , YE R , CAI J , et al. Purification of polysaccharide from mori cortex by macroporous resin[J]. Science and Technology of Food Industry, 2020, 41 (14): 129- 133.
24	谷仿丽, 姜雪萍, 陈艳君, 等. 霍山石斛多糖吸湿保湿性能及皮肤刺激性研究[J]. 天然产物研究与开发, 2018, 30 (10): 1701- 1705.
	GU F L , JIANG X P , CHEN Y J , et al. Study on moisture-absorption-retention capacity and skin irritation of polysaccharide from Dendrobium huoshanense[J]. Natural Product Research and Development, 2018, 30 (10): 1701- 1705.
25	方园园, 邓校娟, 张俊, 等. 银耳菌丝体多糖理化性质及其吸湿保湿性能测定[J]. 安徽科技学院学报, 2020, 34 (1): 37- 42.
	FANG Y Y , DENG X J , ZHANG J , et al. Identification of physicochemical properties, hygroscopic and moisturizing properties of polysaccharide from mycelium of Tremella fuciformis[J]. Journal of Anhui Science and Technology University, 2020, 34 (1): 37- 42.

成分ingredient	用量dosage/mL
成分ingredient	Z₁组group Z₁	Z₂组group Z₂	Z₃组group Z₃	Z₄组group Z₄
样品溶液sample solution	1	1	0	0
PBS	2	3	3	4
酪氨酸酶tyrosinase	1	0	1	0
L-酪氨酸/L-多巴L-tyrosine/L-dopa	1	1	1	1

树脂型号resin type	吸附率/% adsorption rate	解吸率/% desorption rate
H103	65.39±0.36	47.61±0.80
DM130	67.56±0.32	36.09±2.07
ADS-F8	72.99±0.09	54.81±1.28
HP-20	67.46±0.22	25.32±0.93
S-8	50.45±0.54	29.54±0.87
XAD-8	49.77±0.56	12.50±1.47
LSA-21	68.41±0.79	32.27±2.69
ADS-17	68.74±0.44	29.04±2.52
D101	60.67±1.39	37.40±0.86
X-5	62.58±0.87	37.90±1.56
NKA-9	67.99±0.75	22.75±1.54
AB-8	55.26±0.26	40.28±1.12
LSA-10	69.38±1.16	28.45±1.11
DA201	60.14±1.20	9.86±1.48

序号No.	X₁上样质量浓度/(g?L^-1)sample mass concentration	X₂洗脱液体积/mL effluent volume	X₃流速/(mL?h^-1)flow rate	综合评分(Y)/% comprehensive evaluation
1	1.5	200	100	44.91
2	1.0	150	100	75.31
3	1.5	100	100	54.02
4	1.0	100	150	48.21
5	0.5	100	100	52.92
6	1.0	150	100	72.02
7	1.5	150	50	56.86
8	1.0	150	100	69.65
9	1.5	150	150	48.92
10	0.5	150	50	54.3
11	0.5	200	100	49.61
12	1.0	200	150	47.00
13	1.0	150	100	74.26
14	1.0	100	50	51.00
15	1.0	200	50	52.65
16	0.5	150	150	51.38
17	1.0	150	100	72.93

方差来源soruces of variation	平方和quadratic sum	自由度degree of freedom	均方mean square	F值F-value	P值P-value	显著性significant
模型model	1 790.29	9	198.92	33.08	< 0.000 1	**
X₁	1.53	1	1.53	0.25	0.629 3
X₂	17.94	1	17.94	2.98	0.127 7
X₃	46.56	1	46.56	7.74	0.027 2	*
X₁X₂	8.41	1	8.41	1.40	0.275 6
X₁X₃	6.30	1	6.30	1.05	0.340 1
X₂X₃	2.04	1	2.04	0.34	0.578 1
X₁²	392.87	1	392.87	65.34	< 0.000 1	**
X₂²	690.88	1	690.88	114.90	< 0.000 1	**
X₃²	447.52	1	447.52	74.43	< 0.000 1	**
残差residual	42.09	7	6.01
失拟误差lack of fit	23.12	3	7.71	1.62	0.317 8
纯误差pure error	18.97	4	4.74
总和total	1 832.38	16

[1]	张梅, 张燕杰, 陈为健, 井华芳, 娄本勇. 锦绣杜鹃花色苷微胶囊的制备及其稳定性研究[J]. 林产化学与工业, 2023, 43(2): 19-26.
[2]	颜洋洋, 张昌伟, 李川, 周昊, 王成章. 不同纯度靛蓝提取物的制备及其抑菌活性研究[J]. 林产化学与工业, 2023, 43(1): 104-112.
[3]	付佳, 吴禹践, 刘志明, 王海英. 桑叶乙醇提取物化学成分分析及提取工艺优化[J]. 林产化学与工业, 2023, 43(1): 120-126.
[4]	陈默然, 于华忠, 刘磊磊, 唐克华. 漂洗降低白及militarine含量的响应面法研究[J]. 林产化学与工业, 2022, 42(6): 117-122.
[5]	李胜男, 程贤, 毕良武, 曾维星, 陈玉湘, 赵振东. 肉桂多糖的结构分析及抗氧化活性研究[J]. 林产化学与工业, 2022, 42(3): 34-40.
[6]	黄天, 张晓彤, 赵江琳, 郭健铭, 周鑫, 徐勇. 木糖酸辅助水解木聚糖制备低聚木糖及其分离与回收工艺研究[J]. 林产化学与工业, 2021, 41(5): 8-14.
[7]	黄维, 郭梦云, 王玉启, 林金垒, 张宇翔, 邹双全. 圆齿野鸦椿果皮多糖的提取及其抗炎活性研究[J]. 林产化学与工业, 2021, 41(3): 77-84.
[8]	陈淑琴, 叶燕燕, 全颖萱, 李林海, 倪林, 邹双全. 圆齿野鸦椿籽油提取工艺及脂肪酸组分分析[J]. 林产化学与工业, 2021, 41(1): 53-59.
[9]	蒋建新, 朱莉伟, 张伟, 李先平. 预处理温度对圆皂荚多糖胶制备与性能的影响[J]. 林产化学与工业, 2020, 40(6): 1-7.
[10]	张爱华,唐娟,赖鹏英,何怡丹,黎继烈,肖志红. 光皮梾木油热解产物催化加氢制备生物燃料[J]. 林产化学与工业, 2020, 40(5): 43-49.
[11]	张婷婷,韩秀丽,刘莹,董春玲,常春. 生物质基活性炭对环丙沙星的吸附性能研究[J]. 林产化学与工业, 2020, 40(4): 71-78.
[12]	李子江,刘慰,吴磊,王丹,司传领. 偃松松塔多糖的单糖组成分析及活性研究[J]. 林产化学与工业, 2020, 40(1): 37-44.
[13]	许嘉琍,张海波,姚姝凤,高宏,商士斌. 林业生物质基果蔬涂膜保鲜剂的研究进展[J]. 林产化学与工业, 2019, 39(3): 10-16.
[14]	张笮晦,钱信怡,黄广智,毕良武. 肉桂和同源根皮不同极性部位的成分分析及其抑菌效果[J]. 林产化学与工业, 2019, 39(3): 115-122.
[15]	冯书晓,张洪涛,王水永,胡永鑫,朱培文,尹卫平. 无花果果实水溶性成分的分离与鉴定[J]. 林产化学与工业, 2019, 39(2): 33-38.

党参多糖的大孔树脂纯化及其美白、吸湿保湿性能

Purification of Polysaccharides from Codonopsis pilosula by Macroporous Adsorption Resin and Its Whitening, Moisture Absorption and Moisturizing Properties

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 25

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

投稿指南

在线期刊

相关单位

联系我们