大孔吸附树脂提甜茶苷的研究

林产化学与工业 ›› 2008, Vol. 28 ›› Issue (5): 35-39.

大孔吸附树脂提甜茶苷的研究

周如金, 黄敏, 张玲, 马玉刚, 李春海

茂名学院化学与生命科学学院, 广东, 茂名, 525000

收稿日期:2007-10-15 修回日期:1900-01-01 出版日期:2008-10-30 发布日期:2008-10-30

Study on Extraction of Rubusoside from Rubus suavissimus S. Lee by Macroporous Resins Adsorption

ZHOU Ru-jin, HUANG Min, ZHANG Ling, MA Yu-gang, LI Chun-hai

College of Chemistry & Life Science, Maoming University, Maoming 525000, China

Received:2007-10-15 Revised:1900-01-01 Online:2008-10-30 Published:2008-10-30

摘要/Abstract

摘要： 从6种大孔树脂中筛选了适宜的提取分离甜茶苷的吸附剂,研究了大孔吸附树脂提取甜茶苷的工艺,包括甜茶苷溶液的质量浓度、pH值和流速对吸附过程的影响,解吸剂及解吸温度对解吸过程的影响.结果表明:AB-8树脂是理想的甜茶苷吸附剂,其吸附甜茶苷较理想的工艺条件是:原料液质量浓度约为7.7mg/L,pH值约为8,流速为3BV/h(BV为层析柱中树脂床的体积);理想的洗脱条件为:流速为3BV/h,室温下以70%乙醇溶液为洗脱剂,用量为BV,或40℃下,以60%乙醇溶液为洗脱剂,用量为4BV.实验室利用该工艺成功地分离出甜茶苷.

关键词: 大孔吸附树脂, 提取, 甜茶苷

Abstract: Adsorption of rubusoside from Rubus suavissimus S. Lee by 6 kinds of macroporous resins were tested to obtain the best adsorbent. Some of factors, not only on adsorption of rubusoside including the concentration of rubusoside, pH value and flow rate of solution, but also on desorption such as selection of desorbents and temperature, were investigated. The results showed that AB-8 resin was the best adsorbent for adsorption of rubusoside from R.suavissimus among 6 kinds of macroporous resins. The optimum conditions for adsorption by AB-8 resin were as follows: rubusoside solution concentration 7.7mg/L, pH value 8 and flow rate 3 BV/h. For desorption, ethanol solution showed good performance and temperature had obvious influence so both processes concerned were gained to complete desorption of rubusoside from adsorbent. One process can be operated under the conditions of 70% ethanol solution as desorbent, flow rate 3 BV/h(BV means the resin bed volume on chromatography column), total desorbent 5 BV at room temperature, or under the conditions of 60% ethanol solution as desorbent, flow rate 3 BV/h, total desorbent 4 BV at 40℃. Rubusoside was obtained successfully from R. suavissimus by this process in our laboratory.

Key words: macroporous adsorption resin, extraction, rubusoside

中图分类号:

周如金;黄敏;张玲;马玉刚;李春海. 大孔吸附树脂提甜茶苷的研究[J]. 林产化学与工业, 2008, 28(5): 35-39.

ZHOU Ru-jin;HUANG Min;ZHANG Ling;MA Yu-gang;LI Chun-hai. Study on Extraction of Rubusoside from Rubus suavissimus S. Lee by Macroporous Resins Adsorption[J]. , 2008, 28(5): 35-39.

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